Detailed information NTG0036677

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: MRUB_RS04810
• Genome accession: NC_013946
• Coordinates: 935623..938280 (+)
• Length: 885 a.a.
• NCBI ID: WP_013013233.1
• Uniprot ID: D3PQI1
• Organism: Meiothermus ruber DSM 1279
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 930623..943280

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MRUB_RS04790 (Mrub_0945)	gap	931659..932651 (-)	993	WP_013013229.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
MRUB_RS04795 (Mrub_0946)	-	932854..934017 (-)	1164	WP_013013230.1	A24 family peptidase	-
MRUB_RS04800 (Mrub_0947)	pgeF	934194..934928 (+)	735	WP_013013231.1	peptidoglycan editing factor PgeF	-
MRUB_RS04805 (Mrub_0948)	-	934966..935523 (+)	558	WP_013013232.1	YqeG family HAD IIIA-type phosphatase	-
MRUB_RS04810 (Mrub_0949)	pilF	935623..938280 (+)	2658	WP_013013233.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
MRUB_RS04815 (Mrub_0950)	-	938378..939736 (+)	1359	WP_013013234.1	dipeptidase	-
MRUB_RS04820 (Mrub_0951)	-	939881..941110 (+)	1230	WP_013013235.1	PEGA domain-containing protein	-
MRUB_RS04825 (Mrub_0952)	-	941275..942183 (-)	909	WP_013013236.1	manganese catalase family protein	-
MRUB_RS04830 (Mrub_0953)	-	942289..943218 (+)	930	WP_013013237.1	hydrogen peroxide-inducible genes activator	-

Sequence

Protein

• Length: 885 a.a.
• Molecular weight: 97896.59 Da
• Isoelectric Point: 5.0228

>NTDB_id=36677 MRUB_RS04810 WP_013013233.1 935623..938280(+) (pilF) [Meiothermus ruber DSM 1279]
MANVLTIGDKRLGAALLDMGLLGDEELQRALEQHREVGGNLSEIIVDLGLLSERRIAQAIEQAFGIPLVELADMEISPEA
KSLIPAERARDLGAIPFSVEGGTLRVALLNPLDNLVLEELEDLTNQIIEPYLATPSSFRYALAVHYPELGLPVPPPPSAA
SQGEMQLGEILVSKGWLSREDLEAALVEQEKTGELLGRLLVNKFGLAEERLYQALAEQAGIEFRAELDDLELQQEVSAYL
LRPDALRYQAIPARQDGQQVLVVLADPRHRHAVARLIERPTKFILTLPKVWEALFGKAYPEKSRLGEALVQEGKLGRAAL
QDALSVQRRMGKTRPLGEVLVELGYVSAQDVEEALAKQRQGGGRLEDTLVQSGKIKPEMLAKSLATQLGYPYIDPTDSPP
DPSVLTMVPESTVRRYTIFPHHLEGNTLVVLMKDPRNILAIDDLRMITKRDVMPAVSAEAAITKLIERFYGSTTGVEELA
REFEGKKREEEAADTSALDDNAVVKLVNSIIREAFLQDASDIHIEPRQSDILVRIRIDGTLREYMRLPRGAGPAIASRIK
IMANLDIAERRLPQDGRVRYRDRSIDLDLRLSTLPTVYGEKIVMRLLRKAADIPEIEQLGFAPDVFQRFEDVISKPYGIF
LITGPTGSGKSFTTFSILKRIATPDKNTTTIEDPVEYEIPGINQTQVNPVAGLTFAKALRSFLRQDPDIIMVGEIRDSET
AKIATEAALTGHLVIATLHTNDAAGAVTRLDEMGVELFNISAALVGVLAQRLVRKICEHCKIQVEPDPTVLRRLELSREE
LRGKTLYKGTGCDKCNGTGYKGRTAIHELMVLDDEIRRAIVEGQSATQIKEIARRGGMKTLREDGIQKAFLGLTTLEEVM
ARTNE

