Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   FED53_RS17175 Genome accession   NZ_CP040367
Coordinates   3223736..3225556 (-) Length   606 a.a.
NCBI ID   WP_078990186.1    Uniprot ID   A0A8I1MEV7
Organism   Priestia flexa isolate 1-2-1     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3159307..3223695 3223736..3225556 flank 41


Gene organization within MGE regions


Location: 3159307..3225556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FED53_RS20990 - 3159307..3159468 (-) 162 WP_175406267.1 hypothetical protein -
  FED53_RS20995 - 3159701..3159841 (+) 141 WP_160169854.1 hypothetical protein -
  FED53_RS21000 - 3160004..3160300 (-) 297 WP_076512601.1 hypothetical protein -
  FED53_RS16860 (FED53_16860) - 3160313..3161197 (-) 885 WP_175406268.1 hypothetical protein -
  FED53_RS16865 (FED53_16865) - 3162082..3163197 (+) 1116 WP_138116240.1 IS4 family transposase -
  FED53_RS16870 (FED53_16870) - 3163783..3164139 (+) 357 WP_025908862.1 hypothetical protein -
  FED53_RS21005 - 3164300..3164437 (-) 138 WP_100347981.1 hypothetical protein -
  FED53_RS16875 (FED53_16875) - 3164983..3165510 (-) 528 WP_235932366.1 hypothetical protein -
  FED53_RS16880 (FED53_16880) - 3165519..3166295 (-) 777 WP_138117339.1 SAR2788 family putative toxin -
  FED53_RS16885 (FED53_16885) - 3166292..3167173 (-) 882 WP_138117340.1 S8 family peptidase -
  FED53_RS16890 (FED53_16890) istA 3167379..3168641 (+) 1263 WP_240046365.1 IS21 family transposase -
  FED53_RS16895 (FED53_16895) istB 3168638..3169402 (+) 765 WP_000980839.1 IS21-like element helper ATPase IstB -
  FED53_RS16900 (FED53_16900) - 3169969..3171090 (+) 1122 Protein_3270 IS3 family transposase -
  FED53_RS16905 (FED53_16905) - 3171500..3173353 (-) 1854 WP_138117341.1 UvrD-helicase domain-containing protein -
  FED53_RS16910 (FED53_16910) - 3173374..3175425 (-) 2052 WP_138117342.1 ATP-dependent nuclease -
  FED53_RS16915 (FED53_16915) - 3175861..3179013 (-) 3153 WP_138117343.1 NACHT domain-containing protein -
  FED53_RS16920 (FED53_16920) - 3179025..3180245 (-) 1221 WP_138117344.1 MBL fold metallo-hydrolase -
  FED53_RS16925 (FED53_16925) - 3180842..3182000 (+) 1159 WP_275671961.1 IS3 family transposase -
  FED53_RS16930 (FED53_16930) - 3182169..3182384 (-) 216 WP_138117345.1 glycine zipper family protein -
  FED53_RS16935 (FED53_16935) - 3182696..3183202 (-) 507 WP_138117346.1 JAB domain-containing protein -
  FED53_RS16940 (FED53_16940) - 3183763..3184053 (-) 291 WP_138117347.1 hypothetical protein -
  FED53_RS16945 (FED53_16945) istB 3184143..3184907 (-) 765 WP_025909801.1 IS21-like element helper ATPase IstB -
  FED53_RS16950 (FED53_16950) - 3184910..3185569 (-) 660 WP_175406269.1 hypothetical protein -
  FED53_RS16955 (FED53_16955) - 3185617..3185808 (+) 192 WP_025910450.1 hypothetical protein -
  FED53_RS16960 (FED53_16960) tnpB 3185805..3186158 (+) 354 WP_025910451.1 IS66 family insertion sequence element accessory protein TnpB -
  FED53_RS16965 (FED53_16965) tnpC 3186221..3187774 (+) 1554 WP_420892550.1 IS66 family transposase -
  FED53_RS16970 (FED53_16970) istA 3187835..3188803 (-) 969 WP_240046404.1 IS21 family transposase -
  FED53_RS16975 (FED53_16975) - 3189042..3190127 (+) 1086 WP_240046405.1 site-specific integrase -
  FED53_RS16985 (FED53_16985) - 3190457..3191218 (+) 762 WP_025908653.1 arylamine N-acetyltransferase family protein -
  FED53_RS16990 (FED53_16990) - 3191276..3191857 (-) 582 WP_138117348.1 DUF1349 domain-containing protein -
  FED53_RS16995 (FED53_16995) - 3191934..3193259 (-) 1326 WP_061786020.1 GntP family permease -
  FED53_RS17000 (FED53_17000) gntK 3193786..3195327 (-) 1542 WP_138117349.1 gluconokinase -
