Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   FAY30_RS07315 Genome accession   NZ_CP039727
Coordinates   1536818..1540597 (+) Length   1259 a.a.
NCBI ID   WP_149869250.1    Uniprot ID   -
Organism   Bacillus sp. S3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1531818..1545597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAY30_RS07300 (FAY30_07300) - 1531962..1532627 (+) 666 WP_149869247.1 TVP38/TMEM64 family protein -
  FAY30_RS07305 (FAY30_07305) lepB 1532617..1533171 (+) 555 WP_149869248.1 signal peptidase I -
  FAY30_RS07310 (FAY30_07310) addB 1533328..1536825 (+) 3498 WP_149869249.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  FAY30_RS07315 (FAY30_07315) addA 1536818..1540597 (+) 3780 WP_149869250.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  FAY30_RS07320 (FAY30_07320) - 1540640..1540858 (-) 219 WP_149869251.1 spore germination protein -
  FAY30_RS07325 (FAY30_07325) - 1540921..1541307 (-) 387 WP_149869252.1 spore germination protein GerPE -
  FAY30_RS07330 (FAY30_07330) - 1541288..1541494 (-) 207 WP_149869253.1 spore gernimation protein GerPD -
  FAY30_RS07335 (FAY30_07335) gerPC 1541491..1542108 (-) 618 WP_149869254.1 spore germination protein GerPC -
  FAY30_RS07340 (FAY30_07340) - 1542223..1542459 (-) 237 WP_149869255.1 spore germination protein GerPB -
  FAY30_RS07345 (FAY30_07345) - 1542476..1542697 (-) 222 WP_149869256.1 spore germination protein -
  FAY30_RS07350 (FAY30_07350) - 1543180..1543512 (+) 333 WP_223820985.1 DUF192 domain-containing protein -
  FAY30_RS07355 (FAY30_07355) - 1543529..1543708 (+) 180 WP_149869258.1 Flp family type IVb pilin -
  FAY30_RS07360 (FAY30_07360) - 1543823..1544332 (+) 510 WP_149869259.1 prepilin peptidase -
  FAY30_RS07365 (FAY30_07365) - 1544329..1544712 (+) 384 WP_149869260.1 TadE/TadG family type IV pilus assembly protein -

Sequence

Protein


Download         Length: 1259 a.a.        Molecular weight: 144912.63 Da        Isoelectric Point: 5.2234

>NTDB_id=360447 FAY30_RS07315 WP_149869250.1 1536818..1540597(+) (addA) [Bacillus sp. S3]
MSNFMIPSKPEGVTWTDDQWKAIVARNKDILVAAAAGSGKTAVLVERIIQKILSMDDPLNVDELLVVTFTNASAAEMRHR
IGEALEKAIDLDPGSRHLRKQLTLLNKASISTLHSFCMEVIRKYYYLIDIDPGFRIADETEAQLIRDEVMDELFEEEYGK
KDNQAFFTLVDSFTSDRSDAGLMEIVRSIYDFARSNPQPEQYLQSIVSMYDVADGDNIENLPFTEALLFDIKLQLEAAKE
MIKRGLEVTKLPNGPAPRAENFLDDLLVIETLLSAQKDSWATLHQAMQAWSFSRAKQVKGDHYDKDLTDKAQKLREKAKK
KIQDVKEELFSRKPESFLRDMREMLPLVETLAYLVREFSNRFEKIKKEKSLVDYADLEHYCLAILTEAISAEGEFLPSEA
ALAYRGHFKEVYCDEYQDTNMVQETILKLVTKDGEASGNLFMVGDVKQSIYRFRLAEPNLFLSKYNRFRVDGIDSGLRID
LARNFRSRKEVLDATNYLFKQIMGIKVGEIEYDEAAELIKGAPYPAEDPYPVEVLLIDQNAEEAEAAGDKEAEGEIEAAE
FDAAELEQSQLEARVIAGKIKELVAAGAPVYNTKTKSQSAIMYRDIVILLRSMTWAPQIIEEFKQQGIPIYANLSTGYFE
ATEVSIMMSLLRVIDNPYQDIPLASVLRSPIVGLNEEDLSKIRIHRKHGSFWEAVSSFCLSKTAEDTEPFYAKVRSFFDW
LTSWRSLARQGALSELIWQLYRDTGFYDFVGGLPGGKQRQANLRALYDRARAYEQTSFRGLFRFLRFIERMMDRGDDLGA
ARALSEQEDVVRIMTIHSSKGLEFPVVFMAGMARNFNMMDIRKSYMLDKEFGFAAKYVNAEKRISYPSLPQIAFKRKKRM
EMLAEEMRVLYVAMTRAKEKLFMTAALKDAAKAIDKWNDVSSHTEWLLEDFERASAMSYIDWIGPALIRHQNCDELRNTS
EKGKRVPVEITEHPSSWKIKVKSAEEIKKQETKLNMQEDYFLERVQKFEQVPAESSFAMEIKDRLTWEYSFPDATVHRSK
QSVSEIKRFAEMTDEHSGTELVRRFKKSIIKRPKFMQEKQLSPAERGTAMHMVMQHVDLTIPVSEESIRRQLDWMVDNEL
LTPEQSAVIDSELIVQFFNSNLGRRYFHAKAIHREIPFTLSLPASEVYPNWKEADEFVFVQGIIDCILEDDHGLVLIDYK
TDGITGRYRGGFHQAAPILEERYRVQINLYTKAIEQIWKRDVTERYLFFFDGAHILKVE

