Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FGM04_RS15660 Genome accession   NZ_CP040717
Coordinates   3377069..3378508 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain HX3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3372069..3383508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGM04_RS15630 (FGM04_15650) mlaF 3372646..3373446 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  FGM04_RS15635 (FGM04_15655) - 3373654..3374649 (+) 996 WP_157161835.1 KpsF/GutQ family sugar-phosphate isomerase -
  FGM04_RS15640 (FGM04_15660) kdsC 3374649..3375203 (+) 555 WP_157161836.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FGM04_RS15645 (FGM04_15665) lptC 3375200..3375760 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  FGM04_RS15650 (FGM04_15670) lptA 3375747..3376274 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  FGM04_RS15655 (FGM04_15675) lptB 3376278..3377003 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  FGM04_RS15660 (FGM04_15680) rpoN/rpoN1 3377069..3378508 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  FGM04_RS15665 (FGM04_15685) hpf 3378529..3378816 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  FGM04_RS15670 (FGM04_15690) ptsN 3378819..3379265 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FGM04_RS15675 (FGM04_15695) rapZ 3379311..3380177 (+) 867 WP_021231839.1 RNase adapter RapZ -
  FGM04_RS15680 (FGM04_15700) npr 3380198..3380470 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  FGM04_RS15685 (FGM04_15705) - 3380488..3382392 (-) 1905 WP_157161837.1 transcription antiterminator -
  FGM04_RS15690 (FGM04_15710) dagF 3382500..3383240 (-) 741 WP_157161838.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=366773 FGM04_RS15660 WP_005336331.1 3377069..3378508(+) (rpoN/rpoN1) [Aeromonas veronii strain HX3]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=366773 FGM04_RS15660 WP_005336331.1 3377069..3378508(+) (rpoN/rpoN1) [Aeromonas veronii strain HX3]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GATGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCTGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCAGCCGTCACCAGCGATGACGTAGAGGTGGAGCTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGCCCCGGCAATGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment