Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   FC605_RS12955 Genome accession   NZ_CP040528
Coordinates   2516061..2516678 (-) Length   205 a.a.
NCBI ID   WP_080529560.1    Uniprot ID   -
Organism   Bacillus subtilis strain PR10     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2511061..2521678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FC605_RS12925 (FC605_12925) rpsT 2511363..2511629 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  FC605_RS12930 (FC605_12930) holA 2511644..2512687 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  FC605_RS12935 (FC605_12935) - 2512727..2512876 (-) 150 WP_003229985.1 hypothetical protein -
  FC605_RS12940 (FC605_12940) yqzM 2512917..2513051 (+) 135 WP_003229983.1 YqzM family protein -
  FC605_RS12945 (FC605_12945) comEC 2513091..2515421 (-) 2331 WP_080529559.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  FC605_RS12950 (FC605_12950) comEB 2515425..2515994 (-) 570 WP_003229978.1 ComE operon protein 2 -
  FC605_RS12955 (FC605_12955) comEA 2516061..2516678 (-) 618 WP_080529560.1 competence protein ComEA Machinery gene
  FC605_RS12960 (FC605_12960) comER 2516762..2517583 (+) 822 WP_014480313.1 late competence protein ComER -
  FC605_RS12965 (FC605_12965) yqeM 2517649..2518392 (-) 744 WP_029317885.1 class I SAM-dependent methyltransferase -
  FC605_RS12970 (FC605_12970) rsfS 2518389..2518745 (-) 357 WP_014480315.1 ribosome silencing factor -
  FC605_RS12975 (FC605_12975) yqeK 2518763..2519323 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  FC605_RS12980 (FC605_12980) nadD 2519313..2519882 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  FC605_RS12985 (FC605_12985) yhbY 2519894..2520184 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  FC605_RS12990 (FC605_12990) aroE 2520178..2521020 (-) 843 WP_080529561.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21770.57 Da        Isoelectric Point: 4.9802

>NTDB_id=365029 FC605_RS12955 WP_080529560.1 2516061..2516678(-) (comEA) [Bacillus subtilis strain PR10]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESSETIVIDIKGAVKHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREESGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=365029 FC605_RS12955 WP_080529560.1 2516061..2516678(-) (comEA) [Bacillus subtilis strain PR10]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCCTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTAAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGAATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAGCGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

35.909

100

0.385

Multiple sequence alignment