Detailed information NTG0365933

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: FF960_RS00320
• Genome accession: NZ_CP040625
• Coordinates: 71476..72822 (-)
• Length: 448 a.a.
• NCBI ID: WP_000816499.1
• Uniprot ID: Q9R3W9
• Organism: Staphylococcus aureus strain JKD6004-DR
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	51663..72822	71476..72822	within	0

Gene organization within MGE regions

Location: 51663..72822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FF960_RS00210 (FF960_00210)	mecA	51663..53672 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
FF960_RS00215 (FF960_00215)	mecR1	53769..55526 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
FF960_RS00220 (FF960_00220)	mecI	55526..55897 (+)	372	WP_130828128.1	mecA-type methicillin resistance repressor MecI	-
FF960_RS00225 (FF960_00225)	psm-mec	55982..56050 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
FF960_RS00230 (FF960_00230)	-	56384..57514 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
FF960_RS00235 (FF960_00235)	cstB	57628..58962 (-)	1335	Protein_41	persulfide dioxygenase-sulfurtransferase CstB	-
FF960_RS00240 (FF960_00240)	-	58995..60059 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
FF960_RS00245 (FF960_00245)	cstR	60195..60455 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
FF960_RS00250 (FF960_00250)	-	60455..61093 (+)	639	Protein_44	sulfite exporter TauE/SafE family protein	-
FF960_RS00260 (FF960_00260)	-	61367..61983 (-)	617	Protein_45	CadD family cadmium resistance transporter	-
FF960_RS00265 (FF960_00265)	-	62064..64478 (-)	2415	WP_116100491.1	cadmium-translocating P-type ATPase CadA	-
FF960_RS00270 (FF960_00270)	-	64471..64836 (-)	366	WP_000159127.1	metalloregulator ArsR/SmtB family transcription factor	-
FF960_RS00275 (FF960_00275)	-	65074..65451 (-)	378	WP_001041908.1	DUF6262 family protein	-
FF960_RS00280 (FF960_00280)	-	65458..67350 (-)	1893	WP_002485298.1	site-specific integrase	-
FF960_RS00285 (FF960_00285)	-	67546..67869 (-)	324	WP_000088091.1	JAB domain-containing protein	-
FF960_RS00290 (FF960_00290)	-	67862..68068 (-)	207	WP_001077283.1	hypothetical protein	-
FF960_RS00295 (FF960_00295)	-	68070..68591 (-)	522	WP_000836008.1	DUF1643 domain-containing protein	-
FF960_RS00300 (FF960_00300)	-	68610..68921 (-)	312	WP_000833212.1	DUF960 family protein	-
FF960_RS00310 (FF960_00310)	-	69006..69356 (-)	351	WP_000171449.1	SAUGI family uracil-DNA glycosylase inhibitor	-
FF960_RS00315 (FF960_00315)	ccrB	69827..71455 (-)	1629	WP_001186596.1	cassette chromosome recombinase CcrB	Machinery gene
FF960_RS00320 (FF960_00320)	ccrA	71476..72822 (-)	1347	WP_000816499.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 448 a.a.
• Molecular weight: 52138.19 Da
• Isoelectric Point: 10.1760

>NTDB_id=365933 FF960_RS00320 WP_000816499.1 71476..72822(-) (ccrA) [Staphylococcus aureus strain JKD6004-DR]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide

• Length: 1347 bp

>NTDB_id=365933 FF960_RS00320 WP_000816499.1 71476..72822(-) (ccrA) [Staphylococcus aureus strain JKD6004-DR]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (168-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: Q9R3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus COL	78.174	100	0.783
ccrA	Staphylococcus aureus N315	72.383	100	0.725