Detailed information NTG0359824

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: GTW28_RS01650
• Genome accession: NZ_CP047485
• Coordinates: 294069..295190 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain BJQ0005
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 289069..300190

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTW28_RS01625 (GTW28_01625)	ycdA	289074..290138 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
GTW28_RS01630 (GTW28_01630)	-	290161..290292 (-)	132	WP_003246334.1	hypothetical protein	-
GTW28_RS01635 (GTW28_01635)	ycdB	290466..291887 (+)	1422	WP_046380765.1	YcdB/YcdC domain-containing protein	-
GTW28_RS01640 (GTW28_01640)	ycdC	292074..293435 (+)	1362	WP_159403949.1	YcdB/YcdC domain-containing protein	-
GTW28_RS01645 (GTW28_01645)	cwlK	293443..293946 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
GTW28_RS01650 (GTW28_01650)	rapF	294069..295190 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
GTW28_RS01655 (GTW28_01655)	ycdF	295297..296073 (+)	777	WP_019712418.1	SDR family oxidoreductase	-
GTW28_RS01660 (GTW28_01660)	ycdG	296098..297783 (+)	1686	WP_159403950.1	alpha-glucosidase	-
GTW28_RS01665 (GTW28_01665)	znuA	297971..298930 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
GTW28_RS01670 (GTW28_01670)	znuC	298986..299681 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=359824 GTW28_RS01650 WP_003246478.1 294069..295190(+) (rapF) [Bacillus subtilis strain BJQ0005]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=359824 GTW28_RS01650 WP_003246478.1 294069..295190(+) (rapF) [Bacillus subtilis strain BJQ0005]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGACACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405