Detailed information NTG0365340

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: E3S76_RS05440
• Genome accession: NZ_CP047849
• Coordinates: 1100636..1104289 (-)
• Length: 1217 a.a.
• NCBI ID: WP_160203732.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain UP_403
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1095636..1109289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3S76_RS05415 (E3S76_05415)	-	1096065..1096373 (-)	309	WP_000390575.1	metal-sulfur cluster assembly factor	-
E3S76_RS05420 (E3S76_05420)	-	1096487..1097311 (+)	825	WP_001182436.1	Cof-type HAD-IIB family hydrolase	-
E3S76_RS05425 (E3S76_05425)	-	1097363..1098679 (+)	1317	WP_001124525.1	CoA-disulfide reductase	-
E3S76_RS05430 (E3S76_05430)	-	1098850..1099239 (-)	390	WP_000902817.1	YisL family protein	-
E3S76_RS05435 (E3S76_05435)	-	1099568..1100470 (-)	903	WP_000670752.1	fumarylacetoacetate hydrolase family protein	-
E3S76_RS05440 (E3S76_05440)	addA	1100636..1104289 (-)	3654	WP_160203732.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
E3S76_RS05445 (E3S76_05445)	addB	1104290..1107766 (-)	3477	WP_000172342.1	helicase-exonuclease AddAB subunit AddB	-
E3S76_RS05450 (E3S76_05450)	lepB	1107926..1108501 (-)	576	WP_000711486.1	signal peptidase I	-
E3S76_RS05455 (E3S76_05455)	lepB	1108517..1109041 (-)	525	WP_000758215.1	signal peptidase I	-

Sequence

Protein

• Length: 1217 a.a.
• Molecular weight: 141232.77 Da
• Isoelectric Point: 4.9495

>NTDB_id=365340 E3S76_RS05440 WP_160203732.1 1100636..1104289(-) (addA) [Staphylococcus aureus strain UP_403]
MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRI
QEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKAYDLFS
MMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSD
YFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILV
DEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRKEVLS
TTNYIFKHMMDEQVGEVKYDEAAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMK
TGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQF
KEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIG
GRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKD
FNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLAYVALTRAKEQLYLIGRVKNDKSLLE
LEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEY
RSVNQLETMQNGNEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQ
GKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQVYRELP
FVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILN
KEVKGYLYFFKFGTLQL

Nucleotide

• Length: 3654 bp

>NTDB_id=365340 E3S76_RS05440 WP_160203732.1 1100636..1104289(-) (addA) [Staphylococcus aureus strain UP_403]
ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATTTACGCAACTGGACAAGATGT
ACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTACTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCA
TTGATGTCGATCGACTTTTAGTCGTAACGTTTACAAACTTAAGCGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATTCATCAAGCACAAATATCTAC
ACTCCATAGTTTTTGCTTGAAATTAATTCAACAGCATTATGATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTG
AAGCTGAAAATATTTTATTATTAGAACAAACGATAGATGAGGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
ATTGAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAG
CGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTA
TTCAACTACTAACAGACTTATCTAAAGTATTTATCACAGCTGCTTATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
ATGATGGATAGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGGCGTGTTTTAGAAGGTGGCTT
TATTGATATACCTTATTTAACTGGTCACGAATTTGGCGCGCGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATG
AAATGATGGTCGATGCCTTAGAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAGAGTGAT
TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTAAAGTACCTTGCGCGTATTGT
GAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGCAAAAATATTTTGGATTTTTCTGATTATGAACATTTTGCAT
TACAAATTTTAACTAATGAGGATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTCCAAGAAATATTGGTC
GATGAGTATCAAGATACGAACCGAGTTCAAGAGAAAATACTATCTTGCATCAAAACGGGTGATGAACATAATGGTAATTT
ATTTATGGTTGGAGATGTTAAGCAATCCATTTATAAATTTAGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAAC
GCTTTACTATAGATGGAGATGGCACTGGACGTCGAATTGATTTGTCGCAAAACTTCCGTTCTCGAAAAGAAGTACTGTCA
ACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAAGCGGCACAGTTGTATTA
TGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTCCTTGTTGAAGCGGATCAAGAACATAGTGATTTAA
CTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAA
ACAGGAAGCTATAGAAGTGCGACATACAAGGATATCGTTATTCTAGAACGCAGCTTTGGACAAGCTCGCAATTTACAACA
AGCCTTTAAAAATGAAGATATTCCATTCCATGTGAATAGTCGTGAAGGTTACTTTGAACAAACAGAAGTCCGCTTAGTAT
TATCATTTTTAAGAGCGATAGATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGCTCCGTTATATATCAGTTC
AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAATCGATTGTAAATTACATTAA
TGACGAAGCAGCAGATGCAATTTTAGTTGATAAATTAAAAATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTA
AAGATCATCCGGTGTATCAGTTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
GGACGTGGACGACGTGCAAATCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAATTCAAGTTTTAGAGGTTTATA
TCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGCAAAGATTTTGGTGAGGAAAATGTAGTTGGTCCAAACGATA
ATGTCGTTAGAATGATGACAATTCATAGTAGTAAAGGTCTAGAGTTTCCATTTGTCATTTATTCTGGATTGTCAAAAGAT
TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATGGATTATTTTGATGTGGATAA
AGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAGAGCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGAT
TAGCCTATGTAGCATTAACAAGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCATTACTAGAA
CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCAAATCCGTTCCATCTTATTTA
TAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGATGATTTAAAATTTGAAAAAGATATAGCACAAATTGAAGATA
GTAGTCGTCCGAATGTAAATATTTCAATTGTGTACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATGATGAATAT
CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAAGATGTTAAAGCACAAATTAAACACCAACTTGATTATCG
ATATCCATATGTAAATGATACTAAAAAGCCCTCAAAACAATCTGTTTCTGAATTGAAAAGACAATATGAAACAGAAGAAA
GTGGCACAAGTTACGAACGAGTAAGGCAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCATTCAAAAAAGAACGCATATC
TGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGATAAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTA
TGGATGAAATAATGACATTTATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGTCTCAATTATTCAAGGTATGATTGACTT
AATCTTTGTTAAAGATGGTGTGCATTATTTTGTAGACTATAAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATG
AAGAAATTGGTACACAATTAAAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATTCTTAAT
AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTATAG

Domains

Predicted by InterProScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1040-1210)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (12-455)

• UvrD-like helicase C-terminal domain (UvrD_C) (466-869)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	36.551	100	0.38