Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GUK18_RS13105 Genome accession   NZ_CP047600
Coordinates   2696819..2698315 (-) Length   498 a.a.
NCBI ID   WP_001040641.1    Uniprot ID   -
Organism   Escherichia coli O84:H7 strain Trh52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2691819..2703315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUK18_RS13080 (GUK18_13110) panM 2692053..2692436 (+) 384 WP_024223023.1 aspartate 1-decarboxylase autocleavage activator PanM -
  GUK18_RS13085 (GUK18_13115) livJ 2692624..2693727 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GUK18_RS13090 (GUK18_13120) rpoH 2693998..2694852 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GUK18_RS13095 (GUK18_13125) ftsX 2695097..2696155 (-) 1059 WP_203164299.1 permease-like cell division protein FtsX -
  GUK18_RS13100 (GUK18_13130) ftsE 2696148..2696816 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GUK18_RS13105 (GUK18_13135) pilA 2696819..2698315 (-) 1497 WP_001040641.1 signal recognition particle-docking protein FtsY Machinery gene
  GUK18_RS13110 (GUK18_13140) rsmD 2698465..2699061 (+) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GUK18_RS13115 (GUK18_13145) yhhL 2699051..2699320 (+) 270 WP_001295207.1 DUF1145 family protein -
  GUK18_RS13120 (GUK18_13150) yhhM 2699323..2699682 (-) 360 WP_024223025.1 DUF2500 domain-containing protein -
  GUK18_RS13125 (GUK18_13155) yhhN 2699823..2700449 (+) 627 WP_000964718.1 lysoplasmalogenase -
  GUK18_RS13130 (GUK18_13160) zntA 2700523..2702721 (+) 2199 WP_024223026.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GUK18_RS13135 (GUK18_13165) tusA 2702990..2703235 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54614.37 Da        Isoelectric Point: 4.1733

>NTDB_id=360344 GUK18_RS13105 WP_001040641.1 2696819..2698315(-) (pilA) [Escherichia coli O84:H7 strain Trh52]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=360344 GUK18_RS13105 WP_001040641.1 2696819..2698315(-) (pilA) [Escherichia coli O84:H7 strain Trh52]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378