Nucleotide


Download         Length: 3780 bp        

>NTDB_id=360447 FAY30_RS07315 WP_149869250.1 1536818..1540597(+) (addA) [Bacillus sp. S3]
ATGAGTAATTTCATGATCCCTTCGAAGCCAGAGGGTGTGACCTGGACAGATGATCAGTGGAAAGCAATAGTGGCAAGGAA
TAAAGATATCCTTGTAGCGGCAGCGGCCGGTTCAGGGAAAACTGCTGTCCTTGTGGAAAGAATCATCCAAAAAATTCTTT
CCATGGATGATCCGCTTAATGTTGATGAATTGTTGGTCGTGACGTTTACCAATGCTTCCGCAGCAGAAATGCGGCATCGA
ATTGGGGAAGCATTGGAAAAAGCAATTGATCTGGATCCGGGTTCAAGACATTTACGAAAGCAGCTCACCTTATTAAATAA
GGCATCGATTTCAACTCTGCATTCCTTTTGTATGGAGGTCATCCGTAAGTATTATTATTTAATTGATATTGACCCGGGTT
TTCGGATTGCCGATGAAACGGAGGCACAATTAATAAGAGATGAAGTAATGGATGAATTGTTTGAAGAAGAGTATGGAAAA
AAGGATAATCAGGCCTTTTTTACTTTAGTCGATTCGTTCACGAGTGATCGAAGTGATGCTGGATTAATGGAAATTGTGCG
CTCAATCTACGATTTTGCCCGTTCAAACCCCCAGCCGGAGCAGTATTTACAATCTATTGTTTCCATGTATGACGTGGCAG
ATGGAGACAATATAGAAAATCTCCCGTTTACAGAGGCACTTCTTTTCGATATTAAATTGCAATTAGAAGCGGCAAAGGAA
ATGATCAAGCGAGGGCTTGAAGTAACCAAACTGCCAAATGGCCCGGCGCCGAGAGCCGAAAACTTCTTAGATGATTTGCT
AGTCATTGAAACACTTTTATCAGCGCAAAAAGATTCATGGGCGACACTACATCAAGCGATGCAAGCATGGTCATTTTCAC
GGGCAAAACAGGTTAAGGGAGACCACTATGATAAGGACCTGACTGATAAGGCCCAGAAGCTCCGGGAAAAGGCGAAGAAA
AAAATCCAAGATGTAAAAGAAGAGCTCTTTTCCAGGAAACCGGAAAGCTTTTTACGTGATATGAGAGAGATGCTGCCACT
TGTCGAAACGCTTGCCTATCTGGTAAGAGAATTTTCAAACCGATTTGAAAAAATAAAAAAAGAAAAAAGTCTTGTTGATT
ATGCCGATTTAGAGCACTACTGTCTCGCAATATTGACAGAAGCCATTTCAGCTGAAGGTGAATTTCTCCCATCAGAAGCA
GCACTTGCTTACCGCGGGCATTTTAAAGAAGTTTATTGTGATGAATATCAAGACACAAATATGGTTCAGGAAACGATCTT
AAAACTTGTAACAAAAGATGGGGAAGCAAGTGGTAATTTATTCATGGTCGGCGATGTCAAGCAGTCGATTTACCGCTTTC
GACTAGCAGAGCCAAATCTCTTCTTAAGTAAATATAACCGCTTTAGGGTGGATGGCATTGATTCTGGCTTAAGAATCGAT
TTGGCACGGAATTTCAGAAGTAGGAAAGAAGTACTTGATGCCACCAATTATTTATTCAAACAAATCATGGGGATCAAAGT
TGGAGAAATAGAATATGATGAGGCGGCGGAATTGATAAAGGGTGCACCATACCCAGCAGAAGATCCATATCCGGTAGAAG
TGCTGCTAATTGATCAAAATGCGGAAGAGGCAGAAGCGGCCGGAGATAAAGAAGCGGAAGGCGAAATAGAAGCGGCTGAA
TTTGATGCAGCAGAGCTGGAACAATCGCAGCTTGAAGCAAGAGTCATAGCTGGAAAGATTAAAGAATTAGTTGCAGCAGG
TGCACCTGTATATAATACAAAAACAAAATCGCAAAGCGCTATTATGTATCGGGATATCGTGATTCTCCTCCGCTCGATGA
CCTGGGCACCGCAAATTATTGAAGAGTTTAAGCAACAAGGAATTCCGATTTATGCGAATTTATCCACTGGATATTTTGAA
