Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FEM41_RS02865 Genome accession   NZ_CP040428
Coordinates   621992..623425 (+) Length   477 a.a.
NCBI ID   WP_138094260.1    Uniprot ID   A0A4P8YDT0
Organism   Jejubacter calystegiae strain KSNA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 616992..628425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEM41_RS02835 (FEM41_02835) - 617527..618504 (+) 978 WP_138094248.1 calcium/sodium antiporter -
  FEM41_RS02840 (FEM41_02840) kdsD 618517..619503 (+) 987 WP_138094250.1 arabinose-5-phosphate isomerase KdsD -
  FEM41_RS02845 (FEM41_02845) kdsC 619520..620086 (+) 567 WP_138094252.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FEM41_RS02850 (FEM41_02850) lptC 620083..620667 (+) 585 WP_138094254.1 LPS export ABC transporter periplasmic protein LptC -
  FEM41_RS02855 (FEM41_02855) lptA 620636..621178 (+) 543 WP_138094256.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  FEM41_RS02860 (FEM41_02860) lptB 621185..621910 (+) 726 WP_138094258.1 LPS export ABC transporter ATP-binding protein -
  FEM41_RS02865 (FEM41_02865) rpoN/rpoN1 621992..623425 (+) 1434 WP_138094260.1 RNA polymerase factor sigma-54 Machinery gene
  FEM41_RS02870 (FEM41_02870) hpf 623448..623735 (+) 288 WP_138094262.1 ribosome hibernation promoting factor -
  FEM41_RS02875 (FEM41_02875) ptsN 623811..624296 (+) 486 WP_138094265.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FEM41_RS02880 (FEM41_02880) rapZ 624363..625217 (+) 855 WP_138094267.1 RNase adapter RapZ -
  FEM41_RS02885 (FEM41_02885) npr 625214..625486 (+) 273 WP_138094269.1 PTS phosphocarrier protein NPr -
  FEM41_RS02890 (FEM41_02890) yrbL 625703..626347 (+) 645 WP_138094271.1 PhoP regulatory network protein YrbL -
  FEM41_RS02895 (FEM41_02895) mtgA 626362..627087 (-) 726 WP_138094273.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  FEM41_RS02900 (FEM41_02900) elbB 627078..627731 (-) 654 WP_138094275.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53380.03 Da        Isoelectric Point: 4.3408

>NTDB_id=364315 FEM41_RS02865 WP_138094260.1 621992..623425(+) (rpoN/rpoN1) [Jejubacter calystegiae strain KSNA2]
MKQGLQLKLSQQLAMTPQLQQAIRLLQLSTLELQQELNQALESNPLLEQSDPLDEVATTESSDSETLDSRDALEQDSMPE
ELPLDASWDEIYTAGTPSGTGTDYVDDELPIYQGETTQSLQDYLMWQVELTPFSDTDIAIATSIVDAVDDTGYLTITVQD
ILDGMGDEEVTQEEVEAVLKRIQRFDPVGVAARDLRECLLIQLSQFAPETPRLNEAHQIVNDHLDLLANHDFRSLMRVTR
LKEEVLKESVGLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHQGRWVVELNGDSIPRLKINQQYAAMGTSARNDSDSQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEHGEEHMKPMVLADIAQAVDMHESTISRVTTQKYLQSPGGIFELK
YFFSSHVSTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTAMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=364315 FEM41_RS02865 WP_138094260.1 621992..623425(+) (rpoN/rpoN1) [Jejubacter calystegiae strain KSNA2]
ATGAAGCAAGGTTTGCAATTAAAGCTGAGCCAACAGCTTGCCATGACGCCCCAGCTGCAACAGGCCATCCGCCTGCTGCA
GCTCTCCACGCTGGAACTCCAGCAGGAGCTTAACCAGGCGCTGGAAAGCAATCCCCTGCTTGAACAGAGCGACCCCCTTG
ACGAAGTCGCGACCACCGAAAGCAGCGACAGCGAAACGCTCGATAGCCGGGATGCGCTGGAACAAGACTCCATGCCCGAA
GAGCTGCCGCTTGATGCCAGCTGGGACGAGATCTATACCGCAGGCACTCCCTCCGGTACCGGCACTGACTATGTCGACGA
CGAACTGCCCATCTACCAGGGCGAAACCACCCAGTCCCTTCAGGACTACCTGATGTGGCAGGTTGAGCTAACTCCTTTCT
CCGACACCGATATCGCCATCGCGACCTCTATTGTCGACGCCGTCGACGACACCGGCTACCTCACCATCACGGTCCAGGAC
ATTCTGGACGGCATGGGCGATGAAGAGGTGACCCAGGAAGAGGTAGAGGCGGTACTCAAGCGCATTCAGCGCTTCGATCC
GGTTGGCGTGGCTGCCCGCGATTTGCGGGAATGTCTGTTGATCCAGCTTTCTCAGTTCGCGCCCGAAACCCCGCGCCTGA
ATGAGGCGCATCAGATCGTCAACGATCATCTCGATCTGCTGGCCAATCATGATTTTCGCTCGCTGATGCGCGTTACGCGC
CTTAAAGAGGAGGTGCTGAAAGAATCCGTCGGGCTGATTCAGTCCCTCGATCCACGCCCAGGCCAGTCGATTCAGACCAG
CGAGCCGGAATACGTTATCCCTGACGTGCTGGTCCGTAAGCACCAGGGACGCTGGGTTGTGGAACTTAACGGCGACAGTA
TTCCGCGCCTGAAGATTAATCAGCAGTATGCGGCCATGGGGACCAGCGCCCGTAACGACAGCGACAGCCAGTTTATCCGC
AGTAATCTGCAGGAAGCGAAGTGGCTGATTAAGAGTCTGGAGAGCCGTAACGACACCCTGCTACGCGTCAGCCGCTGTAT
TGTGGAACAGCAGCAGGCCTTTTTCGAACATGGTGAAGAGCATATGAAACCCATGGTGCTGGCCGATATCGCCCAGGCCG
TTGATATGCACGAATCCACCATCTCACGCGTCACCACTCAAAAATACCTCCAGAGTCCAGGAGGTATTTTTGAGCTCAAG
TATTTCTTCTCCAGCCACGTGAGCACCGAAGGCGGCGGCGAAGCCTCCTCTACGGCTATCCGGGCGTTGGTGAAGAAATT
AATCGCTGCGGAGAATCCCGCCAAGCCACTGAGCGACAGTAAGCTCACCGCCATGCTCTCCGACCAGGGGATTATGGTGG
CGCGCCGAACCGTTGCGAAGTACCGAGAGTCTTTATCGATTCCTCCGTCTAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P8YDT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment