Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FE321_RS00440 Genome accession   NZ_CP040424
Coordinates   84599..85975 (+) Length   458 a.a.
NCBI ID   WP_208955199.1    Uniprot ID   -
Organism   Dolosigranulum pigrum strain KPL3033     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 79599..90975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE321_RS00420 (FE321_00415) pgeF 81327..82055 (+) 729 WP_112788508.1 peptidoglycan editing factor PgeF -
  FE321_RS00425 (FE321_00420) - 82175..82486 (-) 312 WP_004635215.1 hypothetical protein -
  FE321_RS00430 (FE321_00425) ugpC 82764..83873 (+) 1110 WP_077863087.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  FE321_RS00435 (FE321_00430) - 84010..84546 (+) 537 WP_112767745.1 dUTP diphosphatase -
  FE321_RS00440 (FE321_00435) radA 84599..85975 (+) 1377 WP_208955199.1 DNA repair protein RadA Machinery gene
  FE321_RS00445 (FE321_00440) - 86022..87125 (+) 1104 WP_077863089.1 PIN/TRAM domain-containing protein -
  FE321_RS00450 (FE321_00445) - 87197..87571 (-) 375 WP_111951149.1 hypothetical protein -
  FE321_RS00455 (FE321_00450) - 87617..88369 (-) 753 WP_208955200.1 LytTR family DNA-binding domain-containing protein -
  FE321_RS00460 (FE321_00455) - 89082..90200 (+) 1119 WP_208954300.1 hypothetical protein -
  FE321_RS00465 (FE321_00460) - 90224..90817 (+) 594 WP_004635196.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50102.27 Da        Isoelectric Point: 5.8921

>NTDB_id=364246 FE321_RS00440 WP_208955199.1 84599..85975(+) (radA) [Dolosigranulum pigrum strain KPL3033]
MAKKKVNFMCNECGIESPKYLGRCPSCGAWDSFVEAPKAADEDQKDNRKRVSLTGERATPTLITEVEVEREDRVTVPFSE
FNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQISAFLNNQGAKVLYVSGEESASQIKMRAERLEVVGANFYLYAETDLSYI
QDAIQKLRPDFVIVDSIQTMHHPNIDSASGSVSQVRESTATLMQLAKTNDIGIFIVGHVTKEGSIAGPRMLEHMVDTVLY
FEGEQHHTFRVLRAVKNRFGSTNEIGIFEMNEDGLKEVHNPSQLFLEERLAGTNGSAVVAAIEGTRPILVEIQSLIAPTA
FGHAKRTASGLDYSRVSLIMAVLEKRAGLLLQNYDAYLKAAGGVKLDEPAIDLSIAMSIVSSYKEKETQATDCFIGEIGL
TGEIRRVSKIEARVTEAAKLGFKRVFIPKNNIDGWTHPDGIEVIGCTTVSEVMKRTFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=364246 FE321_RS00440 WP_208955199.1 84599..85975(+) (radA) [Dolosigranulum pigrum strain KPL3033]
ATGGCGAAGAAAAAAGTAAATTTCATGTGCAACGAGTGTGGGATTGAATCTCCGAAGTATTTGGGAAGATGTCCATCTTG
CGGGGCGTGGGATTCCTTCGTAGAAGCGCCAAAAGCAGCGGACGAAGACCAAAAAGATAACCGTAAAAGGGTCTCCTTGA
CGGGGGAGAGAGCGACACCAACCTTAATTACAGAGGTAGAAGTAGAGCGTGAAGATCGTGTGACAGTCCCTTTCAGTGAG
TTCAACCGTGTGCTGGGTGGCGGGATTGTCCCGGGCTCACTCGTCTTGATTGGAGGAGATCCCGGCATTGGGAAGTCCAC
CCTGTTGCTTCAGATCTCAGCTTTCTTGAATAATCAAGGGGCGAAGGTGCTCTACGTTTCGGGGGAAGAGAGTGCTAGCC
AAATTAAGATGCGAGCGGAACGCTTAGAAGTTGTCGGGGCGAACTTTTACTTATATGCGGAGACCGATTTGAGTTATATT
CAAGATGCGATTCAGAAGTTGCGACCGGACTTTGTTATTGTGGATTCGATTCAGACGATGCACCATCCGAATATTGATAG
TGCATCTGGTAGTGTATCGCAAGTGCGTGAATCGACCGCTACCTTGATGCAGCTCGCGAAGACGAATGATATCGGGATCT
TCATTGTCGGTCACGTAACGAAGGAAGGCTCGATTGCGGGGCCACGTATGTTAGAGCATATGGTGGATACCGTGCTTTAC
TTCGAAGGGGAACAACATCATACGTTCCGAGTCTTGCGCGCCGTGAAGAATCGGTTCGGTTCGACTAACGAAATTGGTAT
CTTCGAGATGAATGAAGATGGACTAAAAGAAGTGCATAATCCGAGTCAGCTATTCTTAGAAGAGCGGCTGGCGGGGACGA
ATGGCTCAGCTGTTGTCGCTGCAATAGAGGGAACGCGTCCGATTTTAGTAGAGATTCAGTCGCTCATTGCCCCGACTGCC
TTTGGTCATGCCAAGCGAACAGCAAGCGGCCTGGATTACTCGCGCGTCTCCTTAATCATGGCTGTATTGGAGAAGCGAGC
AGGCCTTTTATTACAAAATTATGATGCCTACTTGAAGGCAGCAGGTGGCGTGAAGTTAGATGAGCCGGCTATTGACTTAA
GTATCGCGATGAGCATCGTCTCCAGCTATAAGGAAAAAGAAACCCAAGCCACGGATTGCTTTATCGGAGAGATTGGCTTA
ACTGGCGAAATTCGACGAGTCTCTAAAATTGAAGCACGGGTTACGGAAGCAGCTAAGTTAGGCTTCAAGCGTGTCTTTAT
TCCGAAGAATAATATAGACGGCTGGACGCATCCTGACGGTATCGAAGTCATCGGCTGTACCACTGTCTCAGAAGTAATGA
AGCGAACATTTAACTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.646

99.782

0.655
  radA Streptococcus pneumoniae Rx1

65.646

99.782

0.655
  radA Streptococcus pneumoniae D39

65.646

99.782

0.655
  radA Streptococcus pneumoniae R6

65.646

99.782

0.655
  radA Streptococcus pneumoniae TIGR4

65.646

99.782

0.655
  radA Streptococcus mitis SK321

65.646

99.782

0.655
  radA Bacillus subtilis subsp. subtilis str. 168

63.537

100

0.635

Multiple sequence alignment