Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   FED53_RS04055 Genome accession   NZ_CP040367
Coordinates   670278..672620 (+) Length   780 a.a.
NCBI ID   WP_138116603.1    Uniprot ID   -
Organism   Priestia flexa isolate 1-2-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 665278..677620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FED53_RS04040 (FED53_04040) - 666535..666828 (+) 294 WP_025911741.1 post-transcriptional regulator -
  FED53_RS04045 (FED53_04045) secDF 666942..669176 (+) 2235 WP_138116601.1 protein translocase subunit SecDF -
  FED53_RS04050 (FED53_04050) - 669313..670206 (+) 894 WP_025911738.1 cation diffusion facilitator family transporter -
  FED53_RS04055 (FED53_04055) recJ 670278..672620 (+) 2343 WP_138116603.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  FED53_RS04060 (FED53_04060) - 672636..673148 (+) 513 WP_076512678.1 adenine phosphoribosyltransferase -
  FED53_RS04065 (FED53_04065) - 673643..675841 (+) 2199 WP_025911733.1 RelA/SpoT family protein -
  FED53_RS04070 (FED53_04070) dtd 675858..676304 (+) 447 WP_138116605.1 D-aminoacyl-tRNA deacylase -
  FED53_RS04075 (FED53_04075) - 676352..677305 (-) 954 WP_083686434.1 N-acetylmuramoyl-L-alanine amidase -
  FED53_RS04080 (FED53_04080) - 677387..677563 (+) 177 WP_035321387.1 hypothetical protein -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87428.02 Da        Isoelectric Point: 4.7392

