Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EUC40_RS20165 Genome accession   NZ_CP040336
Coordinates   3966590..3970315 (-) Length   1241 a.a.
NCBI ID   WP_156576376.1    Uniprot ID   -
Organism   Bacillus luti strain FJ     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3961590..3975315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUC40_RS20120 - 3961844..3962458 (+) 615 WP_001070758.1 spore germination protein GerPC -
  EUC40_RS20125 gerPD 3962465..3962659 (+) 195 WP_001102342.1 spore germination protein GerPD -
  EUC40_RS20130 - 3962675..3963061 (+) 387 WP_149175974.1 spore germination protein GerPE -
  EUC40_RS20135 gerPF 3963104..3963319 (+) 216 WP_001141570.1 spore germination protein GerPF -
  EUC40_RS20140 - 3963584..3963964 (-) 381 WP_071711096.1 hypothetical protein -
  EUC40_RS20145 - 3963975..3965144 (-) 1170 WP_156576372.1 hypothetical protein -
  EUC40_RS20150 - 3965203..3965580 (+) 378 WP_156576373.1 DUF1433 domain-containing protein -
  EUC40_RS20155 - 3965817..3966194 (+) 378 WP_156576374.1 DUF1433 domain-containing protein -
  EUC40_RS20160 - 3966288..3966566 (-) 279 WP_156576375.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EUC40_RS20165 addA 3966590..3970315 (-) 3726 WP_156576376.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EUC40_RS20170 addB 3970312..3973827 (-) 3516 WP_156576377.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EUC40_RS20175 lepB 3973944..3974507 (-) 564 WP_000751903.1 signal peptidase I -
  EUC40_RS20180 - 3974564..3975157 (-) 594 WP_000347509.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142770.00 Da        Isoelectric Point: 4.8172

>NTDB_id=363365 EUC40_RS20165 WP_156576376.1 3966590..3970315(-) (addA) [Bacillus luti strain FJ]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPVPRVETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPIGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYEYDTNWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLLWKYGYEEATSHRAKQSVTEIKRNYQSEDGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITAETLQEQIAAMVNKELLTFEQAEEMAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEEAILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=363365 EUC40_RS20165 WP_156576376.1 3966590..3970315(-) (addA) [Bacillus luti strain FJ]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGATCAGTGGAAAGCCGTTGTAGCGAACGGGCG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAACG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATC
GGAGAAGCATTAGAAAAAGTATTAATTGATGAACCAGGTTCGCAGCACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACGATTCACTCATTTTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTAGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAACTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCGCATCCGAATCCCGAGAAATGGCTTGATAAATTAGTAGAAGCATACGACGTCGAAGGAA
AGACCATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAGTTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACGGAACTCGCGATGCTTCCTGACGGTCCAGTGCCTCGCGTTGAAACTCTGCAAGCAGACGTAGCTTTACT
TGGAACGTTATCATCCGCTGCTCGTGAATCGTGGACAAGCGTATATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AACGTATTAAGAAAAGTGATTATAACGAAGATATTGTCAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGTAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTTCAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAGCAAAGTGAAAACGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTGTTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCGATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGCTTATTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGGCGGCGGAATGAAGATAGACTTAGCGAAA
AATTTCCGTAGTCGCCATGAAGTGCTAGCAGGAACGAACTTCATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAATTGGTGAAGATGTAGCGGCTGAACTATTATGCA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGAGAAGAAGGTGCAGAAGTCGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAGGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGCCCGGTACAATA
CCGTGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTGGGACTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCATGGGAAAAAAGGGTCATTTTATGAAGTAATGAGCTCGTTCTTAAAAGGAGCACCGCTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAATCCCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGTGGTTTACCAGCAGGAAAGCAAAGACAAGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCAAGAGCTCTAGGTGAACAAGAAGACGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTTGGTCGTCGTTTTAATACACAAGACTTAATGAA
GCGCTTCTTACTCCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGTATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGAGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCCCCTTCATTATATAGGC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAATATTCCAGGTGAAATTTATGAGTATGATACGAACTGGAAA
GTGGAGGTTGTTGACGGTAACACGTTACTTGCGCCAGAACCAGTTCAAGAAGAGAAACAAGAATTATTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGAAAAGAAGAAGTGTATGACAGATTACTGTGGAAGTACGGATATGAGG
AAGCAACCTCTCACCGTGCGAAGCAATCTGTTACAGAAATAAAAAGAAACTATCAATCAGAAGATGGTAGTGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACGCGTCCTCGTTTCATGGAGAAAAAAGGATTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCGATTACGGCCGAAACGCTACAAGAACAAATCGCAG
CAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCAGAAGAAATGGCAATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCAAAAAGTGTAGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGAAAGAGCGAAGAAGCAATCCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTTAAAACGGATACGATTGAAGGAAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAGGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGGAATCACGTTGTGAATATTGAGGAATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.087

100

0.533

Multiple sequence alignment