Detailed information NTG0035932

Bioinformatically predicted

Overview

• Name: sepM
• Type: Regulator
• Locus tag: LLKF_RS12075
• Genome accession: NC_013656
• Coordinates: 2463302..2464330 (-)
• Length: 342 a.a.
• NCBI ID: WP_003132657.1
• Uniprot ID: S6FVM6
• Organism: Lactococcus lactis subsp. lactis KF147
• Function: processing of CSP (predicted from homology) Competence regulation

Genomic Context

Location: 2458302..2469330

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLKF_RS12060 (LLKF_2403)	-	2459376..2460233 (-)	858	WP_012898643.1	Rgg/GadR/MutR family transcriptional regulator	-
LLKF_RS12065 (LLKF_2404)	-	2460344..2461483 (-)	1140	WP_012898644.1	MFS transporter	-
LLKF_RS12070 (LLKF_2405)	kwcM	2461480..2462799 (-)	1320	WP_012898645.1	KxxxW cyclic peptide radical SAM maturase	-
LLKF_RS13310 (LLKF_2406)	-	2462867..2462980 (-)	114	WP_011836076.1	KxxxW-cyclized peptide pheromone	-
LLKF_RS12075 (LLKF_2407)	sepM	2463302..2464330 (-)	1029	WP_003132657.1	SepM family pheromone-processing serine protease	Regulator
LLKF_RS12080 (LLKF_2408)	coaD	2464317..2464814 (-)	498	WP_010906348.1	pantetheine-phosphate adenylyltransferase	-
LLKF_RS12085 (LLKF_2409)	rsmD	2464862..2465401 (-)	540	WP_003132655.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
LLKF_RS12090 (LLKF_2410)	-	2465572..2466792 (-)	1221	WP_012898647.1	MDR family MFS transporter	-
LLKF_RS12095 (LLKF_2411)	lmrP	2466876..2468102 (-)	1227	WP_012898648.1	multidrug efflux MFS transporter LmrP	-
LLKF_RS12100 (LLKF_2412)	-	2468290..2468784 (+)	495	WP_012898649.1	RNA polymerase sigma factor	-

Sequence

Protein

• Length: 342 a.a.
• Molecular weight: 37349.96 Da
• Isoelectric Point: 9.9183

>NTDB_id=35932 LLKF_RS12075 WP_003132657.1 2463302..2464330(-) (sepM) [Lactococcus lactis subsp. lactis KF147]
MKKNKKINPKLKWGISIGLIVVALLALFYPTSYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYSH
FNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAIYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELADTITAV
NGQQFTSSADMIAYVSKQKVGDSVTIEYTRIDGTKHKSTGKYIKIANGKTGIGISLVDHTEVVTTPKVTVNAGSIGGPSA
GMMFTLEIYSQLTGKDLRNGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAKK
LKTKMKIVPVKTIQDALDYLEK

Nucleotide

• Length: 1029 bp

>NTDB_id=35932 LLKF_RS12075 WP_003132657.1 2463302..2464330(-) (sepM) [Lactococcus lactis subsp. lactis KF147]
ATGAAAAAAAATAAAAAAATTAATCCCAAATTAAAATGGGGAATCTCGATTGGTCTAATTGTTGTTGCCCTTTTAGCTTT
ATTTTATCCAACGAGTTACTATGTGGAAATGCCCGGAACAACCGAGCCTTTAGGGAAAATGGTCAAAGTTGAAGGCAAAA
AAGATGAACATAAAGGTGATTTTTTTCTGACGACTGTCCAAATTGCACGAGCAAATCTTGCTACAATGATTTACAGCCAT
TTTAATAGTTTTACAAGCATTTACAGTGAGCAAGAAATGACTGGTGGACTTAACGACGCACAGTTCAACCGTGTCAACCA
GTTTTACATGGAAACGGCACAGAATACAGCGATTTATCAAGCTTTTAAATTAGCTAATAAACCTTATGAACTTAAATATG
AAGGCGTTTATGTCCTTGATATTGCCAAAAATTCGACCTTTAAAAATAAGCTAGAACTTGCAGATACGATTACAGCAGTC
AATGGTCAACAGTTTACATCAAGTGCCGACATGATTGCTTATGTTTCCAAACAAAAAGTCGGTGATTCAGTAACGATTGA
ATACACGAGAATTGACGGCACAAAGCACAAGTCAACTGGCAAATACATCAAAATTGCTAATGGAAAAACAGGAATTGGGA
TCAGCCTAGTTGACCATACTGAAGTCGTGACGACTCCAAAAGTTACTGTCAACGCGGGTTCAATCGGTGGTCCCTCTGCG
GGGATGATGTTTACACTAGAAATTTACAGTCAGTTGACAGGAAAAGATTTGCGAAACGGTCGCGAAATTGCTGGAACTGG
GACGATTGAACATGACGGAAGCATTGGCCAAATCGGCGGTGTTGATAAAAAAGTAGCCACAGCGAGCAAAGAAGGGGCAA
AAGTCTTCTTAGTGCCTGACTCAGGCACCAAAAAAGAAAGTAGCAACAATTACCTTGGAGCCAAAGCCGCCGCTAAAAAA
CTAAAAACGAAGATGAAAATCGTTCCCGTCAAAACTATTCAGGACGCTTTAGATTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

• PDZ domain (PDZ_2) (134-194)

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (227-338)

3D structure

• AlphaFold DB: S6FVM6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sepM	Streptococcus mutans UA159	48.696	100	0.491