Detailed information NTG0360328

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: GUK18_RS09420
• Genome accession: NZ_CP047600
• Coordinates: 1937250..1938242 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli O84:H7 strain Trh52
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1932250..1943242

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GUK18_RS09400 (GUK18_09430)	ygbM	1932882..1933658 (+)	777	WP_001136928.1	HPr family phosphocarrier protein	-
GUK18_RS09405 (GUK18_09435)	ygbN	1933747..1935111 (+)	1365	WP_024223273.1	GntP family transporter	-
GUK18_RS09410 (GUK18_09440)	-	1935254..1936297 (-)	1044	WP_024235040.1	MBL fold metallo-hydrolase	-
GUK18_RS09415 (GUK18_09445)	-	1936521..1937072 (+)	552	WP_001179443.1	flavodoxin family protein	-
GUK18_RS09420 (GUK18_09450)	rpoS	1937250..1938242 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
GUK18_RS09425 (GUK18_09455)	nlpD	1938305..1939399 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
GUK18_RS09430 (GUK18_09460)	pcm	1939584..1940210 (-)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
GUK18_RS09435 (GUK18_09465)	surE	1940204..1940965 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
GUK18_RS09440 (GUK18_09470)	truD	1940946..1941995 (-)	1050	WP_000568928.1	tRNA pseudouridine(13) synthase TruD	-
GUK18_RS09445 (GUK18_09475)	ispF	1941992..1942471 (-)	480	WP_001219238.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
GUK18_RS09450 (GUK18_09480)	ispD	1942471..1943181 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=360328 GUK18_RS09420 WP_000081550.1 1937250..1938242(-) (rpoS) [Escherichia coli O84:H7 strain Trh52]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=360328 GUK18_RS09420 WP_000081550.1 1937250..1938242(-) (rpoS) [Escherichia coli O84:H7 strain Trh52]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715