Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   FBF69_RS04550 Genome accession   NZ_CP039453
Coordinates   932593..934026 (-) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis strain 220     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 927593..939026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBF69_RS04520 (FAI36_04525) elbB 929126..929779 (+) 654 WP_136484907.1 isoprenoid biosynthesis glyoxalase ElbB -
  FBF69_RS04525 (FAI36_04530) mtgA 929776..930501 (+) 726 WP_136484909.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  FBF69_RS04530 (FAI36_04535) npr 930540..930812 (-) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  FBF69_RS04535 (FAI36_04540) rapZ 930809..931663 (-) 855 WP_014885397.1 RNase adapter RapZ -
  FBF69_RS04540 (FAI36_04545) ptsN 931709..932200 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  FBF69_RS04545 (FAI36_04550) hpf 932283..932570 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  FBF69_RS04550 (FAI36_04555) rpoN/rpoN1 932593..934026 (-) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  FBF69_RS04555 (FAI36_04560) lptB 934074..934799 (-) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  FBF69_RS04560 (FAI36_04565) lptA 934806..935360 (-) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  FBF69_RS04565 (FAI36_04570) lptC 935329..935904 (-) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  FBF69_RS04570 (FAI36_04575) kdsC 935901..936467 (-) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  FBF69_RS04575 (FAI36_04580) kdsD 936482..937468 (-) 987 WP_136484911.1 arabinose-5-phosphate isomerase KdsD -
  FBF69_RS04580 (FAI36_04585) - 937482..938459 (-) 978 WP_028014566.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=358733 FBF69_RS04550 WP_028014571.1 932593..934026(-) (rpoN/rpoN1) [Enterobacter bugandensis strain 220]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=358733 FBF69_RS04550 WP_028014571.1 932593..934026(-) (rpoN/rpoN1) [Enterobacter bugandensis strain 220]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCCATTGCCACCTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCGAAGGACCTGCGCGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCTCGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTGACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATCCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTGGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCCTTCTTTGAGCAGGGCGAAGAGTATATGAAGCCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment