Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FED52_RS00450 Genome accession   NZ_CP040676
Coordinates   19459..20835 (+) Length   458 a.a.
NCBI ID   WP_034781742.1    Uniprot ID   A0A4R0Z2X9
Organism   Exiguobacterium mexicanum strain A-EM     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 14459..25835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FED52_RS00430 - 14980..15447 (+) 468 WP_114166644.1 CtsR family transcriptional regulator -
  FED52_RS13945 - 15692..15901 (+) 210 WP_240731275.1 UvrB/UvrC motif-containing protein -
  FED52_RS00440 - 15901..16944 (+) 1044 WP_205729346.1 protein arginine kinase -
  FED52_RS00445 clpC 16969..19404 (+) 2436 WP_138858553.1 ATP-dependent protease ATP-binding subunit ClpC -
  FED52_RS00450 radA 19459..20835 (+) 1377 WP_034781742.1 DNA repair protein RadA Machinery gene
  FED52_RS00455 - 20951..22015 (+) 1065 WP_034781740.1 PIN/TRAM domain-containing protein -
  FED52_RS00460 ispD 22075..22752 (+) 678 WP_138858554.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  FED52_RS00465 ispF 22749..23222 (+) 474 WP_021066582.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  FED52_RS00470 gltX 23268..24725 (+) 1458 WP_205729365.1 glutamate--tRNA ligase -
  FED52_RS00475 cysE 24975..25664 (+) 690 WP_138858556.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49871.39 Da        Isoelectric Point: 6.3235

>NTDB_id=366406 FED52_RS00450 WP_034781742.1 19459..20835(+) (radA) [Exiguobacterium mexicanum strain A-EM]
MAKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPERLSDVVSQEETRVHTNSQEF
DRVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGRKVLYISGEESLKQTKLRAERLGLPTEDLYVLAETDMLMIE
RVIDEEEPGFVIIDSIQTVYMDEIQSAPGSVAQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMKEGGLEEVLNPSEIFLEERTAGVSGSTVVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNLGGWTAPPGIDVVGVSTVDEALQLAIPF

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=366406 FED52_RS00450 WP_034781742.1 19459..20835(+) (radA) [Exiguobacterium mexicanum strain A-EM]
ATGGCAAAAATTAAAACGAAATACGTATGTCAGTCATGCGGGTACGAATCCGCAAAATGGATGGGGCGTTGCCCAGGATG
TAATGAATGGAACACACTCGTTGAAGAGTTTGTCGAAGAGAAAAAGGCAAAGCGGGGGGCCGCCTTCGTTCATAGCTCCT
CGAGACAGCTGAAGCCGGAGCGTCTGTCGGACGTTGTGTCACAAGAAGAGACGCGGGTCCATACGAACAGTCAAGAGTTT
GACCGTGTGCTCGGGGGCGGAATCGTTCCAGGCTCACTCGTCCTCGTCGGTGGCGATCCGGGAATCGGAAAGTCGACGAT
TTTGCTTCAAGTGAGCGCCCATTTGGCGCACAGTGGACGGAAAGTGCTCTATATTTCCGGGGAAGAATCGCTCAAACAGA
CGAAACTTCGCGCCGAGCGGCTTGGCCTCCCGACCGAGGACTTATATGTCCTCGCGGAGACGGATATGTTGATGATTGAA
CGGGTCATCGATGAAGAGGAACCAGGGTTCGTCATTATCGACTCGATTCAAACCGTCTATATGGATGAGATCCAGTCGGC
GCCAGGTAGTGTCGCCCAAGTTCGGGAATGTACGGCAGCGCTCATGAAAATCGCCAAGACCCGTGGAATTGCCGTCTTTA
TCGTCGGTCACGTGACGAAACAAGGTTCGATTGCGGGACCTCGTCTGCTCGAGCATATGGTCGATGCCGTCCTTTACTTT
GAAGGCGAACGTCACCACACGTTCCGTATTTTACGGGCCGTCAAAAATCGGTTCGGATCGACGAACGAGATCGGTATCTT
TGAAATGAAGGAAGGCGGTCTCGAAGAAGTGCTCAATCCGTCTGAAATTTTCCTAGAAGAACGGACTGCCGGTGTCTCGG
GCTCAACTGTCGTCGCGTCGATGGAAGGGACGCGGACGGTGCTCGTCGAGCTGCAGGCATTAATTTCCCCGACCTCATTC
GGAAATCCACGTCGGATGGCAACAGGGATTGACCAGAACCGGGTCGCCCTTCTCATGGCTGTGCTCGAGAAGCGTTCGGG
TCTCTTGCTCCAAACGCAAGACGCTTATTTGAAGGCGGCCGGGGGCGTTAAATTGGACGAACCGGCCATCGACTTGGCCA
TTTGTATTTCGATCGCATCAAGCTTCCGGGACCGTCCGACCCGTCCGACGGATGTATGTATCGGCGAAGTCGGATTGACC
GGTGAGGTACGGCGGGTGTCACGGATTGAGCAACGTGTCGCTGAAGCGGCCAAGCTTGGTTTCACCCGGGCCATCATTCC
GAAAAACAACCTCGGGGGCTGGACAGCCCCGCCTGGTATCGATGTCGTCGGTGTCAGTACTGTCGATGAAGCGCTCCAAC
TCGCTATCCCGTTCTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R0Z2X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.465

99.563

0.731
  radA Streptococcus mitis NCTC 12261

61.81

98.908

0.611
  radA Streptococcus pneumoniae Rx1

61.81

98.908

0.611
  radA Streptococcus pneumoniae D39

61.81

98.908

0.611
  radA Streptococcus pneumoniae R6

61.81

98.908

0.611
  radA Streptococcus pneumoniae TIGR4

61.81

98.908

0.611
  radA Streptococcus mitis SK321

61.81

98.908

0.611

Multiple sequence alignment