  FED53_RS17005 (FED53_17005) gnd 3195349..3196245 (-) 897 WP_138117350.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  FED53_RS17010 (FED53_17010) - 3196394..3197239 (+) 846 WP_061786022.1 MurR/RpiR family transcriptional regulator -
  FED53_RS17015 (FED53_17015) - 3197397..3197957 (-) 561 WP_138117351.1 AAA family ATPase -
  FED53_RS17020 (FED53_17020) - 3198409..3198882 (+) 474 WP_025908646.1 GNAT family N-acetyltransferase -
  FED53_RS17025 (FED53_17025) - 3198925..3199467 (-) 543 WP_025908645.1 phosphatidylglycerophosphatase A -
  FED53_RS17030 (FED53_17030) - 3199672..3200073 (+) 402 WP_061786023.1 hypothetical protein -
  FED53_RS17035 (FED53_17035) - 3200165..3200890 (+) 726 WP_025908643.1 alpha/beta hydrolase -
  FED53_RS17040 (FED53_17040) - 3200973..3201485 (+) 513 WP_025908642.1 YjcG family protein -
  FED53_RS17045 (FED53_17045) - 3201492..3201914 (+) 423 WP_025908641.1 GNAT family N-acetyltransferase -
  FED53_RS17050 (FED53_17050) - 3202013..3203312 (-) 1300 Protein_3299 spore germination protein -
  FED53_RS17060 (FED53_17060) - 3203795..3204472 (+) 678 WP_025908636.1 DUF421 domain-containing protein -
  FED53_RS17065 (FED53_17065) - 3204535..3204777 (-) 243 WP_061785614.1 hypothetical protein -
  FED53_RS17070 (FED53_17070) - 3204941..3207250 (+) 2310 WP_138117353.1 UvrD-helicase domain-containing protein -
  FED53_RS17075 (FED53_17075) - 3207292..3207552 (-) 261 WP_025908120.1 stage VI sporulation protein F -
  FED53_RS17080 (FED53_17080) - 3207772..3207891 (-) 120 WP_076512614.1 YjcZ family sporulation protein -
  FED53_RS17085 (FED53_17085) - 3207974..3208153 (-) 180 WP_025908121.1 hypothetical protein -
  FED53_RS21010 - 3208224..3208382 (-) 159 WP_167556173.1 hypothetical protein -
  FED53_RS17090 (FED53_17090) - 3208453..3208902 (-) 450 WP_025908122.1 hypothetical protein -
  FED53_RS17095 (FED53_17095) - 3209154..3209918 (+) 765 WP_138117354.1 hypothetical protein -
  FED53_RS17100 (FED53_17100) - 3210023..3210883 (+) 861 WP_100348002.1 hypothetical protein -
  FED53_RS17105 (FED53_17105) - 3210931..3211266 (-) 336 WP_100348003.1 DUF1360 domain-containing protein -
  FED53_RS17110 (FED53_17110) - 3211330..3211803 (-) 474 WP_025908126.1 CotO family spore coat protein -
  FED53_RS17115 (FED53_17115) fabI 3211933..3212715 (-) 783 WP_025908127.1 enoyl-ACP reductase FabI -
  FED53_RS17120 (FED53_17120) - 3213049..3214908 (-) 1860 WP_025908128.1 monovalent cation:proton antiporter family protein -
  FED53_RS17125 (FED53_17125) mgtE 3215018..3216394 (-) 1377 WP_025908129.1 magnesium transporter -
  FED53_RS17130 (FED53_17130) prpE 3216616..3217350 (+) 735 WP_138117355.1 bis(5'-nucleosyl)-tetraphosphatase PrpE -
  FED53_RS17135 (FED53_17135) - 3217389..3218291 (-) 903 WP_100348006.1 RluA family pseudouridine synthase -
  FED53_RS17140 (FED53_17140) - 3218307..3219101 (-) 795 WP_061785629.1 NAD kinase -
  FED53_RS17145 (FED53_17145) - 3219160..3219801 (-) 642 WP_025908133.1 GTP pyrophosphokinase -
  FED53_RS17150 (FED53_17150) - 3219821..3220207 (-) 387 WP_025908134.1 hypothetical protein -
  FED53_RS17155 (FED53_17155) - 3220511..3221101 (+) 591 WP_076513368.1 CYTH domain-containing protein -
  FED53_RS17160 (FED53_17160) - 3221106..3221750 (+) 645 WP_025908136.1 lytic transglycosylase domain-containing protein -
  FED53_RS17165 (FED53_17165) - 3221971..3222381 (+) 411 WP_061785625.1 globin -
  FED53_RS17170 (FED53_17170) - 3222378..3223262 (+) 885 WP_061785626.1 ClpXP adapter SpxH family protein -
  FED53_RS21015 - 3223519..3223695 (+) 177 WP_090752849.1 hypothetical protein -
  FED53_RS17175 (FED53_17175) pepF 3223736..3225556 (-) 1821 WP_078990186.1 oligoendopeptidase F Regulator