GCGACAGAGGTATCAATTATGATGTCACTGCTCAGGGTGATTGATAATCCATACCAGGATATCCCTTTAGCTTCGGTGTT
ACGGTCACCCATCGTCGGATTAAATGAAGAGGATTTATCTAAAATTCGCATTCACAGGAAGCATGGCTCGTTTTGGGAGG
CCGTTTCTTCCTTTTGCTTGAGTAAAACAGCCGAAGATACAGAGCCTTTTTATGCTAAAGTCCGAAGTTTTTTTGATTGG
TTAACAAGCTGGCGCTCGCTGGCAAGACAAGGAGCGCTTTCAGAATTAATCTGGCAGTTATACAGGGATACTGGGTTTTA
TGATTTTGTTGGCGGACTGCCTGGAGGCAAACAGCGTCAGGCTAACTTAAGAGCTTTATATGACAGGGCAAGGGCCTATG
AACAGACCTCTTTCCGCGGGTTATTTCGCTTTTTGCGATTTATCGAGCGCATGATGGATCGAGGAGATGACCTCGGGGCC
GCAAGAGCGCTGAGTGAGCAGGAGGATGTTGTCCGGATTATGACGATTCATAGCAGTAAAGGGCTTGAATTTCCAGTTGT
ATTTATGGCGGGGATGGCACGGAATTTTAATATGATGGATATCCGAAAGTCCTACATGCTAGATAAAGAATTTGGATTTG
CGGCTAAATATGTAAATGCAGAGAAACGTATTTCCTATCCATCCCTCCCGCAAATTGCTTTTAAACGGAAAAAAAGAATG
GAAATGCTAGCAGAGGAGATGCGTGTCTTATACGTGGCGATGACGAGGGCGAAAGAAAAGCTCTTTATGACAGCAGCTTT
AAAGGATGCGGCGAAAGCAATCGACAAATGGAATGATGTTTCCTCACATACTGAATGGCTGTTAGAGGATTTTGAGCGGG
CATCGGCCATGAGCTATATCGACTGGATTGGACCGGCATTGATCCGTCATCAAAACTGCGATGAATTAAGGAATACAAGT
GAAAAAGGTAAACGTGTACCGGTGGAAATCACTGAGCATCCGTCTTCTTGGAAAATTAAAGTGAAAAGTGCAGAAGAAAT
TAAAAAACAGGAAACGAAACTTAACATGCAAGAGGATTATTTTCTGGAAAGAGTTCAAAAGTTTGAGCAAGTCCCGGCAG
AATCTTCATTTGCTATGGAAATAAAGGACCGGCTGACATGGGAATATTCCTTCCCCGATGCCACAGTCCATCGTTCAAAG
CAATCTGTTTCAGAAATTAAACGCTTCGCAGAAATGACGGATGAGCATAGCGGTACGGAATTAGTTCGAAGATTTAAAAA
GTCAATTATAAAACGGCCAAAGTTTATGCAAGAAAAACAGTTGAGCCCTGCCGAACGGGGGACGGCGATGCATATGGTGA
TGCAGCATGTGGATTTGACAATCCCTGTTAGTGAAGAATCCATTCGGAGGCAATTAGATTGGATGGTTGACAATGAATTG
TTAACTCCGGAGCAGTCCGCGGTTATTGACAGTGAATTAATAGTGCAATTTTTCAACTCAAATCTAGGCAGAAGATATTT
TCATGCCAAAGCCATTCACAGGGAAATTCCATTCACTCTTTCATTGCCTGCTAGTGAGGTTTACCCAAACTGGAAAGAAG
CAGATGAATTTGTATTTGTCCAAGGAATCATTGATTGTATTCTGGAGGATGATCATGGGCTTGTCCTGATTGACTATAAA
ACAGATGGAATAACAGGAAGGTATAGGGGAGGCTTTCATCAGGCCGCACCAATCCTTGAAGAAAGATACAGGGTGCAGAT
TAACTTATATACGAAGGCGATCGAACAAATCTGGAAAAGAGATGTCACAGAAAGATATTTATTTTTCTTTGATGGAGCGC
ACATTTTAAAAGTAGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1074-1246)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(15-468)

UvrD-like helicase C-terminal domain (UvrD_C)

(479-906)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

55.458

99.682

0.553

Multiple sequence alignment