Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FEI17_RS02540 Genome accession   NZ_CP040392
Coordinates   529872..531305 (-) Length   477 a.a.
NCBI ID   WP_035887975.1    Uniprot ID   -
Organism   Kosakonia radicincitans strain DSM 107547     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524872..536305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEI17_RS02510 (FEI17_02510) elbB 526440..527093 (+) 654 WP_079517294.1 isoprenoid biosynthesis glyoxalase ElbB -
  FEI17_RS02515 (FEI17_02515) mtgA 527090..527821 (+) 732 WP_079517295.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  FEI17_RS02520 (FEI17_02520) npr 527823..528095 (-) 273 WP_007369893.1 PTS phosphocarrier protein NPr -
  FEI17_RS02525 (FEI17_02525) rapZ 528092..528946 (-) 855 WP_007369894.1 RNase adapter RapZ -
  FEI17_RS02530 (FEI17_02530) ptsN 528989..529480 (-) 492 WP_007369895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FEI17_RS02535 (FEI17_02535) hpf 529562..529849 (-) 288 WP_007369896.1 ribosome hibernation promoting factor -
  FEI17_RS02540 (FEI17_02540) rpoN/rpoN1 529872..531305 (-) 1434 WP_035887975.1 RNA polymerase factor sigma-54 Machinery gene
  FEI17_RS02545 (FEI17_02545) lptB 531354..532079 (-) 726 WP_007369898.1 LPS export ABC transporter ATP-binding protein -
  FEI17_RS02550 (FEI17_02550) lptA 532086..532640 (-) 555 WP_007369899.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  FEI17_RS02555 (FEI17_02555) lptC 532609..533184 (-) 576 WP_071921601.1 LPS export ABC transporter periplasmic protein LptC -
  FEI17_RS02560 (FEI17_02560) kdsC 533181..533747 (-) 567 WP_035887972.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FEI17_RS02565 (FEI17_02565) kdsD 533762..534748 (-) 987 WP_007369902.1 arabinose-5-phosphate isomerase KdsD -
  FEI17_RS02570 (FEI17_02570) - 534762..535736 (-) 975 WP_007369903.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53802.57 Da        Isoelectric Point: 4.3889

>NTDB_id=363878 FEI17_RS02540 WP_035887975.1 529872..531305(-) (rpoN/rpoN1) [Kosakonia radicincitans strain DSM 107547]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQADLHEEVDSQQTQENEGLDTVDALEQKEMPE
ELPLDASWDEIYTAGTPSGSSHDYIDDELPIYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
VLESMGDEEIGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWCEEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYASMGGGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=363878 FEI17_RS02540 WP_035887975.1 529872..531305(-) (rpoN/rpoN1) [Kosakonia radicincitans strain DSM 107547]
ATGAAGCAAGGTTTGCAATTAAGGCTTAGCCAACAGCTAGCTATGACGCCGCAACTGCAACAGGCTATCCGTCTGTTGCA
GCTGTCTACGTTAGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAACCCATTGCTGGAACAAGCCGATCTTCATG
AAGAAGTGGATAGCCAACAAACCCAGGAAAATGAAGGCCTGGATACTGTAGACGCGCTCGAACAGAAAGAGATGCCTGAA
GAGCTGCCGCTGGACGCCAGCTGGGATGAGATTTATACCGCCGGAACGCCTTCCGGCAGCAGCCACGATTATATTGATGA
CGAACTGCCGATTTATCAGGGTGAAACGACACAGTCTCTTCAGGATTACCTGATGTGGCAGGTAGAGTTAACGCCGTTTT
CCGATACCGATCGCGCAATCGCAACCTCTATTGTCGATGCTGTCGATGAGACCGGCTACCTTACCGTGTCGCTTGAGGAT
GTGCTGGAAAGCATGGGCGATGAAGAAATCGGTCTCGATGAAGTTGAAGCGGTGTTAAAGCGCGTTCAGCGTTTCGATCC
GGTTGGCGTCGCTGCGCGCGATCTGCGCGACTGCCTGTTGATCCAACTGTCCCAATTCGCGAAAGATACGCCCTGGTGTG
AAGAGGCTCGCCTGATTATCAGCGATCACCTTGATCTGCTCGCTAACCATGATTTCCGCTCTCTCATGCGCGTCACACGT
CTTAAAGAGGATGTGCTGAAAGAAGCGGTAAATCTGATCCAGTCGCTGGATCCGCGCCCCGGCCAGTCGATCCAGACCGG
TGAACCAGAATATGTCATCCCGGATGTGCTGGTGCGTAAGCACAACGGGCGCTGGACGGTAGAACTGAACTCCGATAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCTTCAATGGGCGGCGGCGCGCGCAATGACGCCGACACCCAGTTTATCCGC
AGTAACTTGCAGGAAGCGAAGTGGCTGATCAAAAGCCTTGAAAGCCGTAATGACACGCTATTGCGCGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCATTCTTTGAACAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCTCAGGCCG
TCGAAATGCACGAGTCCACGATTTCCCGTGTGACAACGCAGAAGTACCTGCACAGTCCTCGCGGCATTTTTGAACTGAAG
TATTTCTTCTCCAGCCATGTGAATACGGAAGGCGGCGGCGAAGCCTCGTCAACAGCCATTCGCGCACTGGTTAAGAAATT
AATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACGACGCTACTTTCCGATCAAGGAATTATGGTAG
CGCGGCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.747

100

0.482

Multiple sequence alignment