Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GUK18_RS18960 Genome accession   NZ_CP047600
Coordinates   3957857..3958297 (-) Length   146 a.a.
NCBI ID   WP_024223365.1    Uniprot ID   -
Organism   Escherichia coli O84:H7 strain Trh52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952857..3963297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUK18_RS18935 (GUK18_18955) coaE 3953347..3953967 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  GUK18_RS18940 - 3953992..3954036 (+) 45 WP_120795372.1 protein YacM -
  GUK18_RS18945 (GUK18_18960) guaC 3954192..3955235 (+) 1044 WP_001217338.1 GMP reductase -
  GUK18_RS18950 (GUK18_18965) hofC 3955270..3956472 (-) 1203 WP_024223366.1 protein transport protein HofC -
  GUK18_RS18955 (GUK18_18970) pilB 3956462..3957847 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  GUK18_RS18960 (GUK18_18975) pilA 3957857..3958297 (-) 441 WP_024223365.1 prepilin peptidase-dependent pilin Machinery gene
  GUK18_RS18965 (GUK18_18980) nadC 3958501..3959394 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GUK18_RS18970 (GUK18_18985) ampD 3959482..3960033 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GUK18_RS18975 (GUK18_18990) ampE 3960030..3960884 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  GUK18_RS18980 (GUK18_18995) aroP 3960927..3962297 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15662.85 Da        Isoelectric Point: 4.5237

>NTDB_id=360376 GUK18_RS18960 WP_024223365.1 3957857..3958297(-) (pilA) [Escherichia coli O84:H7 strain Trh52]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVVKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=360376 GUK18_RS18960 WP_024223365.1 3957857..3958297(-) (pilA) [Escherichia coli O84:H7 strain Trh52]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGTAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363