Sequence

Protein


Download         Length: 606 a.a.        Molecular weight: 70113.76 Da        Isoelectric Point: 4.6532

>NTDB_id=363708 FED53_RS17175 WP_078990186.1 3223736..3225556(-) (pepF) [Priestia flexa isolate 1-2-1]
MSEQSTVKKLPNRSEIKVEDTWRLEDIFETDEAWEKEFQAVKALVPEMEQYRGKLGESADMLFKALQQQDELTMRVSKLY
TYAHMRYDQDTTNSFYQGLNDRIKMLYTEIASALSYVTPELLAVDEETLKQYLKDSKDLQLYAHALDEVNRERPHILSES
EEAILAQASEALGASSNTFGMLNNADLEFPSIKDENGEEVEITHGRYIRFLESSDRRVREEAFKAVYDTYGKFKNTFAST
LSGTVKKDNFSAKVRNYESARHAALSANNIPEEVYDNLVATVNEYLPLLQRYIDLRKKVLGVDELHMYDLYTPLVQDVKM
EIPYSEAKDYLLKGLAPLGEEYISILKEGFENRWVDVHENKGKRSGAYSSGTYGTNPYILMNWQDNVNNLFTLAHEFGHS
VHSYYTRKEQPYPYGDYSIFVAEVASTCNEALLNDYLLKTIDDEKKRLYLLNHYLEGFRGTVFRQTMFAEFEHEIHVRAQ
NGEPLTPELLTKLYYDLNKKYFGDNLVIDEEIGLEWARIPHFYYNYYVYQYATGFSAAAALSKQILEEGDAAVERYLGFL
KAGSSDYPIEVLKRAGVDMTTAEPIKEALEVFKEKLEEMERLLEKN

