Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FD735_RS00135 Genome accession   NZ_CP040231
Coordinates   23161..24498 (+) Length   445 a.a.
NCBI ID   WP_255296287.1    Uniprot ID   -
Organism   Streptococcus sp. 1643     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 18161..29498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FD735_RS09840 (FD735_00105) comW 19540..19776 (+) 237 WP_070567919.1 sigma(X)-activator ComW -
  FD735_RS00110 (FD735_00110) - 20018..21304 (+) 1287 WP_049506397.1 adenylosuccinate synthase -
  FD735_RS00115 (FD735_00115) tadA 21505..21972 (+) 468 WP_070567921.1 tRNA adenosine(34) deaminase TadA -
  FD735_RS00125 (FD735_00125) - 22163..22606 (+) 444 WP_139658197.1 dUTP diphosphatase -
  FD735_RS00130 (FD735_00130) - 22566..23123 (+) 558 WP_176553040.1 histidine phosphatase family protein -
  FD735_RS00135 (FD735_00135) radA 23161..24498 (+) 1338 WP_255296287.1 DNA repair protein RadA Machinery gene
  FD735_RS00140 (FD735_00140) - 24571..25065 (+) 495 WP_002876632.1 carbonic anhydrase -
  FD735_RS00145 (FD735_00145) - 25279..26247 (+) 969 WP_000010174.1 ribose-phosphate diphosphokinase -
  FD735_RS00150 (FD735_00150) abpA 26588..27235 (+) 648 WP_139658198.1 amylase-binding adhesin AbpA -
  FD735_RS00155 (FD735_00155) srtB 27329..28162 (+) 834 WP_139658199.1 class B sortase, LPKTxAVK-specific -
  FD735_RS00160 (FD735_00160) - 28280..28717 (+) 438 WP_000076495.1 CoA-binding protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 48601.83 Da        Isoelectric Point: 5.2532

>NTDB_id=362609 FD735_RS00135 WP_255296287.1 23161..24498(+) (radA) [Streptococcus sp. 1643]
MCQNCEYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPG
SLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVERIQPDFLI
VDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGERHHTFRILR
AVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDF
NRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIRRVNRIEQR
INEAAKLGFTKIYVPKNSLTGITPPKEIEVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=362609 FD735_RS00135 WP_255296287.1 23161..24498(+) (radA) [Streptococcus sp. 1643]
GTGTGTCAAAATTGTGAATATAATTCACCTAAGTATCTAGGGCGTTGTCCTAACTGTGGGTCTTGGTCTTCTTTTGTAGA
AGAGGTTGAGGTTGCCGAGGTCAAGAATGCACGAGTGTCCTTGACAGGTGAAAAAACCAAGCCTATGAAACTGGCTGAGG
TGACTTCCATCAATGTCAATCGAACCAAGACAGAGATGGAGGAATTCAACCGTGTGCTTGGTGGAGGTGTGGTACCGGGG
AGTCTCGTTCTTATCGGTGGAGATCCAGGAATCGGGAAATCAACCCTTCTCCTACAAGTTTCAACGCAGCTGTCTCAAGT
GGGTACGGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAGTTACGGGCAGAGCGCTTGGGAGATATTGATA
GTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGCGCAGAAGTAGAAAGAATTCAGCCAGACTTCCTCATT
GTTGACTCTATCCAGACGATTATGTCTCCTGAGATTTCAGGGGTGCAAGGGTCTGTGTCTCAGGTGCGTGAGGTGACCGC
TGAGCTTATGCAGCTGGCTAAGACCAATAACATTGCCATCTTTATCGTAGGGCATGTGACCAAGGAAGGGACCTTGGCTG
GTCCGCGTATGCTGGAGCATATGGTAGATACAGTGCTTTACTTTGAAGGGGAACGTCACCATACCTTTCGTATCTTGAGA
GCAGTCAAAAACCGTTTTGGTTCCACTAATGAGATTGGCATATTTGAGATGCAGTCGGGCGGATTGGTTGAGGTGCTCAA
TCCGAGTCAAGTTTTCCTAGAGGAGCGTTTGGATGGGGCTACTGGCTCGTCAATCGTTGTGACCATGGAAGGGACCCGTC
CGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCAACCATGTTTGGAAATGCTAAACGCACGACGACAGGACTTGATTTC
AATCGTGCAAGTCTGATTATGGCTGTTTTGGAAAAACGAGCAGGGCTTCTCTTGCAAAATCAGGATGCCTATCTCAAATC
TGCTGGTGGCGTGAAATTGGATGAGCCTGCCATTGACTTAGCCGTTGCAGTGGCTATTGCCTCTAGTTACAAGGACAAGC
CAACCAATCCTCAGGAATGTTTTGTGGGAGAATTGGGCTTGACTGGAGAGATTCGGCGCGTGAATCGTATCGAACAACGC
ATCAATGAAGCGGCAAAATTAGGCTTTACCAAGATTTATGTACCCAAGAATTCCTTGACAGGAATCACTCCACCCAAGGA
AATTGAAGTCATTGGTGTGACAACGATTCAAGAAGTTTTGAAAAAGGTCTTTGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(55-210)

Subunit ChlI of Mg-chelatase (ChlI)

(352-417)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

98.876

100

0.989
  radA Streptococcus mitis SK321

98.876

100

0.989
  radA Streptococcus pneumoniae Rx1

98.876

100

0.989
  radA Streptococcus pneumoniae D39

98.876

100

0.989
  radA Streptococcus pneumoniae R6

98.876

100

0.989
  radA Streptococcus pneumoniae TIGR4

98.876

100

0.989
  radA Bacillus subtilis subsp. subtilis str. 168

63.371

100

0.634

Multiple sequence alignment