Detailed information NTG0360417

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: FBF59_RS21740
• Genome accession: NZ_CP039721
• Coordinates: 4232606..4233955 (+)
• Length: 449 a.a.
• NCBI ID: WP_002083580.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain BT-59
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4227606..4238955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FBF59_RS21720 (FBF59_21720)	-	4228743..4229378 (-)	636	WP_000926669.1	YigZ family protein	-
FBF59_RS21725 (FBF59_21725)	-	4229615..4230457 (+)	843	WP_000684726.1	DegV family protein	-
FBF59_RS21730 (FBF59_21730)	-	4230630..4230944 (+)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
FBF59_RS21735 (FBF59_21735)	-	4231093..4232478 (+)	1386	WP_000499483.1	NlpC/P60 family protein	-
FBF59_RS21740 (FBF59_21740)	comFA	4232606..4233955 (+)	1350	WP_002083580.1	ATP-dependent helicase ComFA	Machinery gene
FBF59_RS21745 (FBF59_21745)	-	4233955..4234659 (+)	705	WP_003255239.1	ComF family protein	-
FBF59_RS21750 (FBF59_21750)	cspC	4234786..4234983 (+)	198	WP_001990088.1	cold shock protein CspC	-
FBF59_RS21755 (FBF59_21755)	raiA	4235305..4235847 (+)	543	WP_000671187.1	ribosome-associated translation inhibitor RaiA	-
FBF59_RS21760 (FBF59_21760)	secA	4236120..4238627 (+)	2508	WP_000579374.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51166.05 Da
• Isoelectric Point: 10.0230

>NTDB_id=360417 FBF59_RS21740 WP_002083580.1 4232606..4233955(+) (comFA) [Bacillus thuringiensis strain BT-59]
MLAGKQLLLDELSSDLQRELNDLKRKGEVICVQGVKKKNSKYMCQRCGNVDQRLFASFLCRRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKKLNLLQWNGKLSTGQNLAAQGVVEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKHPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=360417 FBF59_RS21740 WP_002083580.1 4232606..4233955(+) (comFA) [Bacillus thuringiensis strain BT-59]
ATGCTGGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAGGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCAGCGGC
TATTTGCGTCGTTTTTATGTAGAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAAATTAAACTTGTTACAGTGGAACGGGAAGTT
GTCTACTGGTCAGAATTTGGCGGCACAAGGTGTTGTAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGGGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAACATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAATCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTAGCGGTTTTAGGGGCTGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCCAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.131	98.441	0.503
comFA	Latilactobacillus sakei subsp. sakei 23K	41.709	88.641	0.37