Nucleotide


Download         Length: 1821 bp        

>NTDB_id=363708 FED53_RS17175 WP_078990186.1 3223736..3225556(-) (pepF) [Priestia flexa isolate 1-2-1]
ATGAGTGAACAATCCACGGTGAAAAAGCTGCCAAATCGCAGTGAAATTAAAGTAGAAGATACATGGAGATTAGAAGATAT
TTTTGAAACAGATGAAGCGTGGGAGAAAGAATTTCAAGCTGTTAAAGCCTTAGTTCCGGAAATGGAACAATATCGCGGCA
AGCTTGGAGAATCAGCAGATATGCTTTTTAAAGCACTTCAGCAGCAAGATGAACTAACAATGCGGGTCAGCAAGCTTTAT
ACATATGCACATATGAGGTATGACCAAGATACAACGAACTCATTCTATCAAGGATTAAATGATCGAATTAAAATGTTGTA
TACAGAAATTGCGAGTGCGTTATCTTACGTCACGCCTGAACTATTAGCAGTAGATGAGGAAACGCTAAAACAATATTTAA
AAGATAGCAAGGATTTACAGCTATATGCACATGCGCTGGATGAAGTGAATCGTGAGCGTCCTCATATCCTTTCTGAGAGT
GAAGAAGCTATTCTAGCACAGGCATCTGAAGCGCTAGGAGCATCAAGTAATACGTTCGGTATGCTGAATAATGCTGATTT
AGAGTTTCCATCTATTAAAGATGAAAACGGGGAAGAAGTGGAGATTACCCACGGTCGTTATATTCGGTTTTTAGAAAGCA
GTGATCGACGCGTTAGAGAAGAAGCGTTTAAAGCCGTCTATGATACGTATGGAAAGTTTAAAAATACATTTGCGAGTACT
CTTAGCGGAACGGTTAAAAAAGATAACTTTAGCGCTAAGGTCCGTAACTACGAATCAGCTCGCCATGCAGCTCTAAGCGC
AAACAATATTCCAGAAGAGGTATATGATAATTTAGTTGCAACGGTAAACGAATATCTGCCTCTATTACAGCGCTATATTG
ACCTACGAAAGAAAGTGTTAGGTGTTGATGAGCTTCACATGTATGATCTATATACACCGCTTGTGCAAGACGTCAAAATG
GAAATACCATATAGCGAAGCAAAAGATTATTTATTAAAAGGTTTGGCGCCGCTTGGAGAAGAGTACATTTCCATTTTAAA
AGAAGGATTTGAAAATCGCTGGGTTGATGTGCACGAAAACAAAGGAAAGCGAAGCGGTGCTTATTCTTCTGGAACATATG
GAACAAATCCATATATCTTAATGAACTGGCAGGACAATGTGAACAATCTTTTCACATTAGCTCATGAGTTTGGTCACTCA
GTGCACAGTTACTATACGCGTAAAGAACAGCCTTACCCATACGGTGATTATTCAATCTTTGTCGCTGAAGTCGCATCTAC
GTGTAACGAAGCTCTATTAAATGATTATTTATTGAAAACAATCGATGATGAAAAGAAACGATTATACTTATTAAATCATT
ATTTAGAAGGCTTCCGCGGTACCGTGTTCCGTCAAACGATGTTTGCTGAATTTGAGCATGAAATTCACGTTCGGGCTCAA
AATGGTGAACCGCTAACACCAGAGCTGCTAACAAAGTTATACTATGATTTAAATAAAAAGTACTTTGGTGATAATTTAGT
CATTGATGAAGAGATTGGCTTAGAGTGGGCAAGAATTCCACATTTCTACTATAATTATTATGTGTATCAATACGCAACAG
GCTTCAGCGCAGCTGCAGCGCTAAGTAAGCAAATTCTTGAAGAAGGAGACGCGGCTGTTGAGCGTTACCTTGGCTTCTTA
AAAGCTGGAAGCTCTGATTATCCAATCGAAGTGCTAAAACGCGCAGGAGTGGATATGACAACTGCTGAACCAATCAAAGA
AGCATTAGAAGTGTTTAAAGAAAAGCTTGAAGAGATGGAACGTTTGTTAGAGAAAAACTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.327

98.02

0.484

Multiple sequence alignment