Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FE323_RS00435 Genome accession   NZ_CP040422
Coordinates   84514..85890 (+) Length   458 a.a.
NCBI ID   WP_111974160.1    Uniprot ID   A0A328K5R9
Organism   Dolosigranulum pigrum strain KPL3050     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 79514..90890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE323_RS00415 (FE323_00420) pgeF 81242..81970 (+) 729 WP_004635217.1 peptidoglycan editing factor PgeF -
  FE323_RS00420 (FE323_00425) - 82090..82401 (-) 312 WP_208955981.1 iron-sulfur cluster biosynthesis protein -
  FE323_RS00425 (FE323_00430) ugpC 82679..83788 (+) 1110 WP_077863087.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  FE323_RS00430 (FE323_00435) - 83925..84461 (+) 537 WP_208955982.1 dUTP diphosphatase -
  FE323_RS00435 (FE323_00440) radA 84514..85890 (+) 1377 WP_111974160.1 DNA repair protein RadA Machinery gene
  FE323_RS00440 (FE323_00445) - 85937..87040 (+) 1104 WP_004635206.1 PIN/TRAM domain-containing protein -
  FE323_RS00445 (FE323_00450) - 87111..87485 (-) 375 WP_208959453.1 hypothetical protein -
  FE323_RS00450 (FE323_00455) - 87531..88283 (-) 753 WP_201711362.1 LytTR family DNA-binding domain-containing protein -
  FE323_RS00455 (FE323_00460) - 88735..88911 (+) 177 WP_208959454.1 hypothetical protein -
  FE323_RS00460 (FE323_00465) - 88996..90114 (+) 1119 WP_004635199.1 hypothetical protein -
  FE323_RS00465 (FE323_00470) - 90138..90731 (+) 594 WP_004635196.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50090.27 Da        Isoelectric Point: 5.8921

>NTDB_id=364201 FE323_RS00435 WP_111974160.1 84514..85890(+) (radA) [Dolosigranulum pigrum strain KPL3050]
MAKKKVNFMCNECGIESPKYLGRCPSCGAWDSFVEAPKAADEDQKDNRKRVSLTGERATPTLITEVEVEREDRVTVPFSE
FNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQISAFLNNQGAKVLYVSGEESASQIKMRAERLEVVGANFYLYAETDLSYI
QDAIQKLRPDFVIVDSIQTMHHPNIDSASGSVSQVRESTATLMQLAKTNDIGIFIVGHVTKEGSIAGPRMLEHMVDTVLY
FEGEQHHTFRVLRAVKNRFGSTNEIGIFEMNEDGLKEVHNPSQLFLEERLAGTNGSAVVAAMEGTRPILVEIQSLIAPTA
FGHAKRTASGLDYSRVSLIMAVLEKRAGLLLQNYDAYLKAAGGVKLDEPAIDLSIAMSIVSSYKEKETQATDCFIGEIGL
TGEIRRVSKIEARVTEAAKLGFKRVFIPKNNIDGWTHPDGIEVIGCATVSEVMKRTFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=364201 FE323_RS00435 WP_111974160.1 84514..85890(+) (radA) [Dolosigranulum pigrum strain KPL3050]
ATGGCGAAGAAAAAAGTAAATTTCATGTGCAACGAGTGTGGGATTGAATCTCCGAAGTATTTGGGAAGATGTCCATCTTG
CGGGGCGTGGGATTCCTTCGTAGAAGCGCCAAAAGCAGCGGACGAAGACCAAAAAGATAACCGCAAAAGGGTCTCCTTAA
CAGGGGAGAGAGCGACACCGACCTTAATTACAGAGGTAGAAGTAGAGCGTGAAGATCGTGTGACAGTCCCTTTCAGTGAG
TTCAACCGTGTGCTGGGAGGCGGGATTGTCCCGGGCTCACTTGTCTTGATCGGAGGAGATCCCGGCATTGGGAAGTCCAC
CCTGTTGCTTCAGATCTCAGCTTTCTTGAATAATCAAGGGGCGAAGGTGCTCTACGTTTCGGGGGAAGAGAGTGCTAGCC
AAATTAAGATGCGAGCGGAACGCTTAGAAGTTGTCGGGGCGAACTTTTACTTATATGCGGAGACTGATTTGAGTTATATT
CAAGATGCGATTCAGAAGTTGCGACCGGACTTTGTTATTGTGGATTCGATTCAGACGATGCACCATCCGAATATTGATAG
TGCATCTGGGAGTGTATCGCAAGTGCGTGAATCGACCGCTACCTTGATGCAGCTCGCGAAGACGAATGATATCGGGATCT
TCATTGTTGGTCACGTGACGAAGGAAGGCTCGATTGCGGGTCCACGTATGTTAGAGCATATGGTGGACACTGTGCTTTAC
TTCGAAGGGGAACAACATCATACGTTCCGAGTCTTGCGCGCCGTGAAAAATCGGTTCGGTTCGACTAACGAGATTGGTAT
CTTCGAGATGAATGAAGATGGACTAAAAGAAGTGCATAATCCGAGTCAGCTGTTCTTAGAAGAGCGGCTGGCGGGGACGA
ATGGCTCTGCTGTTGTCGCTGCAATGGAGGGAACGCGTCCGATTTTAGTAGAGATTCAGTCGCTCATTGCCCCGACTGCC
TTTGGTCATGCCAAGCGAACAGCAAGTGGCCTGGATTACTCGCGCGTCTCCTTAATCATGGCTGTATTGGAGAAGCGAGC
AGGCCTTTTATTACAAAATTATGATGCCTACTTGAAGGCAGCAGGTGGCGTGAAGTTAGATGAACCGGCGATTGACTTAA
GTATCGCGATGAGCATCGTCTCCAGCTATAAGGAAAAAGAAACCCAAGCCACGGATTGCTTTATCGGAGAGATTGGCTTA
ACTGGCGAAATTCGACGAGTCTCTAAAATTGAAGCACGGGTTACGGAAGCAGCTAAGTTAGGCTTCAAGCGTGTCTTTAT
TCCAAAGAATAATATAGACGGCTGGACGCATCCTGACGGTATCGAAGTCATCGGCTGTGCCACTGTCTCAGAAGTAATGA
AGCGAACATTTAACTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A328K5R9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.646

99.782

0.655
  radA Streptococcus pneumoniae Rx1

65.646

99.782

0.655
  radA Streptococcus pneumoniae D39

65.646

99.782

0.655
  radA Streptococcus pneumoniae R6

65.646

99.782

0.655
  radA Streptococcus pneumoniae TIGR4

65.646

99.782

0.655
  radA Streptococcus mitis SK321

65.646

99.782

0.655
  radA Bacillus subtilis subsp. subtilis str. 168

63.974

100

0.64

Multiple sequence alignment