Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FCJ76_RS12940 Genome accession   NZ_CP039935
Coordinates   2501607..2502017 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain H19     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2496607..2507017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCJ76_RS12910 - 2496694..2496834 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  FCJ76_RS12915 yqeF 2497140..2497871 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  FCJ76_RS12920 cwlH 2498123..2498875 (-) 753 WP_101169548.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  FCJ76_RS12925 yqeD 2499062..2499688 (+) 627 WP_033884461.1 TVP38/TMEM64 family protein -
  FCJ76_RS12930 gnd 2499707..2500600 (-) 894 WP_047182906.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  FCJ76_RS12935 yqeB 2500852..2501574 (+) 723 WP_134983516.1 DUF308 domain-containing protein -
  FCJ76_RS12940 nucA/comI 2501607..2502017 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  FCJ76_RS12945 sigK 2502213..2502941 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  FCJ76_RS21400 - 2502941..2503056 (+) 116 Protein_2485 hypothetical protein -
  FCJ76_RS12950 - 2503043..2503219 (-) 177 Protein_2486 recombinase family protein -
  FCJ76_RS21405 - 2503177..2503355 (-) 179 Protein_2487 hypothetical protein -
  FCJ76_RS12960 psiE 2503919..2504335 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  FCJ76_RS12965 yrkQ 2504381..2505679 (-) 1299 WP_134983213.1 two-component system sensor histidine kinase YrkQ -
  FCJ76_RS12970 yrkP 2505666..2506361 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=361145 FCJ76_RS12940 WP_009967785.1 2501607..2502017(-) (nucA/comI) [Bacillus subtilis strain H19]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=361145 FCJ76_RS12940 WP_009967785.1 2501607..2502017(-) (nucA/comI) [Bacillus subtilis strain H19]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment