Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FDQ49_RS06995 Genome accession   NZ_CP040105
Coordinates   1493503..1494279 (-) Length   258 a.a.
NCBI ID   WP_306302597.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis M2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1488503..1499279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDQ49_RS06975 (FDQ49_06975) - 1489568..1490266 (-) 699 WP_002053293.1 tetratricopeptide repeat protein -
  FDQ49_RS06980 (FDQ49_06980) hisS 1490295..1491587 (-) 1293 WP_002053284.1 histidine--tRNA ligase -
  FDQ49_RS06985 (FDQ49_06985) ispG 1491584..1492699 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FDQ49_RS06990 (FDQ49_06990) - 1492721..1493512 (-) 792 WP_002053491.1 RodZ domain-containing protein -
  FDQ49_RS06995 (FDQ49_06995) pilF 1493503..1494279 (-) 777 WP_306302597.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FDQ49_RS07000 (FDQ49_07000) rlmN 1494319..1495551 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FDQ49_RS07005 (FDQ49_07005) ndk 1495674..1496105 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  FDQ49_RS07010 (FDQ49_07010) iscX 1496219..1496419 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  FDQ49_RS07015 (FDQ49_07015) - 1496519..1497070 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  FDQ49_RS07020 (FDQ49_07020) - 1497095..1498393 (-) 1299 WP_080660936.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 258 a.a.        Molecular weight: 29198.28 Da        Isoelectric Point: 8.7393

>NTDB_id=362217 FDQ49_RS06995 WP_306302597.1 1493503..1494279(-) (pilF) [Acinetobacter nosocomialis M2]
MLCMGVAVAFLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGILLQQEGSKP
NLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDIASAEKTFK
QALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQLRALFPES
PEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 777 bp        

>NTDB_id=362217 FDQ49_RS06995 WP_306302597.1 1493503..1494279(-) (pilF) [Acinetobacter nosocomialis M2]
ATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCCAGTGGTTGTCAGTCTTCGCCAACGCTTAAAAAGGACCCGGAAAA
AGCAGTTAAGGTGCGTACGCAGCTTGCTGCTGAATACATTCGTTCTGGTGACTTGGACTCTGCAAAGCGTTCCCTTGACC
AAGCTTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTATTGCAACAAGAAGGCAGTAAACCC
AATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAATGCTCAGGCGCGTAATAATTATGG
TACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTGCTGGTGCAACATTAGGTTATGATC
AACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATTGCGAGTGCTGAAAAAACATTCAAA
CAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAATTTTTTATTTGCAGCAGCAGATTCC
GGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGGGTGCTCGCGCACTTTGGATTGGTT
TAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAGTTACGTGCTTTATTCCCGGAAAGT
CCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(141-198)

Tetratricopeptide repeat (TPR_16)

(81-126)

Tetratricopeptide repeat (TPR_8)

(31-60)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.45

100

0.985
  pilF Acinetobacter baylyi ADP1

75.391

99.225

0.748

Multiple sequence alignment