Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FE333_RS00415 Genome accession   NZ_CP040412
Coordinates   83050..84426 (+) Length   458 a.a.
NCBI ID   WP_111951151.1    Uniprot ID   -
Organism   Dolosigranulum pigrum strain KPL3250     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 78050..89426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE333_RS00395 (FE333_00400) pgeF 79781..80509 (+) 729 WP_142970545.1 peptidoglycan editing factor PgeF -
  FE333_RS00400 (FE333_00405) - 80627..80938 (-) 312 WP_004635215.1 hypothetical protein -
  FE333_RS00405 (FE333_00410) ugpC 81216..82325 (+) 1110 WP_077863087.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  FE333_RS00410 (FE333_00415) - 82462..82998 (+) 537 WP_142970544.1 dUTP diphosphatase -
  FE333_RS00415 (FE333_00420) radA 83050..84426 (+) 1377 WP_111951151.1 DNA repair protein RadA Machinery gene
  FE333_RS00420 (FE333_00425) - 84473..85576 (+) 1104 WP_004635206.1 PIN/TRAM domain-containing protein -
  FE333_RS00425 (FE333_00430) - 85648..86022 (-) 375 WP_208957393.1 hypothetical protein -
  FE333_RS00430 (FE333_00435) - 86068..86820 (-) 753 WP_208953400.1 LytTR family DNA-binding domain-containing protein -
  FE333_RS00435 (FE333_00440) - 87637..87846 (+) 210 WP_208953401.1 hypothetical protein -
  FE333_RS00440 (FE333_00445) - 88497..89210 (+) 714 WP_112779166.1 type II CAAX endopeptidase family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50104.30 Da        Isoelectric Point: 5.8932

>NTDB_id=363994 FE333_RS00415 WP_111951151.1 83050..84426(+) (radA) [Dolosigranulum pigrum strain KPL3250]
MAKKKVNFMCNECGIESPKYLGRCPSCGAWDSFVEAPKAADEDQKDNRKRVSLTGERATPTLITEVEVEREDRVTVPFSE
FNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQISAFLNNQGAKVLYVSGEESASQIKMRAERLEVVGANFYLYAETDLSYI
QDAIQKLRPDFVIVDSIQTMHHPNIDSASGSVSQVRESTATLMQLAKTNDIGIFIVGHVTKEGSIAGPRMLEHMVDTVLY
FEGEQHHTFRVLRAVKNRFGSTNEIGIFEMNEDGLKEVHNPSQLFLEERLAGTNGSAVVAAMEGTRPILVEIQSLIAPTA
FGHAKRTASGLDYSRVSLIMAVLEKRAGLLLQNYDAYLKAAGGVKLDEPAIDLSIAMSIVSSYKEKETQATDCFIGEIGL
TGEIRRVSKIEARVTEAAKLGFKRVFIPKNNIDGWTHPEGIEVIGCATVSEVMKRTFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=363994 FE333_RS00415 WP_111951151.1 83050..84426(+) (radA) [Dolosigranulum pigrum strain KPL3250]
ATGGCGAAGAAAAAAGTAAATTTCATGTGCAACGAGTGTGGGATTGAATCTCCGAAGTATTTGGGAAGATGCCCATCTTG
CGGGGCGTGGGATTCCTTCGTAGAAGCGCCAAAAGCAGCGGACGAAGACCAAAAAGATAACCGTAAAAGGGTCTCCTTAA
CAGGGGAGAGAGCGACACCGACCTTAATTACAGAGGTAGAAGTAGAGCGTGAAGATCGTGTGACAGTCCCTTTCAGTGAG
TTCAACCGTGTGCTGGGTGGCGGGATTGTCCCGGGATCACTCGTCTTGATCGGAGGAGATCCCGGCATTGGGAAGTCCAC
CCTGTTGCTTCAGATCTCAGCTTTCTTGAATAATCAAGGGGCGAAGGTGCTCTACGTTTCGGGGGAAGAGAGTGCTAGCC
AAATTAAGATGCGAGCGGAACGCTTAGAAGTTGTCGGGGCGAACTTTTACTTATATGCAGAGACTGATTTGAGTTATATT
CAAGATGCGATTCAGAAGTTGCGACCGGACTTTGTTATTGTGGATTCGATTCAGACGATGCACCATCCGAATATTGATAG
TGCATCTGGTAGTGTATCGCAAGTGCGTGAATCGACCGCTACCTTGATGCAGCTCGCGAAGACGAATGATATTGGGATCT
TCATTGTTGGTCACGTGACGAAGGAAGGCTCGATTGCGGGGCCACGTATGTTAGAGCATATGGTGGATACCGTGCTTTAC
TTCGAAGGGGAACAACATCATACGTTCCGAGTCTTGCGCGCCGTGAAGAATCGGTTTGGTTCGACTAACGAAATTGGTAT
CTTCGAGATGAATGAAGATGGACTAAAAGAAGTGCATAATCCGAGTCAGCTATTCTTAGAAGAGCGGCTGGCGGGGACGA
ATGGCTCAGCCGTTGTCGCTGCAATGGAGGGAACGCGTCCGATTTTAGTAGAGATTCAGTCGCTCATTGCCCCGACTGCC
TTTGGTCATGCCAAGCGAACTGCAAGCGGCCTGGATTATTCGCGCGTCTCCTTAATCATGGCTGTATTGGAGAAGCGAGC
AGGCCTTTTATTACAAAATTATGATGCCTACTTGAAGGCAGCAGGTGGAGTGAAGTTAGATGAGCCGGCGATTGACTTAA
GTATCGCGATGAGCATCGTCTCCAGCTATAAGGAAAAAGAAACCCAAGCCACGGATTGCTTTATCGGAGAGATTGGCTTA
ACTGGCGAAATTCGACGAGTCTCTAAAATTGAAGCACGGGTTACGGAAGCAGCTAAGTTAGGCTTCAAGCGTGTCTTTAT
TCCCAAGAATAATATAGACGGCTGGACGCATCCTGAGGGTATCGAAGTCATCGGCTGTGCCACTGTCTCAGAAGTAATGA
AGCGAACATTTAACTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.646

99.782

0.655
  radA Streptococcus pneumoniae Rx1

65.646

99.782

0.655
  radA Streptococcus pneumoniae D39

65.646

99.782

0.655
  radA Streptococcus pneumoniae R6

65.646

99.782

0.655
  radA Streptococcus pneumoniae TIGR4

65.646

99.782

0.655
  radA Streptococcus mitis SK321

65.646

99.782

0.655
  radA Bacillus subtilis subsp. subtilis str. 168

63.974

100

0.64

Multiple sequence alignment