Nucleotide

• Length: 2658 bp

>NTDB_id=36677 MRUB_RS04810 WP_013013233.1 935623..938280(+) (pilF) [Meiothermus ruber DSM 1279]
ATGGCCAACGTCTTGACAATTGGAGATAAACGGCTGGGCGCTGCCCTGTTGGACATGGGGCTGCTGGGGGATGAAGAACT
GCAGCGAGCCCTCGAGCAGCACCGTGAAGTGGGGGGGAACCTCTCGGAGATTATCGTCGACCTGGGCTTGCTCTCTGAGC
GACGCATTGCCCAGGCCATTGAACAGGCCTTTGGTATTCCGCTGGTGGAGCTAGCCGACATGGAAATTTCACCCGAGGCC
AAGTCGCTCATTCCGGCAGAGCGGGCGCGGGATCTGGGTGCCATTCCCTTCAGCGTGGAGGGGGGCACCTTGCGGGTGGC
CCTGCTAAACCCGTTGGACAACCTGGTGCTTGAGGAGCTCGAAGACCTCACCAATCAGATCATCGAGCCCTACCTGGCTA
CCCCGTCCTCTTTTCGCTACGCCCTGGCGGTGCACTACCCCGAGCTGGGCCTGCCGGTGCCCCCGCCGCCCTCGGCTGCC
TCGCAGGGCGAGATGCAACTGGGTGAAATCCTGGTCAGCAAAGGCTGGCTGAGCCGGGAGGATCTCGAGGCCGCTTTGGT
TGAGCAGGAGAAGACCGGTGAGCTATTGGGTCGGCTGCTTGTGAACAAATTCGGCCTGGCCGAGGAACGGCTTTACCAGG
CCCTGGCCGAGCAGGCCGGTATCGAGTTTAGGGCGGAACTGGACGACCTCGAGCTGCAACAGGAGGTCTCGGCCTATTTG
CTGCGCCCCGATGCGCTGCGCTACCAGGCTATACCAGCCCGTCAGGATGGGCAGCAGGTGCTGGTGGTACTGGCCGACCC
ACGCCACCGACACGCTGTGGCCCGGTTGATCGAACGCCCCACCAAGTTCATCCTGACCCTGCCCAAGGTTTGGGAAGCCT
TGTTTGGCAAAGCCTATCCTGAAAAGAGCCGCCTGGGGGAAGCCTTGGTGCAGGAAGGTAAGCTGGGACGCGCCGCTTTA
CAGGATGCGCTTTCGGTGCAGCGCCGCATGGGCAAAACCCGGCCTCTGGGCGAAGTGTTGGTCGAACTGGGTTACGTGAG
CGCCCAGGATGTGGAAGAGGCCCTGGCCAAACAGCGGCAGGGCGGGGGCCGCCTCGAAGACACCCTGGTGCAGTCGGGCA
AGATCAAGCCAGAGATGCTGGCGAAGAGCCTGGCCACCCAGCTTGGCTACCCCTACATCGACCCCACCGACTCGCCCCCC
GATCCTTCGGTGCTCACCATGGTGCCCGAAAGCACGGTTCGGCGCTACACCATTTTTCCCCACCACCTCGAGGGCAACAC
CCTGGTGGTCTTGATGAAAGACCCCCGCAATATCCTGGCCATCGACGACCTACGCATGATCACCAAACGCGATGTGATGC
CGGCGGTCTCAGCGGAAGCGGCCATTACCAAGCTTATCGAGCGGTTCTATGGCAGCACCACCGGGGTGGAGGAGCTGGCC
CGGGAGTTTGAGGGTAAGAAAAGAGAAGAAGAGGCTGCCGATACCAGCGCCCTCGACGATAACGCGGTGGTCAAGCTGGT
CAACAGCATTATCCGCGAGGCCTTCTTGCAGGATGCTTCGGACATCCACATCGAGCCCCGCCAGTCGGATATCCTGGTGC
GCATCCGAATCGATGGCACCCTGCGGGAGTACATGCGCCTGCCCAGGGGGGCCGGGCCGGCGATTGCCTCGAGGATCAAG
ATTATGGCCAACCTGGACATCGCCGAGCGACGCCTGCCCCAGGACGGCCGGGTGCGCTACCGCGACCGTTCCATTGACCT
GGATCTGCGTCTTTCCACCCTGCCCACCGTATACGGCGAAAAAATTGTAATGCGTCTGCTGCGCAAAGCCGCCGACATCC
CTGAAATTGAGCAGCTCGGATTCGCGCCGGATGTGTTTCAGCGCTTTGAAGACGTGATTTCCAAGCCCTACGGCATCTTC
CTGATTACCGGGCCTACGGGCTCGGGTAAGTCCTTCACCACCTTCAGCATTCTGAAGCGCATCGCCACCCCCGACAAAAA
CACCACCACCATCGAAGACCCGGTCGAGTACGAGATCCCCGGGATTAACCAAACCCAGGTGAACCCGGTGGCCGGCCTGA
CCTTTGCCAAGGCCCTGCGGAGCTTCCTGCGGCAAGACCCCGATATCATCATGGTGGGTGAGATCCGCGACTCTGAAACG
GCCAAGATTGCCACCGAGGCGGCCCTGACCGGTCACCTGGTGATCGCCACCCTGCACACCAACGATGCCGCAGGGGCCGT
TACCCGGCTGGATGAGATGGGGGTGGAACTCTTCAACATCTCGGCGGCGCTGGTGGGGGTGCTGGCCCAGCGCTTGGTGC
GCAAGATCTGCGAGCACTGCAAAATTCAGGTTGAGCCCGACCCAACCGTGCTACGCCGGCTGGAGCTGTCCAGGGAGGAG
CTGCGTGGCAAAACCCTCTACAAAGGCACGGGGTGCGATAAGTGCAACGGCACCGGCTATAAGGGCCGTACGGCCATCCA
CGAGCTCATGGTGCTCGACGATGAGATCCGCCGGGCTATTGTGGAGGGGCAGTCGGCCACCCAGATTAAGGAGATCGCCC
GCAGGGGTGGTATGAAAACCCTGCGCGAGGACGGTATTCAAAAAGCTTTCCTGGGCCTGACCACCCTGGAAGAGGTGATG
GCCCGCACCAACGAGTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (509-775)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-147)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (173-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-473)

3D structure

• AlphaFold DB: D3PQI1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	69.775	100	0.702
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.674	99.887	0.566