Detailed information    

insolico Bioinformatically predicted

Overview

Name   vraS   Type   Regulator
Locus tag   FF960_RS10520 Genome accession   NZ_CP040625
Coordinates   2068203..2069246 (-) Length   347 a.a.
NCBI ID   WP_001017131.1    Uniprot ID   A0A0D1IMN4
Organism   Staphylococcus aureus strain JKD6004-DR     
Function   repress expression of competence genes (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2060295..2102164 2068203..2069246 within 0


Gene organization within MGE regions


Location: 2060295..2102164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FF960_RS10475 (FF960_10490) - 2060505..2061551 (+) 1047 WP_001145732.1 tyrosine-type recombinase/integrase -
  FF960_RS10480 (FF960_10495) yfkAB 2061596..2062741 (+) 1146 WP_001245448.1 radical SAM/CxCxxxxC motif protein YfkAB -
  FF960_RS10485 (FF960_10500) - 2063001..2063531 (-) 531 WP_095344333.1 acyl-CoA thioesterase -
  FF960_RS10490 (FF960_10505) - 2063621..2064877 (-) 1257 WP_001795438.1 aminopeptidase -
  FF960_RS10495 (FF960_10510) - 2064880..2065080 (-) 201 WP_000180461.1 DUF1128 family protein -
  FF960_RS10500 (FF960_10515) - 2065227..2065691 (+) 465 WP_000228666.1 low molecular weight protein-tyrosine-phosphatase -
  FF960_RS10505 (FF960_10520) - 2065698..2065973 (+) 276 WP_000428172.1 YtxH domain-containing protein -
  FF960_RS10510 (FF960_10525) - 2066251..2067468 (+) 1218 WP_000037075.1 YihY/virulence factor BrkB family protein -
  FF960_RS10515 (FF960_10530) vraR 2067584..2068213 (-) 630 WP_000153530.1 two-component system response regulator VraR Regulator
  FF960_RS10520 (FF960_10535) vraS 2068203..2069246 (-) 1044 WP_001017131.1 sensor histidine kinase Regulator
  FF960_RS10525 (FF960_10540) liaF 2069243..2069944 (-) 702 WP_000149064.1 cell wall-active antibiotics response protein LiaF -
  FF960_RS10530 (FF960_10545) - 2069959..2070345 (-) 387 WP_001110179.1 hypothetical protein -
  FF960_RS10535 (FF960_10550) map 2070562..2071320 (-) 759 WP_000636142.1 type I methionyl aminopeptidase -
  FF960_RS10540 (FF960_10555) - 2071907..2072893 (+) 987 WP_000999713.1 aromatic acid exporter family protein -
  FF960_RS10545 (FF960_10560) - 2073324..2073737 (-) 414 WP_001549145.1 hypothetical protein -
  FF960_RS10550 (FF960_10565) - 2073766..2073876 (-) 111 WP_001790257.1 hypothetical protein -
  FF960_RS10555 (FF960_10570) - 2073962..2074693 (-) 732 WP_000544967.1 type 1 glutamine amidotransferase -
  FF960_RS10560 (FF960_10575) murT 2074695..2076008 (-) 1314 WP_001250336.1 lipid II isoglutaminyl synthase subunit MurT -
  FF960_RS10565 (FF960_10580) ftnA 2076300..2076800 (+) 501 WP_000949467.1 H-type ferritin FtnA -
  FF960_RS10570 (FF960_10585) - 2077159..2077713 (+) 555 WP_000613733.1 3'-5' exonuclease -
  FF960_RS10575 (FF960_10590) dinB 2077781..2078851 (-) 1071 WP_000140176.1 DNA polymerase IV -
  FF960_RS10580 (FF960_10595) - 2079098..2079628 (-) 531 WP_000548781.1 DUF3267 domain-containing protein -
  FF960_RS10585 (FF960_10600) rlmD 2079798..2081159 (-) 1362 WP_001147868.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  FF960_RS10590 (FF960_10605) - 2081240..2082187 (-) 948 WP_001231451.1 diacylglycerol kinase -
  FF960_RS10595 (FF960_10610) - 2082770..2082916 (-) 147 WP_001797372.1 hypothetical protein -
  FF960_RS10600 (FF960_10615) gatB 2082956..2084383 (-) 1428 WP_000545370.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  FF960_RS10605 (FF960_10620) gatA 2084396..2085853 (-) 1458 WP_000027928.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA -
  FF960_RS10610 (FF960_10625) gatC 2085855..2086157 (-) 303 WP_000170162.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  FF960_RS10615 (FF960_10630) putP 2086524..2088062 (+) 1539 WP_000957015.1 sodium/proline symporter PutP -
  FF960_RS10620 (FF960_10635) - 2088151..2089350 (-) 1200 WP_000831698.1 CamS family sex pheromone protein -
  FF960_RS10625 (FF960_10640) ligA 2089363..2091366 (-) 2004 WP_000774565.1 NAD-dependent DNA ligase LigA -
  FF960_RS10630 (FF960_10645) pcrA 2091370..2093562 (-) 2193 WP_000992921.1 DNA helicase PcrA -
  FF960_RS10635 (FF960_10650) - 2093559..2094251 (-) 693 WP_000272068.1 heptaprenylglyceryl phosphate synthase -
  FF960_RS10640 (FF960_10655) - 2094423..2094725 (-) 303 WP_001165363.1 YerC/YecD family TrpR-related protein -
  FF960_RS10645 (FF960_10660) purB 2094834..2096129 (-) 1296 WP_000572878.1 adenylosuccinate lyase -
  FF960_RS10655 (FF960_10670) scpA 2096990..2098156 (+) 1167 WP_000827748.1 cysteine protease staphopain A -
  FF960_RS10660 (FF960_10675) - 2098187..2098513 (+) 327 WP_000434933.1 staphostatin A -
  FF960_RS10665 (FF960_10680) - 2098805..2098978 (-) 174 WP_000669862.1 NETI motif-containing protein -
  FF960_RS10670 (FF960_10685) - 2098959..2099561 (-) 603 WP_000011542.1 DUF2179 domain-containing protein -
  FF960_RS10675 (FF960_10690) nadE 2099832..2100653 (-) 822 WP_000040873.1 ammonia-dependent NAD(+) synthetase -
  FF960_RS10680 (FF960_10695) - 2100646..2102115 (-) 1470 WP_000284431.1 nicotinate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 40044.52 Da        Isoelectric Point: 6.0577

