Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FE326_RS00430 Genome accession   NZ_CP040419
Coordinates   83942..85318 (+) Length   458 a.a.
NCBI ID   WP_111951151.1    Uniprot ID   -
Organism   Dolosigranulum pigrum strain KPL3069     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 78942..90318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE326_RS00410 (FE326_00415) pgeF 80680..81408 (+) 729 WP_208954297.1 peptidoglycan editing factor PgeF -
  FE326_RS00415 (FE326_00420) - 81526..81837 (-) 312 WP_004635215.1 hypothetical protein -
  FE326_RS00420 (FE326_00425) ugpC 82108..83217 (+) 1110 WP_077863087.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  FE326_RS00425 (FE326_00430) - 83354..83890 (+) 537 WP_111974161.1 dUTP diphosphatase -
  FE326_RS00430 (FE326_00435) radA 83942..85318 (+) 1377 WP_111951151.1 DNA repair protein RadA Machinery gene
  FE326_RS00435 (FE326_00440) - 85365..86468 (+) 1104 WP_077863089.1 PIN/TRAM domain-containing protein -
  FE326_RS00440 (FE326_00445) - 86540..86731 (-) 192 WP_208954298.1 hypothetical protein -
  FE326_RS00445 (FE326_00450) - 86728..86925 (-) 198 WP_208954299.1 hypothetical protein -
  FE326_RS00450 (FE326_00455) - 87638..88756 (+) 1119 WP_208954300.1 hypothetical protein -
  FE326_RS00455 (FE326_00460) - 88780..89373 (+) 594 WP_004635196.1 ATP-binding cassette domain-containing protein -
  FE326_RS00460 (FE326_00465) - 89354..89848 (+) 495 WP_208954301.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50104.30 Da        Isoelectric Point: 5.8932

>NTDB_id=364131 FE326_RS00430 WP_111951151.1 83942..85318(+) (radA) [Dolosigranulum pigrum strain KPL3069]
MAKKKVNFMCNECGIESPKYLGRCPSCGAWDSFVEAPKAADEDQKDNRKRVSLTGERATPTLITEVEVEREDRVTVPFSE
FNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQISAFLNNQGAKVLYVSGEESASQIKMRAERLEVVGANFYLYAETDLSYI
QDAIQKLRPDFVIVDSIQTMHHPNIDSASGSVSQVRESTATLMQLAKTNDIGIFIVGHVTKEGSIAGPRMLEHMVDTVLY
FEGEQHHTFRVLRAVKNRFGSTNEIGIFEMNEDGLKEVHNPSQLFLEERLAGTNGSAVVAAMEGTRPILVEIQSLIAPTA
FGHAKRTASGLDYSRVSLIMAVLEKRAGLLLQNYDAYLKAAGGVKLDEPAIDLSIAMSIVSSYKEKETQATDCFIGEIGL
TGEIRRVSKIEARVTEAAKLGFKRVFIPKNNIDGWTHPEGIEVIGCATVSEVMKRTFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=364131 FE326_RS00430 WP_111951151.1 83942..85318(+) (radA) [Dolosigranulum pigrum strain KPL3069]
ATGGCGAAGAAAAAAGTAAATTTCATGTGCAACGAGTGTGGGATTGAATCTCCGAAGTATTTGGGAAGATGTCCATCTTG
CGGGGCGTGGGATTCCTTCGTAGAAGCGCCAAAAGCAGCGGACGAAGACCAAAAAGATAACCGCAAAAGGGTCTCCTTAA
CAGGGGAGAGAGCGACACCGACCTTAATTACAGAAGTAGAAGTAGAGCGTGAAGATCGTGTGACAGTCCCTTTCAGTGAG
TTCAACCGTGTGCTGGGTGGCGGGATTGTCCCGGGATCACTCGTCTTGATTGGAGGAGATCCCGGCATTGGGAAGTCCAC
CCTGTTGCTTCAGATCTCAGCTTTCTTGAATAATCAAGGGGCGAAGGTGCTCTACGTTTCGGGCGAAGAGAGTGCTAGCC
AAATTAAGATGCGAGCGGAACGCTTAGAAGTTGTTGGGGCGAACTTTTACTTATATGCGGAGACTGATTTGAGTTATATT
CAAGATGCGATTCAGAAGTTGCGACCGGACTTTGTTATTGTGGATTCGATTCAGACGATGCACCATCCGAATATTGATAG
TGCATCCGGTAGTGTATCGCAAGTGCGTGAATCGACCGCCACCTTGATGCAGCTCGCGAAGACGAATGATATCGGGATCT
TCATTGTCGGTCACGTAACGAAGGAAGGCTCGATTGCGGGGCCACGTATGTTAGAGCATATGGTGGATACCGTGCTTTAC
TTCGAAGGGGAACAACATCATACGTTCCGAGTCTTGCGCGCCGTGAAGAATCGGTTCGGTTCGACTAACGAGATTGGTAT
CTTCGAGATGAATGAAGATGGACTAAAAGAAGTGCATAATCCGAGTCAACTATTCTTAGAAGAACGGCTGGCGGGGACGA
ATGGCTCAGCCGTTGTTGCTGCAATGGAGGGAACGCGTCCGATTTTAGTAGAGATTCAGTCGCTCATTGCCCCGACTGCC
TTTGGTCATGCCAAGCGAACAGCAAGTGGCCTGGATTACTCGCGCGTCTCCTTAATCATGGCTGTATTGGAGAAGCGAGC
AGGGCTTTTATTACAAAATTATGATGCCTACTTGAAGGCAGCAGGTGGCGTGAAGTTAGATGAGCCGGCTATTGACTTAA
GTATCGCGATGAGCATCGTCTCCAGCTATAAGGAAAAAGAAACCCAAGCCACGGACTGCTTTATCGGAGAGATTGGCTTA
ACTGGCGAAATTCGACGAGTCTCTAAAATTGAAGCACGGGTTACGGAAGCGGCTAAGTTAGGCTTCAAGCGTGTCTTTAT
TCCGAAGAATAATATAGACGGCTGGACGCATCCTGAGGGTATCGAAGTCATCGGCTGTGCCACTGTCTCAGAAGTAATGA
AGCGAACATTTAACTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.646

99.782

0.655
  radA Streptococcus pneumoniae Rx1

65.646

99.782

0.655
  radA Streptococcus pneumoniae D39

65.646

99.782

0.655
  radA Streptococcus pneumoniae R6

65.646

99.782

0.655
  radA Streptococcus pneumoniae TIGR4

65.646

99.782

0.655
  radA Streptococcus mitis SK321

65.646

99.782

0.655
  radA Bacillus subtilis subsp. subtilis str. 168

63.974

100

0.64

Multiple sequence alignment