Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EUC40_RS07045 Genome accession   NZ_CP040336
Coordinates   1341697..1342131 (+) Length   144 a.a.
NCBI ID   WP_071713153.1    Uniprot ID   A0A7V7V641
Organism   Bacillus luti strain FJ     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1336697..1347131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUC40_RS07025 - 1337597..1338760 (-) 1164 WP_000434551.1 aminotransferase A -
  EUC40_RS07030 - 1338882..1339082 (-) 201 WP_000929277.1 hypothetical protein -
  EUC40_RS07035 kinB 1339393..1340667 (+) 1275 WP_071713151.1 sporulation sensor histidine kinase KinB -
  EUC40_RS07040 - 1340711..1341460 (-) 750 WP_156574229.1 DUF3967 domain-containing protein -
  EUC40_RS07045 nucA/comI 1341697..1342131 (+) 435 WP_071713153.1 DNA-entry nuclease Machinery gene
  EUC40_RS07050 comJ 1342167..1342556 (+) 390 WP_000424011.1 competence protein ComJ -
  EUC40_RS07055 metE 1342595..1344883 (-) 2289 WP_071713155.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EUC40_RS07060 - 1345406..1345963 (-) 558 WP_000062056.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16009.39 Da        Isoelectric Point: 8.9015

>NTDB_id=363346 EUC40_RS07045 WP_071713153.1 1341697..1342131(+) (nucA/comI) [Bacillus luti strain FJ]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITNAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=363346 EUC40_RS07045 WP_071713153.1 1341697..1342131(+) (nucA/comI) [Bacillus luti strain FJ]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGAATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATTGACCGCGGTGGTGCTGCAGATAGAAGAAAA
TTATCGTTAGCACCATATCCATCAAAAAAAGGGTATGACCGCGATGAATGGCCAATGGCAATGTGTAAAGAGGGCGGCAA
AGGAGCCCATATTGAATATATAAGTCCAGCAGATAACCGTGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V7V641

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.27

77.083

0.542

Multiple sequence alignment