Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FE240_RS14370 Genome accession   NZ_CP040449
Coordinates   3025421..3026860 (-) Length   479 a.a.
NCBI ID   WP_193001878.1    Uniprot ID   A0A5J6WZS2
Organism   Aeromonas simiae strain A6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3020421..3031860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE240_RS14340 (FE240_14345) - 3020864..3022237 (+) 1374 WP_193001872.1 Do family serine endopeptidase -
  FE240_RS14345 (FE240_14350) degS 3022338..3023423 (+) 1086 WP_042045436.1 outer membrane-stress sensor serine endopeptidase DegS -
  FE240_RS14350 (FE240_14355) npr 3023481..3023753 (-) 273 WP_193001874.1 PTS phosphocarrier protein NPr -
  FE240_RS14355 (FE240_14360) rapZ 3023750..3024616 (-) 867 WP_193001876.1 RNase adapter RapZ -
  FE240_RS14360 (FE240_14365) ptsN 3024664..3025110 (-) 447 WP_042045433.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FE240_RS14365 (FE240_14370) hpf 3025112..3025399 (-) 288 WP_042045432.1 ribosome hibernation promoting factor -
  FE240_RS14370 (FE240_14375) rpoN/rpoN1 3025421..3026860 (-) 1440 WP_193001878.1 RNA polymerase factor sigma-54 Machinery gene
  FE240_RS14375 (FE240_14380) lptB 3026914..3027639 (-) 726 WP_193001880.1 LPS export ABC transporter ATP-binding protein -
  FE240_RS14380 (FE240_14385) lptA 3027646..3028167 (-) 522 WP_193004567.1 lipopolysaccharide transport periplasmic protein LptA -
  FE240_RS14385 (FE240_14390) lptC 3028154..3028714 (-) 561 WP_042045428.1 LPS export ABC transporter periplasmic protein LptC -
  FE240_RS14390 (FE240_14395) kdsC 3028711..3029265 (-) 555 WP_193001882.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FE240_RS14395 (FE240_14400) - 3029265..3030260 (-) 996 WP_404800030.1 KpsF/GutQ family sugar-phosphate isomerase -
  FE240_RS14400 (FE240_14405) mlaF 3030470..3031270 (+) 801 WP_193001884.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53797.78 Da        Isoelectric Point: 4.4034

>NTDB_id=364565 FE240_RS14370 WP_193001878.1 3025421..3026860(-) (rpoN/rpoN1) [Aeromonas simiae strain A6]
MKPTLQLRLGQQLTMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQEEVEPDVTSLDSASDNSQMDSSEAMSQEQMP
DELPVDTTWDEIYSAGTAQLAAPSFDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAVDESGYLTVSLED
IHTAVSSDEYDVEMDEVEAVLKRVQRFDPVGVAARSVQECLLIQLGQFAPDLPWLKEAIEVVTHHMDLLGARDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNSVVQGDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARSARNSSDTQF
IRSHLQEARWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKYFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKITDLLAEQGIMVARRTIAKYRESLMIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=364565 FE240_RS14370 WP_193001878.1 3025421..3026860(-) (rpoN/rpoN1) [Aeromonas simiae strain A6]
ATGAAGCCGACACTGCAGTTGCGTCTGGGTCAGCAGTTGACCATGACGCCACAACTACAACAAGCAATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTTCAGCAGGAAATCCAGCAAGCCCTCGAGAGTAACCCCTTGCTGGAGCAGGAAGAAGTCGAAC
CTGATGTCACCTCCCTCGACAGTGCCTCTGATAACAGCCAGATGGACTCTTCAGAGGCCATGTCACAGGAACAAATGCCC
GATGAGTTGCCGGTCGATACGACGTGGGATGAGATCTACTCGGCCGGTACGGCGCAGCTCGCTGCCCCCAGTTTCGATGG
TGAAGATTCTGTCTACCAAGGGGAAACGACCGAAAACTTGCAGGATTATCTGCTGTGGCAGATGCGATTAACCCCGTTTT
CTGATGTGGATGCCGCCATTGCGCTGGCAATTATCGACGCAGTGGATGAGTCCGGCTACCTGACCGTCTCCCTTGAGGAT
ATTCATACGGCAGTCAGCTCGGATGAGTACGATGTGGAGATGGACGAGGTGGAAGCTGTGCTCAAGCGAGTACAGCGTTT
TGACCCGGTTGGGGTGGCCGCCCGTTCTGTGCAGGAGTGCCTGTTGATCCAACTGGGGCAGTTTGCACCAGACCTGCCCT
GGCTCAAGGAGGCCATTGAGGTGGTCACCCATCATATGGATCTGCTAGGTGCCAGGGACTACCGGACGCTAGCCCGCAAG
ACTCGGCTTAAAGAAAGCGATCTCAAGGAGGTGCTGGATCTGATCCAGCAGCTGGAGCCTCGCCCTGGTAACAGCGTGGT
GCAGGGGGATTCCCAGTACGTGATCCCAGATGTGTCCGTGGTGAAGAAGGGGAGCAAATGGGTGGTCGAGCTTAATCCGG
ATAGCGCCCCGCGCATCCGGGTCAACGAGACCTATGCGGCCATGGCACGAAGTGCCCGCAACAGCTCAGATACCCAATTC
ATCCGCTCTCACCTGCAAGAAGCTCGCTGGTTTATCAAGAGCCTGGAGAGCCGCAACGATACTCTGCTCAAAGTGGCGAG
TTGTATCGTAGAACAGCAACAAGGTTTTTTTGAGTACGGTGAAGAGGCGATGAAGCCCATGGTACTCAACGATATTGCCG
AGGCGGTCGAGATGCATGAGTCGACCATCTCACGGGTGACTACCCAAAAATATATGCATACCCCACGCGGTATTTTCGAG
CTCAAATATTTCTTCTCGAGCCACGTCAACACCGAAGGGGGAGGAGAGTGTTCATCGACCGCCATCAGGGCTTTGATCAA
GAAGCTGGTGACGGCCGAAAACCCTGCTAAGCCGCTCAGTGACAGCAAGATCACCGATCTGTTAGCGGAGCAGGGGATCA
TGGTGGCAAGACGGACCATCGCCAAATATCGCGAGTCCCTCATGATCCCACCATCTAATCAGCGCAAGCGCCTGGTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6WZS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.455

100

0.48

Multiple sequence alignment