Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FE322_RS00450 Genome accession   NZ_CP040423
Coordinates   90734..92110 (+) Length   458 a.a.
NCBI ID   WP_111951151.1    Uniprot ID   -
Organism   Dolosigranulum pigrum strain KPL3043     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 85734..97110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE322_RS00430 (FE322_00445) pgeF 87462..88190 (+) 729 WP_201711365.1 peptidoglycan editing factor PgeF -
  FE322_RS00435 (FE322_00450) - 88310..88621 (-) 312 WP_004635215.1 hypothetical protein -
  FE322_RS00440 (FE322_00455) ugpC 88899..90008 (+) 1110 WP_077863087.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  FE322_RS00445 (FE322_00460) - 90145..90681 (+) 537 WP_208953398.1 dUTP diphosphatase -
  FE322_RS00450 (FE322_00465) radA 90734..92110 (+) 1377 WP_111951151.1 DNA repair protein RadA Machinery gene
  FE322_RS00455 (FE322_00470) - 92157..93260 (+) 1104 WP_208969558.1 PIN/TRAM domain-containing protein -
  FE322_RS00460 (FE322_00475) - 93332..93718 (-) 387 WP_004635204.1 hypothetical protein -
  FE322_RS00465 - 94180..94347 (+) 168 WP_172764097.1 hypothetical protein -
  FE322_RS00470 (FE322_00480) - 94432..95550 (+) 1119 WP_208954300.1 hypothetical protein -
  FE322_RS00475 (FE322_00485) - 95574..96167 (+) 594 WP_004635196.1 ATP-binding cassette domain-containing protein -
  FE322_RS00480 (FE322_00490) - 96148..96642 (+) 495 WP_208954301.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50104.30 Da        Isoelectric Point: 5.8932

>NTDB_id=364223 FE322_RS00450 WP_111951151.1 90734..92110(+) (radA) [Dolosigranulum pigrum strain KPL3043]
MAKKKVNFMCNECGIESPKYLGRCPSCGAWDSFVEAPKAADEDQKDNRKRVSLTGERATPTLITEVEVEREDRVTVPFSE
FNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQISAFLNNQGAKVLYVSGEESASQIKMRAERLEVVGANFYLYAETDLSYI
QDAIQKLRPDFVIVDSIQTMHHPNIDSASGSVSQVRESTATLMQLAKTNDIGIFIVGHVTKEGSIAGPRMLEHMVDTVLY
FEGEQHHTFRVLRAVKNRFGSTNEIGIFEMNEDGLKEVHNPSQLFLEERLAGTNGSAVVAAMEGTRPILVEIQSLIAPTA
FGHAKRTASGLDYSRVSLIMAVLEKRAGLLLQNYDAYLKAAGGVKLDEPAIDLSIAMSIVSSYKEKETQATDCFIGEIGL
TGEIRRVSKIEARVTEAAKLGFKRVFIPKNNIDGWTHPEGIEVIGCATVSEVMKRTFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=364223 FE322_RS00450 WP_111951151.1 90734..92110(+) (radA) [Dolosigranulum pigrum strain KPL3043]
ATGGCGAAGAAAAAAGTAAATTTCATGTGCAACGAGTGTGGGATTGAATCTCCGAAGTATTTGGGAAGATGTCCATCTTG
CGGGGCGTGGGATTCCTTCGTAGAAGCGCCAAAAGCAGCGGATGAAGACCAAAAAGATAACCGCAAAAGGGTCTCCTTAA
CAGGGGAGAGAGCGACACCGACCTTAATTACAGAGGTAGAAGTAGAGCGTGAAGATCGTGTGACAGTCCCTTTCAGTGAG
TTCAACCGTGTGCTGGGTGGCGGGATTGTTCCGGGATCACTTGTCTTGATTGGAGGAGATCCCGGCATTGGGAAGTCCAC
CCTGCTGCTTCAGATCTCAGCTTTCTTGAATAATCAAGGGGCGAAGGTGCTCTACGTTTCGGGGGAAGAGAGTGCTAGCC
AAATTAAGATGCGAGCGGAACGCTTAGAAGTTGTCGGGGCGAACTTTTACTTATATGCAGAGACTGATTTGAGTTATATT
CAAGATGCGATTCAGAAGTTGCGACCGGACTTTGTTATTGTGGATTCGATTCAGACGATGCACCATCCGAATATTGATAG
TGCATCTGGTAGTGTATCGCAAGTGCGTGAATCGACCGCTACCTTGATGCAGCTCGCGAAGACGAATGATATCGGGATCT
TCATTGTTGGTCACGTGACGAAGGAAGGCTCGATTGCGGGTCCACGTATGTTAGAGCATATGGTGGATACCGTGCTTTAC
TTCGAAGGGGAACAACATCATACGTTCCGAGTCTTGCGCGCCGTGAAGAATCGGTTCGGTTCGACCAACGAGATTGGTAT
CTTCGAGATGAATGAAGATGGACTAAAAGAAGTGCATAATCCGAGTCAGCTATTCTTAGAAGAGCGGCTGGCGGGGACGA
ATGGCTCAGCCGTTGTTGCTGCAATGGAGGGAACGCGTCCGATTTTGGTAGAGATTCAGTCGCTCATTGCCCCGACTGCC
TTTGGTCATGCCAAGCGAACAGCAAGCGGCCTGGATTATTCGCGCGTCTCCTTAATCATGGCTGTATTAGAGAAGCGAGC
AGGGCTTTTATTACAAAATTATGATGCCTACTTGAAGGCAGCAGGTGGAGTGAAGTTAGATGAACCGGCGATTGACTTAA
GTATCGCGATGAGCATCGTCTCCAGCTATAAGGAAAAAGAAACCCAAGCCACGGATTGCTTTATCGGAGAGATTGGCTTA
ACTGGCGAAATTCGACGAGTCTCTAAAATTGAAGCACGGGTTACGGAAGCGGCTAAGTTAGGCTTCAAGCGTGTCTTTAT
TCCGAAGAATAATATAGACGGCTGGACGCATCCTGAGGGTATCGAAGTCATCGGCTGTGCCACTGTCTCAGAAGTAATGA
AGCGAACATTTAACTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.646

99.782

0.655
  radA Streptococcus pneumoniae Rx1

65.646

99.782

0.655
  radA Streptococcus pneumoniae D39

65.646

99.782

0.655
  radA Streptococcus pneumoniae R6

65.646

99.782

0.655
  radA Streptococcus pneumoniae TIGR4

65.646

99.782

0.655
  radA Streptococcus mitis SK321

65.646

99.782

0.655
  radA Bacillus subtilis subsp. subtilis str. 168

63.974

100

0.64

Multiple sequence alignment