>NTDB_id=363683 FED53_RS04055 WP_138116603.1 670278..672620(+) (recJ) [Priestia flexa isolate 1-2-1]
MLQSKKRWNVKHIDDPLVDEFAKKLNITPLVASLLINRGIQTVEEASQFLHIEDESFHDPFLLEGMEETVARIRQAIENN
ERIMIYGDYDADGVSSTSVMLSVLGQLGAQVDFYIPNRFTEGYGPNEQAFRQIGDEGYGLVITVDTGISAVKEAKLAAEI
GFDLIITDHHEPGPEIPEAYAIIHPKLPGGGYPFKELAGVGVAFKLAHALLGEAPEHLLELAVIGTIADLVPLVGENRLI
AKKGLRYLKSSTRPGVEALLKVCSVKKEDITEDTIGFALAPRINAVGRLQDANPAVDLLMSTHYEEAIELAKEIDALNKE
RQQIVNEITQEAIDTVESLYPPSENAVLIIEGEGWNAGVVGIVASRLVEKFYRPTIVLSLDHETGLAKGSARSIAGFDLF
ANLSKSRDILPHFGGHPMAAGMTLEAKNVELLRQRLNEQAREVLTEQDFIPITSIDTICKVEDITIAGIEELQTLAPFGM
HNPKPKVLVERVTPTSMRKIGSDKNHLKLVVEDNGLSIDVVGFGLGHVLDEVSPLAKVSLVGELSINEWNNIRKPQLMIE
DVAVSEWQLFDYRNSFDMKRFFQEIFTKEMDIVTFNQQTVQKHATILPMDQVVEISTDEQASLYNSADKQMILLDLPDSK
ERLNLLFAEGKPARIYAMFVTENDSYFDTIPTREHFKWFYGLLAKRSPFDLKHYKEEIAIHKGWTKDTIDFICQVFFELE
FVTINNGLISLVKNSPKRDLIESVTYRKKQQQIEMEKNFIYSSYAQLKSEFEKLLASADE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=363683 FED53_RS04055 WP_138116603.1 670278..672620(+) (recJ) [Priestia flexa isolate 1-2-1]
ATGCTACAGTCAAAGAAGCGTTGGAATGTAAAACATATAGATGATCCACTTGTTGATGAATTTGCAAAGAAACTCAATAT
TACACCTCTTGTTGCTTCACTGCTTATTAATCGAGGAATTCAAACAGTAGAAGAAGCCTCACAGTTTTTACATATTGAGG
ATGAATCTTTTCATGACCCATTTCTTTTAGAAGGTATGGAAGAAACGGTTGCGCGCATTCGTCAAGCGATTGAAAACAAT
GAACGTATTATGATATATGGGGATTATGATGCTGACGGTGTAAGCAGTACGTCCGTTATGCTTTCTGTTTTAGGTCAACT
CGGAGCGCAAGTTGATTTTTATATACCCAATCGTTTTACAGAAGGCTACGGCCCAAACGAACAGGCATTTAGACAGATTG
GTGATGAAGGATATGGATTAGTTATTACAGTAGATACTGGAATTTCCGCTGTTAAAGAGGCAAAGCTAGCAGCTGAGATA
GGGTTTGATTTAATCATTACAGACCACCATGAACCAGGTCCAGAAATTCCGGAAGCCTATGCGATTATCCATCCTAAACT
TCCAGGTGGAGGCTATCCATTCAAAGAGCTAGCTGGAGTTGGAGTAGCGTTTAAATTAGCTCACGCTTTGCTCGGTGAAG
CTCCTGAACATTTATTGGAGCTAGCTGTCATTGGTACAATTGCTGATTTGGTGCCGCTAGTAGGAGAAAATCGACTAATA
GCGAAAAAGGGCTTACGCTATTTAAAAAGTTCAACTCGACCTGGAGTTGAAGCGCTATTAAAAGTGTGCAGTGTGAAGAA
AGAAGACATTACGGAAGACACAATTGGTTTTGCCCTTGCTCCAAGAATTAATGCTGTAGGTCGTCTTCAAGATGCGAACC
CGGCAGTCGATTTACTTATGTCTACTCATTATGAAGAAGCAATCGAGTTAGCAAAAGAAATTGATGCTTTAAATAAAGAG
CGCCAACAGATTGTAAATGAAATTACGCAAGAAGCAATCGATACTGTAGAAAGCCTATATCCGCCAAGTGAAAATGCAGT
CTTAATTATTGAAGGTGAAGGGTGGAACGCAGGAGTTGTTGGAATCGTAGCTTCTCGGTTGGTTGAAAAGTTCTATCGTC
CAACTATTGTGTTAAGTTTGGATCACGAAACCGGCTTAGCAAAAGGGTCGGCAAGAAGCATTGCTGGATTTGATTTATTT
GCAAACTTATCAAAGAGTCGTGATATTTTACCGCATTTCGGGGGCCACCCGATGGCTGCAGGGATGACGCTTGAAGCGAA
GAATGTTGAGCTACTGCGCCAGCGTTTAAATGAACAAGCGCGAGAAGTGCTAACTGAACAAGACTTCATTCCGATTACCT
CGATTGATACGATATGCAAAGTTGAGGATATTACAATCGCTGGTATTGAAGAGCTCCAAACACTCGCTCCTTTTGGCATG
CATAACCCCAAGCCGAAGGTGCTTGTTGAGCGTGTTACGCCAACATCAATGCGTAAAATCGGCAGTGATAAAAACCATTT
AAAGCTTGTTGTAGAAGATAATGGGCTATCAATTGACGTCGTAGGGTTTGGTTTAGGACATGTATTAGATGAAGTCTCTC
CGTTAGCCAAAGTATCGTTAGTCGGTGAGCTTTCAATTAACGAATGGAATAATATACGCAAGCCACAGCTCATGATTGAA
GATGTAGCAGTGAGTGAATGGCAGCTTTTTGATTATCGTAATTCATTTGATATGAAGAGATTCTTTCAAGAAATTTTCAC
GAAAGAAATGGATATCGTGACGTTTAATCAACAAACCGTTCAAAAGCATGCAACGATTTTGCCGATGGATCAAGTCGTTG
AGATTTCGACAGATGAACAAGCTAGTCTATATAATTCAGCTGATAAACAAATGATTCTTTTAGATTTACCAGATTCAAAA
GAGAGGTTAAATCTCTTATTTGCTGAAGGAAAGCCGGCTCGAATTTATGCGATGTTCGTAACAGAAAATGATAGTTATTT
TGATACGATTCCGACTCGTGAACATTTTAAGTGGTTTTATGGACTGCTGGCTAAAAGAAGCCCGTTTGATTTAAAACACT
ATAAAGAAGAGATTGCCATTCATAAAGGCTGGACAAAAGATACAATTGATTTTATCTGCCAGGTGTTTTTCGAACTAGAA
TTTGTTACAATAAACAATGGTCTAATTTCGCTAGTGAAAAATAGTCCTAAACGTGATTTAATAGAGTCTGTGACTTATCG
CAAGAAACAGCAACAAATTGAAATGGAAAAAAACTTTATTTATAGTTCATATGCACAGTTAAAAAGTGAGTTTGAGAAGC
TTCTTGCAAGTGCGGATGAATGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.846

100

0.538

Multiple sequence alignment