Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FE003_RS16885 Genome accession   NZ_CP040425
Coordinates   3450977..3451777 (-) Length   266 a.a.
NCBI ID   WP_213076084.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain PB364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3445977..3456777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE003_RS16865 (FE003_16865) - 3447042..3447740 (-) 699 WP_000058788.1 YfgM family protein -
  FE003_RS16870 (FE003_16870) hisS 3447769..3449061 (-) 1293 WP_000095264.1 histidine--tRNA ligase -
  FE003_RS16875 (FE003_16875) ispG 3449058..3450173 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FE003_RS16880 (FE003_16880) - 3450195..3450986 (-) 792 WP_000405164.1 helix-turn-helix domain-containing protein -
  FE003_RS16885 (FE003_16885) pilF 3450977..3451777 (-) 801 WP_213076084.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FE003_RS16890 (FE003_16890) rlmN 3451793..3453025 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FE003_RS16895 (FE003_16895) ndk 3453148..3453579 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  FE003_RS16900 (FE003_16900) iscX 3453693..3453893 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  FE003_RS16905 (FE003_16905) - 3453993..3454307 (-) 315 Protein_3275 phosphatase PAP2 family protein -
  FE003_RS16910 (FE003_16910) - 3454380..3455470 (+) 1091 WP_085940413.1 IS4-like element ISAba1 family transposase -
  FE003_RS16915 (FE003_16915) - 3455491..3455733 (-) 243 Protein_3277 phosphatidylglycerophosphatase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30193.58 Da        Isoelectric Point: 9.3926

>NTDB_id=364297 FE003_RS16885 WP_213076084.1 3450977..3451777(-) (pilF) [Acinetobacter baumannii strain PB364]
MSTPKLKIMLCMGVAVALLTRGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=364297 FE003_RS16885 WP_213076084.1 3450977..3451777(-) (pilF) [Acinetobacter baumannii strain PB364]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGGGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722

Multiple sequence alignment