Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LLKF_RS05670 Genome accession   NC_013656
Coordinates   1124185..1125507 (-) Length   440 a.a.
NCBI ID   WP_012897706.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis KF147     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1119185..1130507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLKF_RS05640 (LLKF_1111) - 1120328..1121296 (+) 969 WP_003130649.1 PhoH family protein -
  LLKF_RS05645 (LLKF_1112) - 1121354..1121833 (+) 480 WP_003130650.1 NUDIX hydrolase -
  LLKF_RS05650 (LLKF_1113) ybeY 1121985..1122473 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  LLKF_RS05655 (LLKF_1114) - 1122457..1122912 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  LLKF_RS05660 (LLKF_1115) - 1122926..1123504 (+) 579 WP_012897704.1 nucleotidyltransferase family protein -
  LLKF_RS13730 comFC 1123538..1123942 (-) 405 WP_226898158.1 ComF family protein Machinery gene
  LLKF_RS05670 (LLKF_1117) comFA 1124185..1125507 (-) 1323 WP_012897706.1 DEAD/DEAH box helicase Machinery gene
  LLKF_RS05675 (LLKF_1118) - 1125564..1126193 (+) 630 WP_012897707.1 YigZ family protein -
  LLKF_RS05680 (LLKF_1119) - 1126327..1126824 (+) 498 WP_012897708.1 VanZ family protein -
  LLKF_RS05685 (LLKF_1120) - 1126872..1127891 (+) 1020 WP_012897709.1 DUF475 domain-containing protein -
  LLKF_RS05690 (LLKF_1121) - 1128001..1128753 (+) 753 WP_012897710.1 bifunctional metallophosphatase/5'-nucleotidase -
  LLKF_RS05695 (LLKF_1122) glyQ 1129039..1129992 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50205.70 Da        Isoelectric Point: 10.1065

>NTDB_id=35900 LLKF_RS05670 WP_012897706.1 1124185..1125507(-) (comFA) [Lactococcus lactis subsp. lactis KF147]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVLYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSSERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=35900 LLKF_RS05670 WP_012897706.1 1124185..1125507(-) (comFA) [Lactococcus lactis subsp. lactis KF147]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTACTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATCATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTTCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-404)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38

Multiple sequence alignment