Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   E8M03_RS08490 Genome accession   NZ_CP039448
Coordinates   1709164..1709850 (-) Length   228 a.a.
NCBI ID   WP_078055782.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VGC1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1704164..1714850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M03_RS08470 (E8M03_08560) rpsT 1705078..1705329 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  E8M03_RS08475 (E8M03_08565) holA 1705374..1706348 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  E8M03_RS08480 (E8M03_08570) comEC 1706405..1708606 (-) 2202 WP_000967504.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  E8M03_RS08485 (E8M03_08575) - 1708611..1709072 (-) 462 WP_000439693.1 ComE operon protein 2 -
  E8M03_RS08490 (E8M03_08580) comEA 1709164..1709850 (-) 687 WP_078055782.1 ComEA family DNA-binding protein Machinery gene
  E8M03_RS08495 (E8M03_08585) - 1709890..1710606 (-) 717 WP_000942456.1 class I SAM-dependent methyltransferase -
  E8M03_RS08500 (E8M03_08590) rsfS 1710609..1710962 (-) 354 WP_001088020.1 ribosome silencing factor -
  E8M03_RS08505 (E8M03_08595) yqeK 1710963..1711547 (-) 585 WP_001017838.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  E8M03_RS08510 (E8M03_08600) nadD 1711537..1712106 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  E8M03_RS08515 (E8M03_08605) yhbY 1712109..1712399 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  E8M03_RS08520 (E8M03_08610) aroE 1712403..1713209 (-) 807 WP_000666767.1 shikimate dehydrogenase -
  E8M03_RS08525 (E8M03_08615) yqeH 1713223..1714323 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25761.45 Da        Isoelectric Point: 9.6753

>NTDB_id=358676 E8M03_RS08490 WP_078055782.1 1709164..1709850(-) (comEA) [Staphylococcus aureus strain VGC1]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=358676 E8M03_RS08490 WP_078055782.1 1709164..1709850(-) (comEA) [Staphylococcus aureus strain VGC1]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCTAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTGCTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
GAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.678

99.561

0.982
  comEA Staphylococcus aureus N315

98.678

99.561

0.982

Multiple sequence alignment