Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GUU84_RS03220 Genome accession   NZ_CP047662
Coordinates   704483..704923 (-) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain LD93-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699483..709923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUU84_RS03200 (GUU84_03200) coaE 699973..700593 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  GUU84_RS23455 - 700618..700662 (+) 45 WP_120795372.1 protein YacM -
  GUU84_RS03205 (GUU84_03205) guaC 700818..701861 (+) 1044 WP_001217338.1 GMP reductase -
  GUU84_RS03210 (GUU84_03210) hofC 701896..703098 (-) 1203 WP_000157234.1 protein transport protein HofC -
  GUU84_RS03215 (GUU84_03215) pilB 703088..704473 (-) 1386 WP_040234494.1 type II secretion system protein GspE Machinery gene
  GUU84_RS03220 (GUU84_03220) pilA 704483..704923 (-) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  GUU84_RS03225 (GUU84_03225) nadC 705126..706019 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GUU84_RS03230 (GUU84_03230) ampD 706107..706658 (+) 552 WP_000923720.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GUU84_RS03235 (GUU84_03235) ampE 706655..707509 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  GUU84_RS03240 (GUU84_03240) aroP 707552..708925 (-) 1374 WP_201762819.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=360668 GUU84_RS03220 WP_000360901.1 704483..704923(-) (pilA) [Escherichia coli strain LD93-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=360668 GUU84_RS03220 WP_000360901.1 704483..704923(-) (pilA) [Escherichia coli strain LD93-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363