Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GSS61_RS12505 Genome accession   NZ_CP047985
Coordinates   2639666..2641390 (-) Length   574 a.a.
NCBI ID   WP_005456306.1    Uniprot ID   Q87LB1
Organism   Vibrio parahaemolyticus strain HZ-52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2634666..2646390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSS61_RS12470 (GSS61_13035) - 2634974..2635735 (-) 762 WP_029845938.1 PilW family protein -
  GSS61_RS12475 (GSS61_13040) - 2635735..2636298 (-) 564 WP_062851426.1 type IV pilus modification PilV family protein -
  GSS61_RS12480 (GSS61_13045) - 2636295..2636756 (-) 462 WP_025545966.1 type II secretion system protein -
  GSS61_RS12485 (GSS61_13050) - 2636866..2637090 (-) 225 WP_012841374.1 type II secretion system protein -
  GSS61_RS12490 (GSS61_13055) - 2637168..2637674 (-) 507 WP_029845939.1 type II secretion system protein -
  GSS61_RS12495 (GSS61_13060) - 2637969..2638430 (-) 462 WP_005456302.1 MSHA biogenesis protein MshF -
  GSS61_RS12500 (GSS61_13065) - 2638434..2639657 (-) 1224 WP_015297334.1 type II secretion system F family protein -
  GSS61_RS12505 (GSS61_13070) pilB 2639666..2641390 (-) 1725 WP_005456306.1 GspE/PulE family protein Machinery gene
  GSS61_RS12510 (GSS61_13075) - 2641387..2642463 (-) 1077 WP_025611170.1 tetratricopeptide repeat protein -
  GSS61_RS12515 (GSS61_13080) - 2642460..2643305 (-) 846 WP_005497278.1 ExeA family protein -
  GSS61_RS12520 (GSS61_13085) mshL 2643349..2644992 (-) 1644 WP_024701141.1 pilus (MSHA type) biogenesis protein MshL -
  GSS61_RS12525 (GSS61_13090) - 2645013..2645342 (-) 330 WP_005481006.1 MSHA biogenesis protein MshK -
  GSS61_RS12530 (GSS61_13095) gspM 2645335..2645985 (-) 651 WP_005497276.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63886.21 Da        Isoelectric Point: 5.2324

>NTDB_id=366770 GSS61_RS12505 WP_005456306.1 2639666..2641390(-) (pilB) [Vibrio parahaemolyticus strain HZ-52]
MKIQLRKRLGDLLVEEGIVSEDQIQQALSAQRSTGQKLGDALIDLGFITEKQMLEFLSQQLGLPLIDLGRAPVDADAVQI
LPEVHARRLRAMVVARNGDTLRVAMSDPADLFTQESLMNLLGEYNLEFIIASERQLISSFDRYYRRTKEIASFAEQLQAE
HQDVQSFDYGIDEADSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETILNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRPLEESGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTQDHLDESRQQWLAGRFPNQV
GVTFHKGAGCQNCNLTGYRGRIGVFEMLELEHEMMDKLRANDAVGFAQAARRSENYKPLLASAMELALQGAVSLDEVMTL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=366770 GSS61_RS12505 WP_005456306.1 2639666..2641390(-) (pilB) [Vibrio parahaemolyticus strain HZ-52]
ATGAAAATTCAGTTGAGAAAACGCTTAGGTGACCTACTTGTTGAAGAAGGTATCGTATCGGAAGATCAAATCCAGCAAGC
TCTATCCGCTCAGCGCAGTACCGGACAAAAATTAGGTGATGCCCTGATTGATCTCGGGTTTATTACCGAAAAGCAAATGC
TGGAGTTCTTGTCTCAGCAACTTGGATTACCGTTGATCGATCTTGGACGCGCTCCAGTGGATGCGGATGCGGTGCAAATA
CTGCCAGAAGTGCATGCTCGTCGTTTACGTGCAATGGTGGTGGCTCGTAATGGTGACACTTTACGTGTTGCGATGAGTGA
TCCGGCGGATCTGTTTACCCAAGAGTCTTTAATGAACTTGCTAGGTGAGTACAACCTTGAGTTCATCATAGCCTCTGAGC
GTCAGTTGATCAGCAGTTTCGACCGTTACTACCGCCGTACCAAAGAGATCGCGTCGTTTGCTGAGCAGTTACAAGCCGAG
CACCAAGACGTACAAAGCTTTGACTATGGTATCGATGAAGCAGACAGTGAAGAAGTCACCGTTGTAAAACTGGTTAATTC
GATGTTTGAAGACGCGGTGCAAGTTGGTGCATCGGATATCCATATCGAACCAGACGATAAAGTTTTGCGTTTACGCCAGC
GTGTCGATGGTGTGCTGCATGAAACCATTCTGAACGAAGTGAACATCGCTTCTGCATTGGTATTACGCCTTAAGTTGATG
GCGCACTTGGATATTTCTGAGAAGCGTCTGCCACAAGATGGTCGCTTCAACATCAAAGTTCGTGGTCAATCGATTGATAT
TCGTATGTCGACGTTACCAACTCAGTACGGTGAATCGGTCGTAATGCGTCTGCTTAACCAGTCGTCAGGTTTACGTCCAT
TAGAAGAATCGGGTTTACCGCCTGAGCTACTGGCGCGCTTGCGCCGTCAGTTATCTCGCCCTCATGGCATGATTTTGGTA
ACTGGCCCTACGGGATCGGGTAAAACCACGACACTTTACGGCGCGTTGAGTGAGCTCAATGAACCGGGTAAAAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGTTTACCACGCATCACGCAAGTACAGATCAACAGCAAGATCGACTTAACGTTTT
CGCGCGTTCTACGCACCTTCTTGCGTCAAGACCCTGACATCATTTTGATTGGTGAGATGCGTGACCAAGAGACGGTAGAG
ATTGGCTTGCGAGCAGCACTAACCGGCCACTTAGTATTGAGTACGTTGCACACCAACGATGCGGTCGACAGCGCACTGCG
TATGATCGACATGGGGGCGCCAGGCTATCTCGTTGCCAGTGCCGTGCGTGCCGTTGTAGCTCAGCGATTGGTTCGCCGTG
TTTGTCCAGATTGTAAAACCCAAGATCATCTCGATGAGTCGCGTCAACAATGGTTAGCGGGGCGTTTTCCAAATCAAGTT
GGGGTGACTTTCCACAAAGGAGCAGGTTGTCAAAACTGTAACTTGACGGGGTATCGAGGTCGAATCGGTGTGTTTGAAAT
GCTTGAGCTGGAACATGAGATGATGGACAAGCTGCGCGCGAATGATGCGGTTGGCTTTGCACAAGCGGCTCGACGTTCAG
AAAACTACAAACCGCTTCTTGCCTCAGCAATGGAGCTTGCACTGCAAGGTGCTGTGAGCCTAGATGAAGTGATGACCCTC
GGCGAGGGTGACGCTTCGGGTAAAACTGATCCCATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.819

97.387

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.272

100

0.361