Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   E8M85_RS06180 Genome accession   NZ_CP040016
Coordinates   1156325..1157284 (+) Length   319 a.a.
NCBI ID   WP_002875425.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni strain 9090     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1136618..1166621 1156325..1157284 within 0


Gene organization within MGE regions


Location: 1136618..1166621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M85_RS06085 (E8M85_06170) pglI 1136618..1137547 (-) 930 WP_002890470.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  E8M85_RS06090 (E8M85_06175) pglH 1137540..1138619 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  E8M85_RS06095 (E8M85_06180) pglK 1138616..1140310 (-) 1695 WP_002890469.1 ABC-type lipopolysaccharide transporter PglK -
  E8M85_RS06100 (E8M85_06185) galE 1140304..1141290 (-) 987 WP_002853529.1 UDP-glucose 4-epimerase GalE -
  E8M85_RS06105 (E8M85_06190) - 1141348..1142142 (-) 795 WP_002853812.1 3'-5' exonuclease -
  E8M85_RS06110 (E8M85_06195) waaC 1142208..1143236 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  E8M85_RS06115 (E8M85_06200) htrB 1143226..1144113 (+) 888 WP_002890465.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  E8M85_RS06120 (E8M85_06205) - 1144110..1145657 (+) 1548 WP_002858148.1 glycosyltransferase -
  E8M85_RS06125 (E8M85_06210) - 1145661..1146833 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  E8M85_RS06130 (E8M85_06215) - 1146817..1147812 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  E8M85_RS06135 (E8M85_06220) - 1147870..1149039 (+) 1170 WP_002858320.1 glycosyltransferase family 2 protein -
  E8M85_RS06140 (E8M85_06225) wlaN 1149028..1149940 (-) 913 Protein_1193 beta-1,3 galactosyltransferase -
  E8M85_RS06145 (E8M85_06230) cstIII 1149995..1150879 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  E8M85_RS06150 (E8M85_06235) neuB1 1150860..1151891 (+) 1032 WP_002858213.1 sialic acid synthase -
  E8M85_RS06155 (E8M85_06240) neuC 1151888..1153003 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  E8M85_RS09285 - 1153000..1153914 (+) 915 Protein_1197 beta-1,4-N-acetylgalactosaminyltransferase -
  E8M85_RS09290 - 1153948..1154610 (+) 663 WP_002881429.1 N-acylneuraminate cytidylyltransferase -
  E8M85_RS06170 (E8M85_06250) - 1154605..1155458 (-) 854 Protein_1199 glycosyltransferase -
  E8M85_RS06175 (E8M85_06255) waaV 1155442..1156266 (-) 825 WP_002858096.1 glycosyltransferase family 2 protein -
  E8M85_RS06180 (E8M85_06260) waaF 1156325..1157284 (+) 960 WP_002875425.1 lipopolysaccharide heptosyltransferase II Regulator
  E8M85_RS06185 (E8M85_06265) gmhA 1157265..1157825 (-) 561 WP_002858021.1 D-sedoheptulose 7-phosphate isomerase -
  E8M85_RS06190 (E8M85_06270) rfaE1 1157822..1159207 (-) 1386 WP_002890180.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  E8M85_RS06195 (E8M85_06275) rfaD 1159200..1160153 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  E8M85_RS06200 (E8M85_06280) gmhB 1160154..1160714 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  E8M85_RS06205 (E8M85_06285) - 1160796..1161098 (+) 303 WP_002852762.1 cytochrome c -
  E8M85_RS06210 (E8M85_06290) ccoS 1161121..1161327 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  E8M85_RS06215 (E8M85_06295) - 1161324..1163681 (-) 2358 WP_072224466.1 heavy metal translocating P-type ATPase -
  E8M85_RS06220 (E8M85_06300) rho 1163790..1165088 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  E8M85_RS06225 (E8M85_06305) dnaX 1165092..1166621 (+) 1530 WP_002875420.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=361612 E8M85_RS06180 WP_002875425.1 1156325..1157284(+) (waaF) [Campylobacter jejuni subsp. jejuni strain 9090]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=361612 E8M85_RS06180 WP_002875425.1 1156325..1157284(+) (waaF) [Campylobacter jejuni subsp. jejuni strain 9090]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

100

100

1

Multiple sequence alignment