Detailed information NTG0358523

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: F3052_RS00860
• Genome accession: NZ_CP047277
• Coordinates: 181420..182403 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain N18-04097
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 176420..187403

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F3052_RS00845 (F3052_00850)	dppA	177563..179170 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
F3052_RS24710	-	179204..179314 (-)	111	Protein_170	hypothetical protein	-
F3052_RS00850 (F3052_00855)	dppB	179478..180497 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
F3052_RS00855 (F3052_00860)	dppC	180507..181409 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
F3052_RS00860 (F3052_00865)	amiE	181420..182403 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
F3052_RS00865 (F3052_00870)	dppF	182400..183404 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
F3052_RS00870 (F3052_00875)	yhjV	183434..184705 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
F3052_RS00875 (F3052_00880)	ldrD	185181..185288 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-
F3052_RS00880 (F3052_00885)	bcsG	185375..187054 (-)	1680	WP_000191622.1	cellulose biosynthesis protein BcsG	-
F3052_RS00885 (F3052_00890)	bcsF	187051..187242 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=358523 F3052_RS00860 WP_001196486.1 181420..182403(+) (amiE) [Escherichia coli strain N18-04097]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=358523 F3052_RS00860 WP_001196486.1 181420..182403(+) (amiE) [Escherichia coli strain N18-04097]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404