Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EQK03_RS13085 Genome accession   NZ_CP040342
Coordinates   2545024..2545458 (+) Length   144 a.a.
NCBI ID   WP_000811511.1    Uniprot ID   -
Organism   Bacillus cereus strain DLOU-Weihai     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2540024..2550458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQK03_RS13065 - 2540938..2542101 (-) 1164 WP_000434577.1 aminotransferase A -
  EQK03_RS13070 - 2542224..2542424 (-) 201 WP_000929280.1 hypothetical protein -
  EQK03_RS13075 kinB 2542735..2544009 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  EQK03_RS13080 - 2544048..2544797 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  EQK03_RS13085 nucA/comI 2545024..2545458 (+) 435 WP_000811511.1 DNA-entry nuclease Machinery gene
  EQK03_RS13090 comJ 2545495..2545884 (+) 390 WP_000424030.1 competence protein ComJ -
  EQK03_RS13095 metE 2545923..2548211 (-) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EQK03_RS13100 - 2548724..2549281 (-) 558 WP_000062062.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16082.49 Da        Isoelectric Point: 8.4331

>NTDB_id=363466 EQK03_RS13085 WP_000811511.1 2545024..2545458(+) (nucA/comI) [Bacillus cereus strain DLOU-Weihai]
MKQLKGIIISIIVILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=363466 EQK03_RS13085 WP_000811511.1 2545024..2545458(+) (nucA/comI) [Bacillus cereus strain DLOU-Weihai]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGTAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment