Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LLKF_RS11560 Genome accession   NC_013656
Coordinates   2368268..2369632 (-) Length   454 a.a.
NCBI ID   WP_012898599.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis KF147     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2363268..2374632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLKF_RS11525 (LLKF_2302) - 2363814..2364308 (+) 495 WP_012898594.1 GNAT family N-acetyltransferase -
  LLKF_RS11530 (LLKF_2303) - 2364401..2364724 (-) 324 WP_003129893.1 DUF1048 domain-containing protein -
  LLKF_RS11535 (LLKF_2304) - 2364711..2365058 (-) 348 WP_012898595.1 DUF1048 domain-containing protein -
  LLKF_RS11540 (LLKF_2305) - 2365045..2365407 (-) 363 WP_012898596.1 PadR family transcriptional regulator -
  LLKF_RS11545 (LLKF_2306) - 2365528..2366157 (-) 630 WP_003129888.1 hypothetical protein -
  LLKF_RS11550 (LLKF_2307) - 2366222..2367307 (-) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  LLKF_RS11555 (LLKF_2308) - 2367538..2368224 (+) 687 WP_012898598.1 YoaK family protein -
  LLKF_RS11560 (LLKF_2309) radA 2368268..2369632 (-) 1365 WP_012898599.1 DNA repair protein RadA Machinery gene
  LLKF_RS11565 (LLKF_2310) - 2369794..2370021 (-) 228 WP_010906294.1 heavy-metal-associated domain-containing protein -
  LLKF_RS11570 (LLKF_2311) - 2370149..2371162 (-) 1014 WP_012898600.1 glycosyltransferase family 2 protein -
  LLKF_RS11575 (LLKF_2312) - 2371144..2371689 (-) 546 WP_012898601.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  LLKF_RS11580 (LLKF_2313) - 2371686..2372261 (-) 576 WP_012898602.1 SIS domain-containing protein -
  LLKF_RS11585 (LLKF_2314) - 2372325..2373275 (-) 951 WP_235187818.1 GHMP kinase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49980.36 Da        Isoelectric Point: 5.1748

>NTDB_id=35919 LLKF_RS11560 WP_012898599.1 2368268..2369632(-) (radA) [Lactococcus lactis subsp. lactis KF147]
MAKKKSSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEV
ERLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGE
RNNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNA
KRTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEI
RRVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=35919 LLKF_RS11560 WP_012898599.1 2368268..2369632(-) (radA) [Lactococcus lactis subsp. lactis KF147]
ATGGCGAAGAAAAAATCATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAA
CTGTGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAAC
GTTCAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACTTAGATGAATTTAATCGT
GTATTAGGAGGGGGCGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTT
ACAGGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAA
GAGCTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTT
GAGCGCTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTC
TGTAAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTC
ATGTGACTAAAGAAGGACAGCTGGCTGGGCCAAGGATGTTAGAGCATATGGTTGATACGGTGCTGTATTTTGAAGGAGAA
CGAAATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCA
GGGACATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCGA
TTGTTTGTGCACTTGAGGGAACGCGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCT
AAAAGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGAT
GCAAAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTG
CTGTGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATT
CGTCGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCAAAAAATTC
AATTGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTG
GCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(361-426)

AAA domain (AAA_25)

(71-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.057

100

0.811
  radA Streptococcus pneumoniae D39

81.057

100

0.811
  radA Streptococcus pneumoniae R6

81.057

100

0.811
  radA Streptococcus pneumoniae TIGR4

81.057

100

0.811
  radA Streptococcus mitis SK321

81.057

100

0.811
  radA Streptococcus mitis NCTC 12261

80.837

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.354

100

0.619

Multiple sequence alignment