Detailed information NTG0366226

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: FFX42_RS00305
• Genome accession: NZ_CP040665
• Coordinates: 63498..64847 (-)
• Length: 449 a.a.
• NCBI ID: WP_000815646.1
• Uniprot ID: Q9XB96
• Organism: Staphylococcus aureus strain D592
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	43518..64847	63498..64847	within	0

Gene organization within MGE regions

Location: 43518..64847

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FFX42_RS00190 (FFX42_00190)	mecA	43518..45527 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
FFX42_RS00195 (FFX42_00195)	mecR1	45624..47381 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
FFX42_RS00200 (FFX42_00200)	mecI	47381..47752 (+)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
FFX42_RS00205 (FFX42_00205)	psm-mec	47837..47905 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
FFX42_RS00210 (FFX42_00210)	-	48239..49369 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
FFX42_RS00215 (FFX42_00215)	cstB	49483..50817 (-)	1335	Protein_39	persulfide dioxygenase-sulfurtransferase CstB	-
FFX42_RS00220 (FFX42_00220)	-	50850..51914 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
FFX42_RS00225 (FFX42_00225)	cstR	52050..52310 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
FFX42_RS00230 (FFX42_00230)	-	52310..52948 (+)	639	Protein_42	sulfite exporter TauE/SafE family protein	-
FFX42_RS00240 (FFX42_00240)	-	53293..53955 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
FFX42_RS14705 (FFX42_00250)	-	54347..54406 (+)	60	WP_010959190.1	erythromycin resistance leader peptide	-
FFX42_RS00255 (FFX42_00255)	erm(A)	54453..55184 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
FFX42_RS00260 (FFX42_00260)	-	55310..56092 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
FFX42_RS00265 (FFX42_00265)	-	56243..56620 (-)	378	WP_000361059.1	DUF6262 family protein	-
FFX42_RS00270 (FFX42_00270)	-	56627..58519 (-)	1893	WP_001557544.1	site-specific integrase	-
FFX42_RS00275 (FFX42_00275)	-	58516..59601 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
FFX42_RS00280 (FFX42_00280)	-	59720..60037 (-)	318	WP_000659050.1	JAB domain-containing protein	-
FFX42_RS00285 (FFX42_00285)	-	60058..60564 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
FFX42_RS00290 (FFX42_00290)	-	60582..60893 (-)	312	WP_000700853.1	DUF960 family protein	-
FFX42_RS00295 (FFX42_00295)	-	60980..61330 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
FFX42_RS00300 (FFX42_00300)	ccrB	61848..63476 (-)	1629	WP_001186602.1	cassette chromosome recombinase CcrB	Machinery gene
FFX42_RS00305 (FFX42_00305)	ccrA	63498..64847 (-)	1350	WP_000815646.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52607.20 Da
• Isoelectric Point: 9.9585

>NTDB_id=366226 FFX42_RS00305 WP_000815646.1 63498..64847(-) (ccrA) [Staphylococcus aureus strain D592]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=366226 FFX42_RS00305 WP_000815646.1 63498..64847(-) (ccrA) [Staphylococcus aureus strain D592]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-322)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9XB96

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	100	100	1
ccrA	Staphylococcus aureus COL	74.833	100	0.748