Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FDM99_RS05270 Genome accession   NZ_CP040080
Coordinates   1080916..1081716 (+) Length   266 a.a.
NCBI ID   WP_212771375.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain SP304     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075916..1086716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDM99_RS05245 (FDM99_05245) - 1076826..1078124 (+) 1299 WP_085940557.1 glycosyltransferase family 4 protein -
  FDM99_RS05250 (FDM99_05250) - 1078149..1078700 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  FDM99_RS05255 (FDM99_05255) iscX 1078800..1079000 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  FDM99_RS05260 (FDM99_05260) ndk 1079114..1079545 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  FDM99_RS05265 (FDM99_05265) rlmN 1079668..1080900 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  FDM99_RS05270 (FDM99_05270) pilF 1080916..1081716 (+) 801 WP_212771375.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FDM99_RS05275 (FDM99_05275) - 1081707..1082498 (+) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  FDM99_RS05280 (FDM99_05280) ispG 1082520..1083635 (+) 1116 WP_032025406.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FDM99_RS05285 (FDM99_05285) hisS 1083632..1084924 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  FDM99_RS05290 (FDM99_05290) - 1084953..1085651 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30033.31 Da        Isoelectric Point: 9.0211

>NTDB_id=361923 FDM99_RS05270 WP_212771375.1 1080916..1081716(+) (pilF) [Acinetobacter baumannii strain SP304]
MSTPKLKIMLCMGGAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=361923 FDM99_RS05270 WP_212771375.1 1080916..1081716(+) (pilF) [Acinetobacter baumannii strain SP304]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGGTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACATAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment