Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   FAY22_RS07310 Genome accession   NZ_CP040128
Coordinates   1558601..1559971 (+) Length   456 a.a.
NCBI ID   WP_146329601.1    Uniprot ID   A0A5B8N693
Organism   Noviherbaspirillum sp. UKPF54     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1553601..1564971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAY22_RS07285 (FAY22_07285) rimI 1553834..1554325 (+) 492 WP_246860691.1 ribosomal protein S18-alanine N-acetyltransferase -
  FAY22_RS07290 (FAY22_07290) - 1554322..1555155 (+) 834 WP_146329597.1 uracil-DNA glycosylase family protein -
  FAY22_RS07295 (FAY22_07295) - 1555139..1556104 (+) 966 WP_146329598.1 DUF1853 family protein -
  FAY22_RS07300 (FAY22_07300) lplT 1556122..1557372 (-) 1251 WP_146329599.1 lysophospholipid transporter LplT -
  FAY22_RS07305 (FAY22_07305) alr 1557514..1558590 (+) 1077 WP_146329600.1 alanine racemase -
  FAY22_RS07310 (FAY22_07310) radA 1558601..1559971 (+) 1371 WP_146329601.1 DNA repair protein RadA Machinery gene
  FAY22_RS07315 (FAY22_07315) hpnE 1559977..1561317 (-) 1341 WP_146329602.1 hydroxysqualene dehydroxylase HpnE -
  FAY22_RS07320 (FAY22_07320) hpnD 1561304..1562137 (-) 834 WP_146329603.1 presqualene diphosphate synthase HpnD -
  FAY22_RS07325 (FAY22_07325) hpnC 1562237..1563046 (-) 810 WP_146329604.1 squalene synthase HpnC -
  FAY22_RS07330 (FAY22_07330) - 1563203..1564402 (+) 1200 WP_246860692.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48694.15 Da        Isoelectric Point: 7.7218

>NTDB_id=362391 FAY22_RS07310 WP_146329601.1 1558601..1559971(+) (radA) [Noviherbaspirillum sp. UKPF54]
MAKQKTNYTCTECGGVSSKWAGQCPSCGQWNTLVETVVEAAGNRFSGQYRGLAQTSPVLSLADIEAIDVPRFGTGIEEFD
RVLGGGLVAGGVVLIGGDPGIGKSTLLLQALANMSRLKKVLYVSGEESGAQIALRARRLAIDAKELQLQAEIQLEKILNT
LAEHKPQVAVIDSIQTIYSDALTSAPGSVAQVRECAAQLTRVAKQSGITIIMVGHVTKEGALAGPRVLEHIVDTVLYFEG
DTHSSFRLVRAFKNRFGAVNELGVFAMTEKGLKGVSNPSALFLSQHDSQVPGSCVMVTQEGTRPLLVEIQALVDTSHVPN
ARRLSVGLEQNRLAMLLAVLHRHAAIAAFDQDVFINAVGGVKITEPAADLAVLLAIHSSMRNKPLPRGLVVFGEVGLAGE
IRPAPRGQERLREAAKLGFSLALIPKANAPKQQIEGLNIIGVERIDEAMNRIRDAE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=362391 FAY22_RS07310 WP_146329601.1 1558601..1559971(+) (radA) [Noviherbaspirillum sp. UKPF54]
ATGGCAAAGCAAAAAACCAACTACACCTGCACCGAATGCGGCGGCGTCTCCAGTAAATGGGCCGGGCAGTGCCCCTCGTG
CGGGCAGTGGAACACGCTCGTCGAAACCGTGGTGGAAGCCGCCGGCAACCGCTTTTCGGGCCAGTATCGCGGCCTCGCGC
AAACCTCGCCCGTGCTCAGCCTGGCCGACATCGAAGCCATCGACGTGCCGCGCTTCGGCACAGGGATCGAGGAATTTGAC
CGCGTGCTGGGCGGCGGTCTGGTGGCGGGCGGCGTGGTGCTGATCGGAGGTGATCCAGGCATCGGCAAATCGACCCTGCT
GCTGCAGGCGCTGGCCAACATGTCGCGCCTGAAGAAGGTGCTGTACGTCAGCGGCGAGGAATCCGGCGCCCAGATCGCGC
TGCGCGCCCGGCGCCTTGCGATCGATGCGAAAGAATTGCAGCTGCAGGCCGAGATCCAGCTGGAAAAAATCCTCAATACG
CTGGCCGAACACAAGCCGCAGGTAGCGGTGATCGACTCGATCCAGACCATTTATTCCGATGCGCTGACGTCGGCGCCCGG
CTCGGTGGCGCAGGTGCGCGAATGCGCGGCGCAGCTCACGCGCGTCGCCAAGCAGTCGGGCATCACGATCATCATGGTCG
GCCATGTCACCAAGGAAGGTGCCCTGGCCGGGCCGCGCGTGCTGGAGCACATCGTCGACACCGTGCTGTATTTCGAAGGC
GACACGCATTCCAGTTTCCGCCTGGTGCGGGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCCAT
GACCGAGAAGGGGCTCAAGGGCGTCTCCAACCCTTCCGCACTGTTCCTGTCGCAGCATGACAGCCAGGTACCGGGCTCCT
GCGTGATGGTGACTCAGGAAGGTACTCGGCCCTTGCTGGTCGAGATCCAGGCGCTGGTCGATACCTCGCACGTGCCGAAT
GCGCGCCGGCTGTCGGTCGGACTGGAGCAGAACCGGCTGGCCATGCTGCTGGCAGTGCTGCACCGCCACGCCGCCATCGC
CGCATTCGACCAGGACGTCTTCATCAATGCCGTCGGCGGCGTGAAGATCACGGAGCCGGCCGCCGACCTGGCGGTGCTGC
TGGCGATTCATTCGTCGATGCGCAACAAGCCCTTGCCGCGCGGGCTGGTAGTGTTCGGCGAGGTCGGACTGGCCGGTGAA
ATCCGCCCCGCGCCGCGCGGGCAGGAGCGCTTGCGCGAAGCGGCGAAGCTGGGCTTTTCGCTGGCGCTGATCCCGAAAGC
CAATGCGCCCAAGCAGCAGATCGAAGGCTTGAACATCATCGGCGTCGAGCGCATCGACGAGGCGATGAACCGGATTCGCG
ACGCCGAGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(73-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(359-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8N693

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.455

100

0.498
  radA Streptococcus mitis NCTC 12261

47.807

100

0.478
  radA Streptococcus mitis SK321

47.807

100

0.478
  radA Streptococcus pneumoniae Rx1

47.702

100

0.478
  radA Streptococcus pneumoniae D39

47.702

100

0.478
  radA Streptococcus pneumoniae R6

47.702

100

0.478
  radA Streptococcus pneumoniae TIGR4

47.702

100

0.478

Multiple sequence alignment