Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FE329_RS00450 Genome accession   NZ_CP040416
Coordinates   88245..89621 (+) Length   458 a.a.
NCBI ID   WP_208966495.1    Uniprot ID   -
Organism   Dolosigranulum pigrum strain KPL3084     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 83245..94621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE329_RS00430 (FE329_00435) pgeF 84973..85701 (+) 729 WP_208966492.1 peptidoglycan editing factor PgeF -
  FE329_RS00435 (FE329_00440) - 85821..86132 (-) 312 WP_208966493.1 iron-sulfur cluster biosynthesis protein -
  FE329_RS00440 (FE329_00445) ugpC 86410..87519 (+) 1110 WP_004635212.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  FE329_RS00445 (FE329_00450) - 87656..88192 (+) 537 WP_208966494.1 dUTP diphosphatase -
  FE329_RS00450 (FE329_00455) radA 88245..89621 (+) 1377 WP_208966495.1 DNA repair protein RadA Machinery gene
  FE329_RS00455 (FE329_00460) - 89668..90771 (+) 1104 WP_004635206.1 PIN/TRAM domain-containing protein -
  FE329_RS00460 (FE329_00465) - 90843..91217 (-) 375 WP_208957393.1 hypothetical protein -
  FE329_RS00465 (FE329_00470) - 91263..92015 (-) 753 WP_208953400.1 LytTR family DNA-binding domain-containing protein -
  FE329_RS00470 (FE329_00475) - 92832..93041 (+) 210 WP_208953401.1 hypothetical protein -
  FE329_RS00475 (FE329_00480) - 93692..94405 (+) 714 WP_112779166.1 type II CAAX endopeptidase family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50103.32 Da        Isoelectric Point: 6.0785

>NTDB_id=364085 FE329_RS00450 WP_208966495.1 88245..89621(+) (radA) [Dolosigranulum pigrum strain KPL3084]
MAKKKVNFMCNECGIESPKYLGRCPSCGAWDSFVEAPKAADEDQKDNRKRVSLTGERATPTLITEVEVEREDRVTVPFSE
FNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQISAFLNNQGAKVLYVSGEESASQIKMRAERLEVVGANFYLYAETDLSYI
QDAIQKLRPNFVIVDSIQTMHHPNIDSASGSVSQVRESTATLMQLAKTNDIGIFIVGHVTKEGSIAGPRMLEHMVDTVLY
FEGEQHHTFRVLRAVKNRFGSTNEIGIFEMNEDGLKEVHNPSQLFLEERLAGTNGSAVVAAMEGTRPILVEIQSLIAPTA
FGHAKRTASGLDYSRVSLIMAVLEKRAGLLLQNYDAYLKAAGGVKLDEPAIDLSIAMSIVSSYKEKETQATDCFIGEIGL
TGEIRRVSKIEARVTEAAKLGFKRVFIPKNNIDGWTHPEGIEVIGCATVSEVMKRTFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=364085 FE329_RS00450 WP_208966495.1 88245..89621(+) (radA) [Dolosigranulum pigrum strain KPL3084]
ATGGCGAAGAAAAAAGTAAATTTCATGTGCAACGAGTGTGGGATTGAATCTCCGAAGTATTTGGGAAGATGTCCATCTTG
CGGGGCGTGGGATTCCTTCGTAGAAGCGCCAAAAGCAGCGGACGAAGACCAAAAAGATAACCGCAAAAGGGTCTCCTTAA
CAGGGGAGAGAGCGACACCGACCTTAATTACAGAGGTAGAAGTAGAGCGTGAAGATCGTGTGACAGTCCCTTTCAGTGAG
TTCAACCGTGTGCTGGGAGGCGGGATTGTCCCAGGCTCACTCGTCTTGATCGGAGGAGATCCCGGCATTGGGAAGTCCAC
CCTGTTGCTTCAGATCTCAGCTTTCTTGAATAATCAAGGGGCGAAGGTGCTCTACGTTTCGGGGGAAGAGAGTGCTAGCC
AAATTAAGATGCGAGCGGAACGCTTAGAAGTTGTCGGGGCGAACTTTTACTTATATGCGGAGACTGATTTGAGTTATATT
CAAGATGCGATTCAGAAGTTGCGACCGAACTTTGTTATTGTGGATTCGATTCAGACGATGCACCATCCGAATATTGATAG
TGCATCTGGTAGTGTATCGCAAGTGCGTGAATCGACCGCTACCTTGATGCAGCTCGCGAAGACGAATGATATTGGGATCT
TCATTGTTGGTCACGTGACGAAGGAAGGCTCGATTGCGGGGCCACGTATGTTAGAGCATATGGTGGACACTGTGCTTTAC
TTCGAAGGGGAACAACATCATACGTTCCGAGTCTTGCGCGCCGTGAAAAATCGGTTCGGTTCGACTAACGAGATTGGTAT
CTTCGAGATGAATGAAGATGGACTAAAAGAAGTGCATAATCCGAGTCAGCTATTCTTAGAAGAGCGGCTGGCGGGGACGA
ATGGCTCAGCCGTTGTTGCTGCAATGGAGGGAACGCGTCCGATTTTAGTAGAGATTCAGTCGCTCATTGCCCCGACTGCC
TTTGGTCATGCCAAGCGAACTGCAAGCGGCCTGGATTATTCGCGCGTCTCCTTAATCATGGCTGTATTGGAGAAGCGAGC
AGGCCTTTTATTACAAAATTATGATGCCTACTTGAAGGCAGCAGGTGGAGTGAAGTTAGATGAGCCGGCGATTGACTTAA
GTATCGCGATGAGCATCGTCTCCAGCTATAAGGAAAAAGAAACCCAAGCCACGGATTGCTTTATCGGAGAGATTGGCTTA
ACTGGCGAAATTCGACGAGTCTCTAAAATTGAAGCACGGGTTACGGAAGCAGCTAAGTTAGGCTTCAAGCGTGTCTTTAT
TCCCAAGAATAATATAGACGGCTGGACGCATCCTGAGGGTATCGAAGTCATCGGCTGTGCCACTGTCTCAGAAGTAATGA
AGCGAACATTTAACTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.427

99.782

0.653
  radA Streptococcus pneumoniae Rx1

65.427

99.782

0.653
  radA Streptococcus pneumoniae D39

65.427

99.782

0.653
  radA Streptococcus pneumoniae R6

65.427

99.782

0.653
  radA Streptococcus pneumoniae TIGR4

65.427

99.782

0.653
  radA Streptococcus mitis SK321

65.427

99.782

0.653
  radA Bacillus subtilis subsp. subtilis str. 168

63.974

100

0.64

Multiple sequence alignment