Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FED53_RS20310 Genome accession   NZ_CP040367
Coordinates   3822488..3823867 (-) Length   459 a.a.
NCBI ID   WP_025909699.1    Uniprot ID   A0A0V8JL36
Organism   Priestia flexa isolate 1-2-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3817488..3828867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FED53_RS20290 (FED53_20295) ispF 3818959..3819438 (-) 480 WP_025909703.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  FED53_RS20295 (FED53_20300) ispD 3819451..3820140 (-) 690 WP_025909702.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  FED53_RS20300 (FED53_20305) - 3820166..3821263 (-) 1098 WP_061784782.1 PIN/TRAM domain-containing protein -
  FED53_RS20305 (FED53_20310) disA 3821406..3822482 (-) 1077 WP_078988977.1 DNA integrity scanning diadenylate cyclase DisA -
  FED53_RS20310 (FED53_20315) radA 3822488..3823867 (-) 1380 WP_025909699.1 DNA repair protein RadA Machinery gene
  FED53_RS20315 (FED53_20320) clpC 3823991..3826432 (-) 2442 WP_119542722.1 ATP-dependent protease ATP-binding subunit ClpC -
  FED53_RS20320 (FED53_20325) - 3826453..3827520 (-) 1068 WP_025909697.1 protein arginine kinase -
  FED53_RS20325 (FED53_20330) - 3827525..3828064 (-) 540 WP_025909696.1 UvrB/UvrC motif-containing protein -
  FED53_RS20330 (FED53_20335) - 3828102..3828563 (-) 462 WP_078988979.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49709.94 Da        Isoelectric Point: 7.4549

>NTDB_id=363718 FED53_RS20310 WP_025909699.1 3822488..3823867(-) (radA) [Priestia flexa isolate 1-2-1]
MSKRKTKFSCQHCGYESAKWMGKCPGCGSWNSMVEEIEETKSSRRGGAFSGATSTKVQKPQSITNIESTTEPRIYTKSPE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMNKHKVLYISGEESTKQTKLRADRLGIKAEDLYVHAETNLESI
TEAISSMNPELVIIDSIQTIYHGDVTSAPGSVSQVRECTAELMRIAKTNGIAIFIVGHVTKEGSIAGPRLLEHMVDSVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTS
FGNPRRMSTGLDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFKDAPSNPTDVVVGEVGL
TGEVRRVSRIEQRVQEAVKLGFKRIIVPEKNLGGWKIPEGIDVVGVSTVSEALQYTLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=363718 FED53_RS20310 WP_025909699.1 3822488..3823867(-) (radA) [Priestia flexa isolate 1-2-1]
ATGAGTAAAAGAAAAACAAAGTTTTCATGTCAACATTGTGGGTATGAATCGGCTAAATGGATGGGAAAGTGTCCTGGCTG
TGGCAGTTGGAATTCAATGGTGGAAGAAATTGAAGAGACAAAGTCTTCAAGGCGAGGAGGAGCTTTTTCTGGCGCAACGT
CTACAAAGGTGCAAAAGCCTCAATCCATTACGAATATTGAATCAACGACTGAACCAAGGATATATACAAAGTCACCTGAG
CTAAACCGTGTTTTAGGTGGAGGGATTGTTAGAGGATCACTAGTCTTAATTGGGGGAGATCCTGGTATTGGTAAATCAAC
TTTGTTGCTACAAACTTCAGCTCAGTTAGCTATGAACAAGCATAAGGTGCTTTATATATCTGGTGAGGAATCAACAAAGC
AAACAAAGCTAAGAGCAGATCGCTTAGGTATTAAAGCGGAAGATTTGTATGTGCATGCAGAAACAAACTTAGAGTCAATC
ACAGAAGCTATCTCAAGTATGAATCCAGAACTTGTTATCATTGATTCAATTCAAACAATTTATCACGGTGACGTTACATC
AGCGCCCGGAAGTGTTTCTCAAGTACGAGAGTGTACAGCAGAGTTAATGCGAATTGCAAAAACAAACGGCATAGCTATTT
TTATTGTAGGTCATGTTACCAAAGAAGGGTCTATTGCAGGTCCAAGGTTACTTGAACATATGGTAGACAGTGTTTTGTAT
TTTGAAGGAGAACGCCACCATACGTACCGGATTTTAAGAGCAGTAAAAAACCGCTTTGGTTCAACAAACGAAATGGGTAT
TTTTGAAATGAAGGAAAGTGGTCTAGAGGAAGTGCTCAACCCATCTGAAATCTTTTTAGAAGAACGATCTCAAGGTGCAG
CTGGTTCTGTGGTTGTGGCTTCGATGGAGGGTACTCGTCCCGTATTGGTAGAGTTACAAGCACTTATTAGCCCAACAAGC
TTTGGAAATCCTAGAAGAATGTCTACAGGACTTGATCATAACCGTGTCTCGCTTATTATGGCAGTGTTAGAAAAGCGAGT
AGGATTACTGTTGCAAAATCAAGATGCGTACTTAAAAGTAGCAGGTGGGGTAAAGTTAGATGAGCCAGCTATTGATTTGG
CGGTTGCGGTTAGCATTGCATCGAGTTTTAAAGATGCACCTTCAAACCCTACAGACGTAGTAGTAGGAGAAGTTGGATTA
ACAGGTGAGGTTCGAAGAGTGTCTCGCATTGAACAACGAGTGCAAGAAGCTGTGAAATTAGGATTTAAGCGAATCATTGT
TCCTGAAAAAAACTTAGGTGGTTGGAAAATACCAGAAGGCATTGACGTAGTAGGGGTGTCCACTGTGTCAGAAGCGTTAC
AGTACACGTTAGGAGGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0V8JL36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.381

100

0.754
  radA Streptococcus pneumoniae Rx1

63.216

98.911

0.625
  radA Streptococcus pneumoniae D39

63.216

98.911

0.625
  radA Streptococcus pneumoniae R6

63.216

98.911

0.625
  radA Streptococcus pneumoniae TIGR4

63.216

98.911

0.625
  radA Streptococcus mitis NCTC 12261

63.216

98.911

0.625
  radA Streptococcus mitis SK321

63.216

98.911

0.625

Multiple sequence alignment