>NTDB_id=365966 FF960_RS10520 WP_001017131.1 2068203..2069246(-) (vraS) [Staphylococcus aureus strain JKD6004-DR]
MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIFGIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKT
QIERSMEGETVGINDQNIEIYSETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLGEGIKDLVIDLQKKVPMKVVHEI
QDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVELFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGAT
FHIVSLPDSGTRIEVKAPLNKEDSYDD

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=365966 FF960_RS10520 WP_001017131.1 2068203..2069246(-) (vraS) [Staphylococcus aureus strain JKD6004-DR]
ATGAACCACTACATTAGAACAATTGGTTCAATGCTCATCTTAGTATATAGCATGCTAGCTGCATTTCTGTTCATCGATAA
AGTTTTTGTAAATATCATCTATTTTCAAGGTATGTTTTATACACAAATATTCGGAATACCAGTTTTTTTATTTTTAAATC
TCATCATCATATTATTGTGTATTATTGTTGGTTCGGTACTCGCTTACAAAATCAATCAGCAAAATGATTGGATTAAGACG
CAAATTGAGCGTTCAATGGAAGGCGAAACAGTTGGCATTAATGATCAAAATATAGAAATATATAGTGAAACGTTAGATTT
ATACCATACACTCGTACCTTTAAATCAAGAATTGCATAAGTTGCGACTTAAAACTCAAAACTTAACCAATGAAAATTATA
ATATTAATGATGTGAAAGTTAAAAAGATTATTGAAGATGAACGTCAAAGACTAGCACGAGAACTTCACGATTCTGTTAGT
CAGCAACTTTTTGCGGCAAGTATGATGCTATCTGCTATCAAAGAAACGAAGTTAGAACCACCATTAGACCAACAAATTCC
TATTTTAGAGAAAATGGTTCAAGATTCGCAGTTAGAAATGCGTGCTTTGCTGTTACATTTAAGACCGCTTGGTTTAAAAG
ACAAATCTTTAGGTGAGGGTATTAAAGATTTAGTTATTGATTTACAAAAAAAAGTGCCAATGAAAGTTGTGCATGAAATA
CAAGATTTTAAAGTGCCTAAAGGTATTGAAGATCATTTGTTCAGAATTACACAGGAAGCAATTTCGAATACATTGCGTCA
TTCAAACGGTACAAAAGTGACAGTAGAATTGTTTAATAAAGACGATTATTTATTGTTGAGAATTCAAGATAATGGTAAAG
GTTTTAATGTTGATGAAAAATTAGAACAAAGTTATGGACTTAAAAATATGCGTGAAAGAGCTTTGGAAATTGGTGCAACG
TTCCATATTGTATCATTGCCAGATTCAGGTACACGTATCGAGGTGAAAGCACCTTTAAATAAGGAGGATTCGTATGACGA
TTAA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(249-340)

Histidine kinase (HisKA_3)

(147-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1IMN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  vraS Staphylococcus aureus N315

100

100

1

Multiple sequence alignment