Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E7T04_RS00190 Genome accession   NZ_CP039434
Coordinates   42755..44125 (+) Length   456 a.a.
NCBI ID   WP_002356050.1    Uniprot ID   -
Organism   Enterococcus faecalis strain SGAir0397     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 37755..49125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7T04_RS00165 (E7T04_00165) - 38245..38712 (-) 468 WP_002408153.1 VOC family protein -
  E7T04_RS00170 (E7T04_00170) - 38777..39724 (+) 948 WP_002389777.1 YafY family protein -
  E7T04_RS00175 (E7T04_00175) - 39816..41069 (-) 1254 WP_002385467.1 glutamate-5-semialdehyde dehydrogenase -
  E7T04_RS00180 (E7T04_00180) proB 41056..41871 (-) 816 WP_002389550.1 glutamate 5-kinase -
  E7T04_RS00185 (E7T04_00185) - 42191..42676 (+) 486 WP_002367569.1 dUTP diphosphatase -
  E7T04_RS00190 (E7T04_00190) radA 42755..44125 (+) 1371 WP_002356050.1 DNA repair protein RadA Machinery gene
  E7T04_RS00195 (E7T04_00195) - 44226..45371 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  E7T04_RS00200 (E7T04_00200) ispF 45390..45863 (+) 474 WP_002367570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  E7T04_RS00205 (E7T04_00205) gltX 45916..47373 (+) 1458 WP_071646502.1 glutamate--tRNA ligase -
  E7T04_RS00215 (E7T04_00215) epsC 47643..48182 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49757.28 Da        Isoelectric Point: 7.3489

>NTDB_id=358478 E7T04_RS00190 WP_002356050.1 42755..44125(+) (radA) [Enterococcus faecalis strain SGAir0397]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=358478 E7T04_RS00190 WP_002356050.1 42755..44125(+) (radA) [Enterococcus faecalis strain SGAir0397]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGACGCTGTCCTAA
TTGCGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTTGTACCTAAAAAAGAGCCGCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCATTAGTTTTAATCGGTGGCGATCCCGGAATTGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTGCGAGCGGAGCGTCTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGATTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAGGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGAATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACAAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACTGGGT
CTTCGATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCGGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATTCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGTATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.385

99.781

0.752
  radA Streptococcus pneumoniae Rx1

75.385

99.781

0.752
  radA Streptococcus pneumoniae D39

75.385

99.781

0.752
  radA Streptococcus pneumoniae R6

75.385

99.781

0.752
  radA Streptococcus pneumoniae TIGR4

75.385

99.781

0.752
  radA Streptococcus mitis SK321

75.385

99.781

0.752
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656

Multiple sequence alignment