Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GSY53_RS20840 Genome accession   NZ_CP047325
Coordinates   4058794..4059204 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain GOT9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4053794..4064204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSY53_RS20810 (GSY53_20810) - 4053881..4054021 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GSY53_RS20815 (GSY53_20815) yqeF 4054327..4055058 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  GSY53_RS20820 (GSY53_20820) cwlH 4055310..4056062 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GSY53_RS20825 (GSY53_20825) yqeD 4056249..4056875 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  GSY53_RS20830 (GSY53_20830) gnd 4056894..4057787 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GSY53_RS20835 (GSY53_20835) yqeB 4058039..4058761 (+) 723 WP_010886572.1 hypothetical protein -
  GSY53_RS20840 (GSY53_20840) nucA/comI 4058794..4059204 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GSY53_RS20845 (GSY53_20845) - 4059400..4059747 (+) 348 Protein_4065 sigma-70 family RNA polymerase sigma factor -
  GSY53_RS20850 (GSY53_20850) spoIVCA 4059778..4061238 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  GSY53_RS21485 - 4061196..4061374 (-) 179 Protein_4067 hypothetical protein -
  GSY53_RS20855 (GSY53_20855) arsC 4061729..4062148 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  GSY53_RS20860 (GSY53_20860) acr3 4062160..4063200 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  GSY53_RS20865 (GSY53_20865) arsK 4063223..4063663 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  GSY53_RS20870 (GSY53_20870) arsR 4063724..4064041 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=359405 GSY53_RS20840 WP_009967785.1 4058794..4059204(-) (nucA/comI) [Bacillus subtilis strain GOT9]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=359405 GSY53_RS20840 WP_009967785.1 4058794..4059204(-) (nucA/comI) [Bacillus subtilis strain GOT9]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment