Detailed information NTG0363372

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: EUC40_RS25385
• Genome accession: NZ_CP040336
• Coordinates: 5020596..5021972 (-)
• Length: 458 a.a.
• NCBI ID: WP_060629200.1
• Uniprot ID: A0A2B1I2Z9
• Organism: Bacillus luti strain FJ
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 5015596..5026972

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EUC40_RS25365	ispF	5016959..5017435 (-)	477	WP_156576854.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
EUC40_RS25370	ispD	5017552..5018232 (-)	681	WP_156576855.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
EUC40_RS25375	-	5018249..5019358 (-)	1110	WP_000919690.1	PIN/TRAM domain-containing protein	-
EUC40_RS25380	disA	5019519..5020592 (-)	1074	WP_000392167.1	DNA integrity scanning diadenylate cyclase DisA	-
EUC40_RS25385	radA	5020596..5021972 (-)	1377	WP_060629200.1	DNA repair protein RadA	Machinery gene
EUC40_RS25390	clpC	5022069..5024504 (-)	2436	WP_000971178.1	ATP-dependent protease ATP-binding subunit ClpC	Regulator
EUC40_RS25395	-	5024527..5025591 (-)	1065	WP_071711520.1	protein arginine kinase	-
EUC40_RS25400	-	5025596..5026144 (-)	549	WP_156576856.1	UvrB/UvrC motif-containing protein	-
EUC40_RS25405	ctsR	5026315..5026776 (-)	462	WP_001244562.1	transcriptional regulator CtsR	-

Sequence

Protein

• Length: 458 a.a.
• Molecular weight: 49866.52 Da
• Isoelectric Point: 7.0904

>NTDB_id=363372 EUC40_RS25385 WP_060629200.1 5020596..5021972(-) (radA) [Bacillus luti strain FJ]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLDVKGSNLFVVAETDLQRIA
THIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide

• Length: 1377 bp

>NTDB_id=363372 EUC40_RS25385 WP_060629200.1 5020596..5021972(-) (radA) [Bacillus luti strain FJ]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGCGGTTATCAGTCACCAAAATATATGGGTAAATGCCCTGGATG
TGGTCAATGGAATACACTTGTTGAAGAAATGGAACCGGTTGTATCATCCAGACGCCTTAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGTGGAATCGTAGATGGATCTTTAGTACTTATCGGCGGGGACCCTGGGATTGGAAAATCCACGTT
GTTATTACAGATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTCTACATATCGGGTGAGGAGTCAGCAAAGCAGA
TAAAACTTCGTGCAGATCGTTTGGATGTAAAGGGTAGTAATTTATTTGTTGTAGCAGAGACGGATTTGCAGCGAATTGCA
ACACACATTGAAGAGATGAATCCAGCCTTTGTTGTTATTGATTCCATTCAAACGATACATTTACCAGAGGTGACATCAGC
GCCAGGAAGCGTGGCACAAGTACGTGAATGTACGGCAGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTCGGACACGTGACAAAAGAAGGTGCAATTGCAGGACCGCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATATCGTATTTTACGAGCTGTGAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCGGAGGTCTTAAACCCGTCAGAAATTTTCCTTGAGGAAAGACCGGTTGGGGTCGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCGACGGGAATTGACCATAACCGTGTATCGCTTATTATGGCAGTGTTAGAAAAAAGAACAGG
TTTATTATTGCAAAATCAAGATGCATATTTAAAAGTAGCAGGTGGTTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TAGCTTTGAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGGTTCCAACGAGCTATTATTCC
TAGAAAAAATTTGGGAGGGTGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (363-430)

• AAA domain (AAA_25) (60-222)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

3D structure

• AlphaFold DB: A0A2B1I2Z9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	74.728	100	0.749
radA	Streptococcus mitis NCTC 12261	64.018	98.908	0.633
radA	Streptococcus pneumoniae Rx1	63.797	98.908	0.631
radA	Streptococcus pneumoniae D39	63.797	98.908	0.631
radA	Streptococcus pneumoniae R6	63.797	98.908	0.631
radA	Streptococcus pneumoniae TIGR4	63.797	98.908	0.631
radA	Streptococcus mitis SK321	63.797	98.908	0.631