Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GSS20_RS14915 Genome accession   NZ_CP047995
Coordinates   3193334..3194563 (-) Length   409 a.a.
NCBI ID   WP_058664673.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20150710009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3188334..3199563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS20_RS14885 (GSS20_15655) - 3188519..3188929 (+) 411 WP_005460376.1 flagellar basal body-associated protein FliL -
  GSS20_RS14890 (GSS20_15660) glpG 3188989..3189831 (-) 843 WP_140391908.1 rhomboid family intramembrane serine protease GlpG -
  GSS20_RS14895 (GSS20_15665) glpE 3189831..3190151 (-) 321 WP_005460380.1 thiosulfate sulfurtransferase GlpE -
  GSS20_RS14900 (GSS20_15670) rpoH 3190643..3191503 (-) 861 WP_005490420.1 RNA polymerase sigma factor RpoH -
  GSS20_RS14905 (GSS20_15675) ftsX 3191679..3192647 (-) 969 WP_005481956.1 permease-like cell division protein FtsX -
  GSS20_RS14910 (GSS20_15680) ftsE 3192637..3193311 (-) 675 WP_005481953.1 cell division ATP-binding protein FtsE -
  GSS20_RS14915 (GSS20_15685) pilA 3193334..3194563 (-) 1230 WP_058664673.1 signal recognition particle-docking protein FtsY Machinery gene
  GSS20_RS14920 (GSS20_15690) rsmD 3194787..3195386 (+) 600 WP_140391911.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GSS20_RS14925 (GSS20_15695) - 3195412..3195678 (+) 267 WP_005496405.1 DUF1145 domain-containing protein -
  GSS20_RS14930 (GSS20_15700) - 3195732..3196316 (-) 585 WP_005455144.1 YhgN family NAAT transporter -
  GSS20_RS14935 (GSS20_15705) - 3196436..3196657 (-) 222 WP_162457633.1 YecH family metal-binding protein -
  GSS20_RS14940 (GSS20_15710) - 3196727..3197347 (-) 621 WP_005481960.1 lysoplasmalogenase -
  GSS20_RS14945 (GSS20_15725) - 3198326..3198694 (-) 369 WP_005489749.1 hypothetical protein -
  GSS20_RS14950 (GSS20_15730) - 3198936..3199265 (+) 330 WP_015297474.1 DUF2500 domain-containing protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44541.11 Da        Isoelectric Point: 4.2559

>NTDB_id=366966 GSS20_RS14915 WP_058664673.1 3193334..3194563(-) (pilA) [Vibrio parahaemolyticus strain 20150710009]
MTEKKKRGLLSWLGFGDEEQSPKPKTEETVTEEAVEAPSEEQQTEEVAEQAQQTQELEQEPEKAEAAPAEAEAEAEAEAE
AEAEAEEPQVPVAPRIQEQEKPTESFFARLKRSLSRTKANIGAGFFGLFSGKKIDDDLFEELEEQLLIADVGMDTTTKII
NNLTEKASRGDLKDGEALYGLLKEEMAEILSKVEQPLDIDSSKTPYVILMVGVNGVGKTTTIGKLAKQFQSQGKKVMLAA
GDTFRAAAVEQLQVWGERNNVPVIAQHTGADSASVIYDAIEAAKARGVDVVIADTAGRLQNKANLMEELRKIVRVMKKID
DSAPHEIMLTLDAGTGQNAISQAKLFSDVAPLTGITLTKLDGTAKGGVIFAIADQFSIPIRYIGVGEGIEDLRPFETQEF
IDALFSREE

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=366966 GSS20_RS14915 WP_058664673.1 3193334..3194563(-) (pilA) [Vibrio parahaemolyticus strain 20150710009]
ATGACGGAAAAAAAGAAGCGCGGATTACTTTCGTGGCTAGGTTTTGGTGACGAAGAACAAAGCCCAAAACCAAAAACTGA
AGAAACAGTAACAGAAGAAGCAGTGGAAGCGCCTTCTGAAGAGCAGCAAACCGAAGAGGTTGCTGAGCAAGCTCAACAAA
CTCAAGAGCTTGAACAAGAGCCAGAAAAAGCTGAGGCTGCCCCAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAA
GCAGAAGCAGAAGCAGAAGAGCCACAAGTACCGGTTGCGCCTCGTATTCAAGAGCAAGAAAAGCCGACAGAAAGCTTCTT
CGCTCGCCTTAAGCGCAGCCTAAGCCGTACGAAAGCAAACATCGGTGCTGGTTTCTTTGGTCTGTTCAGCGGTAAAAAAA
TCGATGACGACCTATTTGAAGAGCTAGAAGAGCAACTGCTCATTGCCGATGTGGGCATGGACACCACCACAAAAATCATC
AACAACCTGACAGAAAAAGCCTCTCGTGGTGATCTGAAAGATGGCGAAGCCCTTTATGGTCTGCTGAAAGAAGAAATGGC
GGAGATTCTATCTAAAGTAGAACAGCCTCTAGATATCGACAGTAGCAAAACACCTTATGTCATCTTGATGGTTGGTGTGA
ATGGTGTGGGTAAAACCACCACCATCGGTAAACTTGCGAAGCAGTTCCAAAGCCAAGGCAAGAAAGTGATGTTAGCGGCT
GGCGATACTTTCCGAGCGGCTGCAGTTGAGCAGCTGCAAGTGTGGGGCGAGCGTAACAATGTTCCTGTGATCGCGCAGCA
TACTGGCGCAGATAGCGCATCGGTTATCTACGATGCGATTGAAGCGGCAAAAGCGCGTGGCGTAGATGTGGTTATCGCCG
ATACGGCGGGTCGTCTACAAAACAAGGCCAACCTAATGGAAGAGCTACGCAAGATTGTGCGTGTGATGAAGAAAATTGAT
GATTCTGCACCACATGAAATCATGCTGACGCTGGATGCAGGTACTGGCCAAAATGCGATTAGCCAAGCGAAACTTTTCAG
TGATGTAGCTCCTCTAACCGGGATTACATTGACTAAGCTGGATGGTACAGCGAAAGGCGGCGTTATTTTCGCTATCGCTG
ATCAGTTTAGTATTCCAATTCGCTACATTGGTGTTGGCGAAGGCATTGAAGACTTGCGTCCTTTTGAAACTCAAGAGTTT
ATCGACGCTTTGTTTAGCCGTGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(125-186)

SRP54-type protein, GTPase domain (SRP54)

(206-405)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.072

92.176

0.452