Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GUU86_RS19160 Genome accession   NZ_CP047665
Coordinates   4009641..4010081 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain LD26-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4004641..4015081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUU86_RS19140 (GUU86_19140) coaE 4005131..4005751 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  GUU86_RS25055 - 4005776..4005820 (+) 45 WP_120795372.1 protein YacM -
  GUU86_RS19145 (GUU86_19145) guaC 4005976..4007019 (+) 1044 WP_001217338.1 GMP reductase -
  GUU86_RS19150 (GUU86_19150) hofC 4007054..4008256 (-) 1203 WP_000157234.1 protein transport protein HofC -
  GUU86_RS19155 (GUU86_19155) pilB 4008246..4009631 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  GUU86_RS19160 (GUU86_19160) pilA 4009641..4010081 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  GUU86_RS19165 (GUU86_19165) nadC 4010284..4011177 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GUU86_RS19170 (GUU86_19170) ampD 4011265..4011816 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GUU86_RS19175 (GUU86_19175) ampE 4011813..4012667 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  GUU86_RS19180 (GUU86_19180) aroP 4012710..4014083 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=360808 GUU86_RS19160 WP_000360895.1 4009641..4010081(-) (pilA) [Escherichia coli strain LD26-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=360808 GUU86_RS19160 WP_000360895.1 4009641..4010081(-) (pilA) [Escherichia coli strain LD26-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment