Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSS20_RS13515 Genome accession   NZ_CP047995
Coordinates   2893515..2895239 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain 20150710009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2888515..2900239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS20_RS13480 (GSS20_14160) - 2888804..2889517 (-) 714 WP_005444351.1 PilW family protein -
  GSS20_RS13485 (GSS20_14165) - 2889517..2890098 (-) 582 WP_140391543.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GSS20_RS13490 (GSS20_14170) - 2890095..2890643 (-) 549 WP_062889603.1 type II secretion system protein -
  GSS20_RS13495 (GSS20_14175) - 2890763..2890993 (-) 231 WP_005432800.1 type II secretion system protein -
  GSS20_RS13500 (GSS20_14180) - 2891071..2891523 (-) 453 WP_025625399.1 type II secretion system protein -
  GSS20_RS13505 (GSS20_14185) - 2891818..2892279 (-) 462 WP_053321356.1 hypothetical protein -
  GSS20_RS13510 (GSS20_14190) - 2892283..2893506 (-) 1224 WP_021484749.1 type II secretion system F family protein -
  GSS20_RS13515 (GSS20_14195) pilB 2893515..2895239 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  GSS20_RS13520 (GSS20_14200) - 2895236..2896312 (-) 1077 WP_161613039.1 tetratricopeptide repeat protein -
  GSS20_RS13525 (GSS20_14205) - 2896309..2897154 (-) 846 WP_140391544.1 ExeA family protein -
  GSS20_RS13530 (GSS20_14210) mshL 2897198..2898841 (-) 1644 WP_005456314.1 pilus (MSHA type) biogenesis protein MshL -
  GSS20_RS13535 (GSS20_14215) - 2898862..2899191 (-) 330 WP_140391545.1 MSHA biogenesis protein MshK -
  GSS20_RS13540 (GSS20_14220) gspM 2899184..2899834 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=366954 GSS20_RS13515 WP_005456306.1 2893515..2895239(-) (pilB) [Vibrio parahaemolyticus strain 20150710009]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=366954 GSS20_RS13515 WP_005456306.1 2893515..2895239(-) (pilB) [Vibrio parahaemolyticus strain 20150710009]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGCATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGACCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTTCATGCTCGTCGTTTACGTGCCATGGTGGTGGCTCGCAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATTGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGACGTACCAAAGAGATCGCGTCCTTTGCTGAGCAGTTACAAGCTGAG
CACCAAGACGTACAAAGCTTTGACTATGGTATCGATGAAGCAGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTCCTGCGTTTACGCCAGC
GTGTCGATGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCGACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGCTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGTCGTCAGTTATCTCGTCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAAATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGTATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CACGCGTGCTACGCACCTTCTTACGCCAAGACCCAGACATCATTTTGATTGGTGAGATGCGTGATCAAGAGACGGTAGAG
ATTGGCTTACGAGCAGCATTAACTGGTCACTTAGTATTGAGTACGTTGCATACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACTCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAATTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGTGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCGCTGCAAGGTGCTGTGAGCCTGGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361